Exploring the molecular regulatory mechanisms of planktonic larvae development in <i>Mytilus coruscus</i> using weighted gtene co-expression network analysis and time-series differential analysis

Li Zhong; Wei Xuelian; Liu Wanting; Li Jiji; Ye Yingying

doi:10.12284/hyxb2025072

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Li Zhong，Wei Xuelian，Liu Wanting, et al. Exploring the Molecular regulatory mechanisms of planktonic larvae development in Mytilus coruscus using weighted gene co-expression network analysis and time-series differential analysis[J]. Haiyang Xuebao，2025, 47(7)：1–15 doi: 10.12284/hyxb2025072

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Li Zhong，Wei Xuelian，Liu Wanting, et al. Exploring the Molecular regulatory mechanisms of planktonic larvae development in Mytilus coruscus using weighted gene co-expression network analysis and time-series differential analysis[J]. Haiyang Xuebao，2025, 47(7)：1–15 doi: 10.12284/hyxb2025072

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Exploring the molecular regulatory mechanisms of planktonic larvae development in Mytilus coruscus using weighted gtene co-expression network analysis and time-series differential analysis

doi: 10.12284/hyxb2025072

Li Zhong^1
,,
Wei Xuelian^1
,,
Liu Wanting¹,
Li Jiji¹,
Ye Yingying^{1
,
,}

National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University , Zhoushan 316022, China

Received Date: 2024-10-18
Rev Recd Date: 2025-03-13

Available Online: 2025-04-28

Abstract

Abstract

Mytilus coruscus is renowned for its delicious taste and rich nutritional content, making it one of the significant economic shellfish along the coast of China. The critical period for M. coruscus seedling cultivation lies in its larval metamorphosis stage. However, the larval development of M. coruscus is a dynamic and complex process, involving the participation of numerous genes and various intricate biological processes. In this study, we based on high-throughput transcriptome sequencing technology, conducted transcriptome sequencing on larval samples of M. coruscus at five key developmental stages (Trocophore, D-veliger, Umbo, Pediveliger, and Post-larvae), identifying a total of 20 894 differentially expressed genes. Weighted gene co-expression network analysis and time-course difference analysis were performed on the differentially expressed genes, followed by joint analysis. Six key submodules conforming to specific temporal developmental patterns were selected, identifying a total of 2 395 genes. Gene Ontology enrichment analysis and protein network interaction analysis were conducted on genes within each submodule. Thirty hub genes related to the growth and development process of M. coruscus were identified, such as Fen1, Ndufab1b, Ndufs8a, Pcan, Rnaseh2a; Cdh1, Cacng4b, Cav1, Blm, Ryr1a; Mars1, Cdc42, Aasdh, Apoba, Cav1; Kif11, Cdc20, Ubc, Kif23, Cdc6; Ubc, Rps16, Rpl23, Rpsa, Rps27a; Cdc20, Setd2, Ssrp1a, Cav1, Rab8a. They playing major regulatory roles in larval development, including regulation of DNA replication and cell division processes, mitochondrial and ribosomal functions, and protein synthesis processes. This study explores the molecular mechanisms underlying the regulation of M. coruscus larval development at the transcriptome level, providing important theoretical guidance for studying functional genes in M. coruscus and subsequent molecular breeding practices aimed at cultivating new varieties with superior phenotypic traits.
- Mytilus coruscus,
- planktonic larvae,
- transcriptome,
- weighted gene correlation network analysis,
- time-course difference analysis

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References(65)

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