Effect of the content of colanic acid in marine bacterial biofilms on the settlement of <i>Mytilus coruscus </i>plantigrades

Xie Jingyi; Wang Xiaoyu; Li Ju; Yang Jinlong; Liang Xiao

doi:10.12284/hyxb2023104

Volume 45 Issue 8

Aug. 2023

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Xie Jingyi，Wang Xiaoyu，Li Ju, et al. Effect of the content of colanic acid in marine bacterial biofilms on the settlement of Mytilus coruscus plantigrades[J]. Haiyang Xuebao，2023, 45(8)：96–107 doi: 10.12284/hyxb2023104

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Xie Jingyi，Wang Xiaoyu，Li Ju, et al. Effect of the content of colanic acid in marine bacterial biofilms on the settlement of Mytilus coruscus plantigrades[J]. Haiyang Xuebao，2023, 45(8)：96–107 doi: 10.12284/hyxb2023104

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Effect of the content of colanic acid in marine bacterial biofilms on the settlement of Mytilus coruscus plantigrades

doi: 10.12284/hyxb2023104

Xie Jingyi^{1, 2, 3
,},
Wang Xiaoyu^{1, 2, 3},
Li Ju^{1, 2, 3},
Yang Jinlong^{1, 2, 3},
Liang Xiao^{1, 2, 3
,
,}

1.
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
3.
Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai Ocean University, Shanghai 201306, China

Received Date: 2023-01-29
Rev Recd Date: 2023-04-17

Available Online: 2023-08-22

Publish Date: 2023-08-31

Abstract

Abstract

Colanic acid is one of the vital exopolysaccharides in biofilms, yet the effect of marine bacterial colanic acid on the settlement of invertebrates is still rarely covered. In this study, eight strains of marine bacteria isolated from natural biofilms were identified and the phylogenetic analysis was carried out, the colanic acid content and inducing ability of biofilms were also determined. Before that, the biofilm formation capacity and the extracellular products of the screened bacteria were detected. It was found that β-polysaccharide had a significant positive correlation with the settlement rate of Mytilus coruscus plantigrades (p < 0.05). The quantitative results of colanic acid content showed that among the five Gram-negative bacteria which could produce colanic acid, Shewanella marisflavi had the highest colanic acid yield of 1076.43 μg/mL, and three Gram-positive bacteria could not generate it. The results of plantigrade settlement induced by different biofilms showed that the inducing activity of marine bacterial biofilms on the settlement rate of M. coruscus was positively correlated with the content of colanic acid (p < 0.05). These findings prove that the Gram-negative bacteria with colanic acid can positively regulate the settlement of M. coruscus plantigrades, while the Gram-positive bacteria without colanic acid have no inducing activity. This study provides further insights to figure out the interaction between the chemical cues of biofilms and marine molluscs.
- biofilm,
- Mytilus coruscus,
- plantigrade,
- colanic acid,
- settlement

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References(47)

References

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