Diversity analysis of culturable bacteria isolated from marine sediments of Arctic

Wang Zhen; Li Yang; Che Shuai; Lin Xuezheng

doi:10.3969/j.issn.0253-4193.2014.10.012

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Wang Zhen, Li Yang, Che Shuai, Lin Xuezheng. Diversity analysis of culturable bacteria isolated from marine sediments of Arctic[J]. Haiyang Xuebao, 2014, 36(10): 116-123. doi: 10.3969/j.issn.0253-4193.2014.10.012

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Wang Zhen, Li Yang, Che Shuai, Lin Xuezheng. Diversity analysis of culturable bacteria isolated from marine sediments of Arctic[J]. Haiyang Xuebao, 2014, 36(10): 116-123. doi: 10.3969/j.issn.0253-4193.2014.10.012

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Diversity analysis of culturable bacteria isolated from marine sediments of Arctic

doi: 10.3969/j.issn.0253-4193.2014.10.012

Key Lab of Marine Bioactive Substances State Oceanic Administration, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2014-03-16
Rev Recd Date: 2014-04-08

Abstract

Abstract

Culturable bacteria were isolated by use of marine Zobell 2216E medium and spread plate method. Molecular identification and phylogenetic analysis were carried out on the basis of 16S rRNA gene. According to the distinct morphological character of bacterial colony on the marine Zobell 2216E medium plate,a total of 570 isolates were obtained from Arctic sediments. Molecular identification and phylogenetic analysis showed that these isolates belonged to four phylum of the bacteria domain,5 classes,47 genera,and 102 species in total. 14 isolates had 16S rRNA gene similarity less than 97% with the closest type strain were grouped into 6 clusters. It indicated that these isolates might be potential novel species. A high diversity of culturable bacteria were isolated from marine sediment of Arctic in term of both species number and phylogenetic composition,which deserves further study and exploitation,such as novel bioactive substances and functional genes.
- Arctic,
- marine sediment,
- bacterial diversity,
- phylogenetic analysis

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References(20)

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