Reproductive regulation of <i>fruitless</i> gene in brine shrimp <i>Artemia franciscana</i>

Li Ke; Ren Yizhuo; Han Xuekai; Liu Xue; Sui Liying

doi:10.12284/hyxb2023139

Volume 45 Issue 10

Oct. 2023

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Li Ke，Ren Yizhuo，Han Xuekai, et al. Reproductive regulation of fruitless gene in brine shrimp Artemia franciscana[J]. Haiyang Xuebao，2023, 45(10)：114–122 doi: 10.12284/hyxb2023139

Citation:

Li Ke，Ren Yizhuo，Han Xuekai, et al. Reproductive regulation of fruitless gene in brine shrimp Artemia franciscana[J]. Haiyang Xuebao，2023, 45(10)：114–122 doi: 10.12284/hyxb2023139

Li Ke，Ren Yizhuo，Han Xuekai, et al. Reproductive regulation of fruitless gene in brine shrimp Artemia franciscana[J]. Haiyang Xuebao，2023, 45(10)：114–122 doi: 10.12284/hyxb2023139

Citation:

Li Ke，Ren Yizhuo，Han Xuekai, et al. Reproductive regulation of fruitless gene in brine shrimp Artemia franciscana[J]. Haiyang Xuebao，2023, 45(10)：114–122 doi: 10.12284/hyxb2023139

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Reproductive regulation of fruitless gene in brine shrimp Artemia franciscana

doi: 10.12284/hyxb2023139

Li Ke^{1, 3
,},
Ren Yizhuo²,
Han Xuekai^{1, 3
,
,},
Liu Xue^{1, 3},
Sui Liying^{1, 3
,
,}

1.
Asia Regional Artemia Reference Center, Tianjin University of Science and Technology, Tianjin 300457, China
2.
Hebei Engineering Research Center for Ecological Restoration of Rivers and Coastal Areas, Hebei University of Environmental Engineering, Qinhuangdao 066102, China
3.
Key Laboratory of Marine Resource Chemistry and Food Technology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China

Received Date: 2023-04-09
Rev Recd Date: 2023-06-15

Available Online: 2023-12-27

Publish Date: 2023-10-30

Abstract

Abstract

fruitless (fru) gene plays an important role in courtship, mating behavior and reproductive development of insectsand crustaceans. Artemia is not only the crucial live food in fish and crustacean larviculture, but also an ideal experimental organism for biological study. In this experiment, the open reading frame (ORF) of fru gene was obtained from the transcriptome of Artemia franciscana and analyzed bioinformatically. qPCR was used to study the expression characteristics of this gene at different stages of gonad development in the Artemia, and its function was explored by RNAi microinjection. Bioinformatics analysis showed that the ORF length of fru gene was 1 215 bp, which contains 404 amino acids, while its molecular weight and isoelectric point were 45.19 kDa and 5.28, and it was an acidic hydrophilic protein with no signal peptide or transmembrane structure; structural domain prediction showed that there are two structural domains of fru, BTB_POZ and HTH. The secondary structure is dominated by α-helix and irregular coil, and the tertiary structure corresponds to it. The qPCR results showed that the expression of fru gene showed a significant increase in late embryos stage, and its expression was significantly higher than that of early oocyte, later oocyte and early embryo stages in the ovary (P < 0.01); while the expression of fru gene also increased in immature stage of the testis, and its expression was significantly higher than that of early, middle and late maturation stages (P < 0.01). After RNA interference, we found that there was a significant decrease in the expression of the fru gene (P < 0.01), and all the offspring produced were cysts. This suggests that the fru gene has an important effect on reproduction and development of A. franciscana, and may be a key factor influencing the reproductive mode of Artemia. Through this study, we obtained important information about the role of fru gene in the reproductive development of Artemia and their related molecular mechanisms, which can help us better understand this important biological process.
- Artemia,
- fru gene,
- gonad,
- gene expression character,
- RNAi,
- reproductive development

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References(36)

References

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