Chromosome composition and AFLP analysis of <em>Pagrosomus major</em>, <em>Spraus macrocephalus</em> and the hybirds

Lin Mian; Miao Liang; Li Mingyun; Yu Yinyin; Xu Wantu

doi:10.3969/j.issn.0253-4193.2014.08.013

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Lin Mian, Miao Liang, Li Mingyun, Yu Yinyin, Xu Wantu. Chromosome composition and AFLP analysis of Pagrosomus major, Spraus macrocephalus and the hybirds[J]. Haiyang Xuebao, 2014, 36(8): 117-121. doi: 10.3969/j.issn.0253-4193.2014.08.013

Citation:

Lin Mian, Miao Liang, Li Mingyun, Yu Yinyin, Xu Wantu. Chromosome composition and AFLP analysis of Pagrosomus major, Spraus macrocephalus and the hybirds[J]. Haiyang Xuebao, 2014, 36(8): 117-121. doi: 10.3969/j.issn.0253-4193.2014.08.013

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Chromosome composition and AFLP analysis of Pagrosomus major, Spraus macrocephalus and the hybirds

doi: 10.3969/j.issn.0253-4193.2014.08.013

1.
Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China
2.
Gangwan Aquacultural Seeding Co. Ltd. of Xiangshan County, Xiangshan 315702, China

Received Date: 2013-07-25
Rev Recd Date: 2013-10-19

Abstract

Abstract

In this paper,the karyotyping analysis and AFLP molecular marker technique were used to analyze the genetic differences among Pagrosomus major,Sparus macrocephalus and the hybrids.The karyotype analysis showed that they all contained 48 chromosomes and the karyotypes were respectively: 2n=2st+46t,NF=48; 2n=4m+4sm+38t+2st,NF=56; 2n=2st+8t+8sm+30m=48,NF=86.The karyotype of the hybrids was different from maternal and paternal species.AFLP analysis of P.major,S.macrocephalus and the hybrids showed that a total of 278 bands were obtained by 2 primer combinations,including 93 paternal species (S.macrocephalus) specific bands and 108 maternal species (P.major) specific bands.Among these parent specific bands,21 male parent specific bands and 67 female parent specific bands were detected in the hybrids.In addition,there were 15 nonparental bands appeared in the hybrids.Analysis of molecular variance revealed that the Nei's unbiased measures of genetic identity were 0.113 between the hybrids and S.macrocephalus and 0.350 between the hybrids and P.major,respectively.The Nei's unbiased measures of genetic distance were 2.180 between the hybrids and S.macrocephalus and 1.050 between the hybrids and P.major,respectively.This study indicated that the hybrids of P.major♀ and S.macrocephalus♂ were heterologous diploid containing 48 chromosomes and the genetic material of maternal and paternal species was partly reorganized during the hybridization and showed a partially maternal hereditary characteristics.
- crossbreeding,
- Pagrosomus major,
- Spraus macrocephalus,
- karyotype,
- AFLP

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References(28)

References

徐恭绍,郑澄伟.海产鱼类养殖与增殖[M].济南: 山东科学技术出版社,1987: 322-345.

江世贵,李加儿,区又君,等.平鲷♀与真鲷♂杂交研究[J].海洋科学,1997(5): 33-38.

单保当,洪万树,何大仁.真鲷与黑鲷杂交与多倍体育种系列研究-Ⅰ[J].海洋渔业,1999,21(1): 14-15.

蒋宏雷,吴雄飞,沈庞幼.真鲷与黑鲷杂交繁育技术研究[J].渔业现代化,2008,35(6): 33-35,60.

姜景腾,吴雄飞,蒋宏雷.低氧胁迫对真鲷(♀)与黑鲷(♂)杂交子一代体内酶活力的影响[J].宁波大学学报(理工版),2010,23(4): 10-14.

吴仲庆.水产生物遗传育种学[M].3版.厦门: 厦门大学出版社,2000: 7-25.

周贺,秦艳杰,周伯文,等.4种扇贝AFLP分析体系的建立[J].大连水产学院学报,2009,24(S1): 56-58.

安丽,刘萍,李健,等."黄海1号"中国对虾不同世代间的AFLP分析[J].中国海洋大学学报(自然科学版),2008,38(6): 921-926.

Liu Z,Nichols A,Li P,et al.Inheritance and usefulness of AFLP markers in channel catfish (Ictalurus punctatus),blue catfish (I.furcatus),and their F1,F2,and backcross hybrids[J].Molecular and General Genetics,1998,258(3): 260-268.

Agresti J J,Seki S,Cnaani A,et al.Breeding new strains of tilapia: development of an artificial center of origin and linkage map based on AFLP and microsatellite loci[J].Aquaculture,2000,185(1/2): 43-56.

王伟继,孔杰,董世瑞,等.中国明对虾AFLP分子标记遗传连锁图谱的构建[J].动物学报,2006,52(3): 575-584.

任军,黄路生,艾华水,等.24个中外猪种(群)的AFLP多态性及其群体遗传关系[J].遗传学报,2002,29(9): 774-781.

Lallias D,Lapègue S,Hecquet C,et al.AFLP-based genetic linkage maps of the blue mussel (Mytilus edulis)[J].Animal Genetics,2007,38(4): 340-349.

Guo J,Jiang R H Y,Kamphuis L G,et al.A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans[J].Fungal Genetics and Biology,2006,43(2): 111-123.

Levan A,Fredga K,Sandberg A A.Nomenclature for centromeric position on chromosomes[J].Hereditas,1964,52(2): 201-220.

Corman G C.The chromosomes of the Reptilia,a cytotaxonomic interpretation [C]//Chiarelli A B,Capanna E.Cytotaxonomy and Vertebrate Evolution.New York: Academic Press Inc,1973: 5-30.

王志勇,王艺磊,林利民,等.利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异[J].水产学报,2001,25(4): 289-293.

严骏骢,赵金良,李思发,等.鳙中国土著群体与移居群体遗传变异的AFLP分析[J].中国水产科学,2011,18(2): 283-289.

王晓清,王志勇,谢中国,等.大黄鱼(♀)与鮸(♂)杂交的遗传分析[J].水产学报,2008,32(1): 51-57.

黄帆.高加索三叶草与白三叶杂交后代遗传特性及育性恢复的研究 [D].呼和浩特: 内蒙古农业大学,2012.

周丽清,杨爱国,刘志鸿,等.栉孔扇贝×虾夷扇贝杂交子一代与双亲染色体核型的分析[J].水生生物学报,2005,29(1): 105-109.

杨爱国,王清印,刘志鸿,等.栉孔扇贝与虾夷扇贝杂交及子一代的遗传性状[J].海洋水产研究,2004,25(5): 1-5.

隋班良,蔡明夷,刘颖,等.黄姑鱼♀与大黄鱼♂杂交试验与AFLP分析[J].集美大学学报(自然科学版),2012,17(4): 241-246.

万俊芬,包振民,刘广绪,等.扇贝种间单对杂交一代幼虫ISSR标记的分离方式[J].高技术通讯,2004,14(5): 82-87.

何斌,杨爱国,王清印,等.栉孔扇贝×虾夷扇贝单对杂交子一代幼虫ISSR标记的分离方式[J].海洋水产研究,2007,28(6): 32-38.

Ayliffe M A,Lawrence G J,Ellis J G,et al.Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands[J].Nucleic Acids Research,1994,22(9): 1632-1636.

房经贵,章镇,马正强,等.AFLP标记在两个芒果品种间杂交F1代的多态性及分离方式[J].中国农业科学,2000,33(3): 19-24.

姜景腾.真鲷♀×黑鲷♂杂交子一代抗逆性初步研究 [D].宁波: 宁波大学,2011.

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