Screening, cloning and bioinformatics analysis of P23 gene in <i>Litopenaeus vannamei</i>

LI Xilian; ZHANG Yanyan; XIN Jingjing; QIAN Zhaoyin; LIU Xiaolin; XIANG Jianhai

doi:10.3969/j.issn.0253-4193.2013.04.019

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LI Xilian, ZHANG Yanyan, XIN Jingjing, QIAN Zhaoyin, LIU Xiaolin, XIANG Jianhai. Screening, cloning and bioinformatics analysis of P23 gene in Litopenaeus vannamei[J]. Haiyang Xuebao, 2013, 35(4): 162-167. doi: 10.3969/j.issn.0253-4193.2013.04.019

Citation:

LI Xilian, ZHANG Yanyan, XIN Jingjing, QIAN Zhaoyin, LIU Xiaolin, XIANG Jianhai. Screening, cloning and bioinformatics analysis of P23 gene in Litopenaeus vannamei[J]. Haiyang Xuebao, 2013, 35(4): 162-167. doi: 10.3969/j.issn.0253-4193.2013.04.019

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Screening, cloning and bioinformatics analysis of P23 gene in Litopenaeus vannamei

doi: 10.3969/j.issn.0253-4193.2013.04.019

1.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China;Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang 313001, China
2.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China
3.
Experimental Marine Biology Laboratory, Institute of Ocean logy, Chinese Academy of Sciences, Qingdao 266071, China

Received Date: 2012-06-19
Rev Recd Date: 2013-04-11

Abstract

Abstract

Screening, cloning and bioinformatics analysis of P23 gene differently expressed in large female and small female of Litopenaeus vannamei.Suppression subtractive hybridization (SSH) approach was used to isolate differently expressed genes in abdominal muscle samples of the female-shrimp between large female (LF; body weight>90 percentile of weight distribution curve) and small female (SF; body weight<10 percentile of weight distribution curve). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was used to analysis the change of P23 mRNA level between large female and small female, and biotechnology were employed to predict gene specific structure and potential motif domain.The subtraction efficiency was estimated by a housekeeping gene β-actin, and the results showed that β-actin was subtracted efficiently at 2¹⁰ and 2⁵ folds for SF and LF subtracted cDNA library respectively. RT-QPCR showed that the P23 has an effect to up-regulated expression in weight gain. Whole length of the P23 gene from Litopenaeus vannamei was 495bp (186 amino acid residues). The sequence has been accessed in GenBank (JF806619). Partial sequence of the P23 gene had strong hydrophobicity but with no transmembrane helices structure. The signal peptide prediction in P23 gene showed that there was a signal peptide with strong amino acid residues in the partial Sequence of the P23 gene．
- Litopenaeus vannamei,
- P23 gene,
- bioinformatic,
- SSH

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References(17)

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