Identification of <i>17β-HSDs</i> gene family and their expression patterns in gonadal development of <i>Sinonovacula constricta</i>

Li Lin; Dong Yinghui; Fu Lulu; Meng Yiping; Lin Zhihua; Yao Hanhan

doi:10.12284/hyxb2023137

Volume 45 Issue 10

Oct. 2023

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Li Lin，Dong Yinghui，Fu Lulu, et al. Identification of 17β-HSDs gene family and their expression patterns in gonadal development of Sinonovacula constricta[J]. Haiyang Xuebao，2023, 45(10)：123–134 doi: 10.12284/hyxb2023137

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Li Lin，Dong Yinghui，Fu Lulu, et al. Identification of 17β-HSDs gene family and their expression patterns in gonadal development of Sinonovacula constricta[J]. Haiyang Xuebao，2023, 45(10)：123–134 doi: 10.12284/hyxb2023137

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Identification of 17β-HSDs gene family and their expression patterns in gonadal development of Sinonovacula constricta

doi: 10.12284/hyxb2023137

1.
Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
2.
Ninghai Marine Biological Seed Industry Research Institute, Ninghai 315604, China

Received Date: 2023-02-13
Rev Recd Date: 2023-04-26

Available Online: 2023-11-02

Publish Date: 2023-10-30

Abstract

Abstract

17β-hydroxysteroid dehydrogenases (17β-HSDs) are the oxidoreductases in the last step of sex steroid hormone synthesis process, and play important roles in animal gonadal development and reproductive regulation. In this study, on the basis of the genome data of the razor clam Sinonovacula constricta, 17β-HSD genes were cloned and identified, and their chromosome distributions, phylogenetic relationships and gene expression patterns in different tissues and under different gonadal developmental stages were analyzed. The results showed that 17β-HSD6, 17β-HSD10, 17β-HSD12 and 17β-HSD14 genes of S. constricta were located on four different chromosomes, and the encoded proteins all contained the conserved SDR superfamily domains. The four 17β-HSD proteins were clearly differentiated and clustered into four independent branches in the phylogenetic trees. The results of qRT-PCR showed that 17β-HSD genes were all expressed in testes tissues, and possessed higher expression levels in gonads and hepatopancreas. During the gonadal development of S. constricta, 17β-HSD6 gene was expressed higher in testis and ovary at the spawning stage. The expression levels of 17β-HSD10, 17β-HSD12 and 17β-HSD14 genes showed a trend of increasing first and then decreasing during the development cycle of testis and ovary, which were all expressed higher at the mature stage. At the mature stage, 17β-HSD10 gene was expressed significantly higher in testis than in ovary (p < 0.05) , while the expression levels of 17β-HSD12 and 17β-HSD14 genes were all significantly higher in ovary than in testis (p < 0.05). Therefore, 17β-HSD6 gene might not be involved in regulating gonadal development process, while the expressions of 17β-HSD10, 17β-HSD12 and 17β-HSD14 genes were closely related to the gonadal development, suggesting that they might affect the gonadal development by regulating the synthesis and metabolism of sex steroid hormones in S. constricta. These findings laid theoretical foundations for further study on mechanism of 17β-HSD genes in regulating gonadal development and reproductive endocrine of mollusks.
- Sinonovacula constricta,
- 17β-HSDs gene family,
- gonadal development,
- expression analysis

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References(43)

References

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