Phylogenetic analysis based on <i>hsp</i>70 gene sequences of the nine red seaweeds from Chinese coasts

YANG Rui; WANG Shugang; CHEN Juanjuan; WANG Yajun; XU Lining; SUN Xue

doi:10.3969/j.issn.0253-4193.2013.04.022

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YANG Rui, WANG Shugang, CHEN Juanjuan, WANG Yajun, XU Lining, SUN Xue. Phylogenetic analysis based on hsp70 gene sequences of the nine red seaweeds from Chinese coasts[J]. Haiyang Xuebao, 2013, 35(4): 188-200. doi: 10.3969/j.issn.0253-4193.2013.04.022

Citation:

YANG Rui, WANG Shugang, CHEN Juanjuan, WANG Yajun, XU Lining, SUN Xue. Phylogenetic analysis based on hsp70 gene sequences of the nine red seaweeds from Chinese coasts[J]. Haiyang Xuebao, 2013, 35(4): 188-200. doi: 10.3969/j.issn.0253-4193.2013.04.022

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Phylogenetic analysis based on hsp70 gene sequences of the nine red seaweeds from Chinese coasts

doi: 10.3969/j.issn.0253-4193.2013.04.022

1.
Ningbo University, Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Zhejiang, Ningbo 315211, China;School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, China
2.
College of Science & Technology Ningbo University, Zhejiang, Ningbo 315211, China

Received Date: 2012-07-02
Rev Recd Date: 2012-12-20

Abstract

Abstract

Nine common species of red seaweeds were collected from the coasts of Qingdao Shandong, Nantong Jiangsu and Xiangshan Zhejiang area, which could be classified into 2 classes, 5 orders, 6 families, 8 genus (according to the NCBI). The hsp 70 genes were amplified by PCR from 9 red seaweeds, phylogenetic analysis were carried out based on the hsp70 gene and HSP 70 protein sequences. The obtained target bands amplified by the primers P1/P3 were about 630bp, which were presumed located near the ATPs domain based on the HSPs sequences analysis The distances of hsp70 sequences within 9 red seaweeds were between 0.078 and 0.319, and the similarities of sequences were between 73% and 92%, which conservation were slightly lower than their HSP70 proteins. The codons ended with A and T were preferred in the hsp70 genes of these 9 red seaweeds while the codons of CGC and TGG were not founded. The results of the phylogenetic analysis of hsp70, 18S rDNA and rbcL sequences in other algae, microbes and plants obtained from GenBank suggested that the origin of the primitive red algae Cyanidium caldarium and Cyanidioschyzon merolae strain 10D were more primordially than the other red algae, while the Pyropia species were followed, and the phylogenesis of the 9 species of red seaweeds studied in this paper were coherent with those described in NCBI. The difference of nutrition mode might be the basic factor of HSP 70 differentiation in fungi, algae and plant, while the adaptation to variable environment was the major factor in biological molecular evolution when the organisms were at the same morphological levels, which made the species much stronger in stress resistance and more stable in genetic structures.
- red seaweeds,
- hsp70,
- phylogeny

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