Cloning of the <i>McNF-κB</i> gene of <i>Mytilus coruscus</i> and its role in development

Ren Hongyu; Liu Tiantian; Zhu Youting; Yang Jinlong; Liang Xiao

doi:10.12284/hyxb2023114

Volume 45 Issue 9

Sep. 2023

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Ren Hongyu，Liu Tiantian，Zhu Youting, et al. Cloning of the McNF-κB gene of Mytilus coruscus and its role in development[J]. Haiyang Xuebao，2023, 45(9)：141–151 doi: 10.12284/hyxb2023114

Citation:

Ren Hongyu，Liu Tiantian，Zhu Youting, et al. Cloning of the McNF-κB gene of Mytilus coruscus and its role in development[J]. Haiyang Xuebao，2023, 45(9)：141–151 doi: 10.12284/hyxb2023114

Ren Hongyu，Liu Tiantian，Zhu Youting, et al. Cloning of the McNF-κB gene of Mytilus coruscus and its role in development[J]. Haiyang Xuebao，2023, 45(9)：141–151 doi: 10.12284/hyxb2023114

Citation:

Ren Hongyu，Liu Tiantian，Zhu Youting, et al. Cloning of the McNF-κB gene of Mytilus coruscus and its role in development[J]. Haiyang Xuebao，2023, 45(9)：141–151 doi: 10.12284/hyxb2023114

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Cloning of the McNF-κB gene of Mytilus coruscus and its role in development

doi: 10.12284/hyxb2023114

Ren Hongyu^{1, 2
,},
Liu Tiantian^{1, 2},
Zhu Youting^{1, 2},
Yang Jinlong^{1, 2},
Liang Xiao^{1, 2
,
,}

1.
Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai Ocean University, Shanghai 201306, China
2.
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai 201306, China

Received Date: 2023-01-29
Rev Recd Date: 2023-04-20

Available Online: 2023-08-24

Publish Date: 2023-09-30

Abstract

Abstract

Nuclear factor κB (NF-κB) can regulate immunity, inflammation, apoptosis, cell proliferation, and organism development. At present, NF-κB has been well studied in vertebrates and fruit flies, while its role in shellfish is still elusive. In order to further explore the role of NF-κB in the immunity and development of mussel Mytilus coruscus, the full length McNF-κB cDNA sequence was cloned from M. coruscus. McNF-κB gene was 4087 bp long, and the open reading frame was 2 613 bp, encoding 871 amino acids and had a typical ankyrinrepeat (ANK) domain and DEATH domain. The results of amino acid sequence analysis showed that the gene had 72.76% homology with M. edulis and 66.58% with M. galloprovincialis, respectively, and was clustered with M. edulis and M. galloprovincialis in the phylogenetic tree. The real-time PCR (qRT-PCR) technology showed that the McNF-κB gene was expressed distributed in all tissues of M. coruscus, and the expression was the highest in the gill. McNF-κB gene was expressed in both the pediveliger larvae stage and the juvenile stage of M. coruscus, and the expression was significantly higher in the pediveliger larvae stage than that in the juvenile stage. After using RNA interference technology to silence the McNF-κB gene of pediveliger larvae, the larval metamorphosis rate decreased significantly, indicating that this gene was involved in regulating the metamorphosis process of M. coruscus. This study provides a basis for exploring how McNF-κB gene regulates development of M. coruscus.
- Mytilus coruscus,
- NF-κB,
- gene cloning,
- larval metamorphosis

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References(62)

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