Metabolomic analysis of <i>Trachinotus ovatus</i> under flow velocity stress

Zhang Jing; Dai Jiayue; Lai Xinhao; Liu Xuxiang; Zhang Hao; Wang Xuefeng; Tang Baogui

doi:10.12284/hyxb2023060

Volume 45 Issue 5

May 2023

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Zhang Jing，Dai Jiayue，Lai Xinhao, et al. Metabolomic analysis of Trachinotus ovatus under flow velocity stress[J]. Haiyang Xuebao，2023, 45(5)：53–63 doi: 10.12284/hyxb2023060

Citation:

Zhang Jing，Dai Jiayue，Lai Xinhao, et al. Metabolomic analysis of Trachinotus ovatus under flow velocity stress[J]. Haiyang Xuebao，2023, 45(5)：53–63 doi: 10.12284/hyxb2023060

Zhang Jing，Dai Jiayue，Lai Xinhao, et al. Metabolomic analysis of Trachinotus ovatus under flow velocity stress[J]. Haiyang Xuebao，2023, 45(5)：53–63 doi: 10.12284/hyxb2023060

Citation:

Zhang Jing，Dai Jiayue，Lai Xinhao, et al. Metabolomic analysis of Trachinotus ovatus under flow velocity stress[J]. Haiyang Xuebao，2023, 45(5)：53–63 doi: 10.12284/hyxb2023060

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Metabolomic analysis of Trachinotus ovatus under flow velocity stress

doi: 10.12284/hyxb2023060

Zhang Jing^{1, 2
,},
Dai Jiayue^1
,,
Lai Xinhao¹,
Liu Xuxiang¹,
Zhang Hao¹,
Wang Xuefeng^{1, 2},
Tang Baogui^{1, 2, 3
,
,}

1.
Fishery College, Guangdong Ocean University, Zhanjiang 524088, China
2.
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China
3.
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China

Received Date: 2022-09-03
Rev Recd Date: 2022-11-17

Available Online: 2022-12-08

Publish Date: 2023-05-01

Abstract

Abstract

Flow velocity is one of the key environmental factors for deep sea aquaculture. In order to further understand the molecular regulation mechanism of golden pompano (T. ovatus) under flow velocity stress, the fishes were stressed under the static water, medium flow velocity (54 cm/s) and high flow velocity (90 cm/s), and the LC/MS metabolomics technology was used to explore the changes of endogenous metabolites in the liver to look for differential metabolites and related metabolic pathways. The results show that 51 metabolites in the medium flow velocity group have significant difference with which in the static water and are enriched in 40 pathways, mainly including galactose metabolism, gluconeogenesis, biosynthesis of unsaturated fatty acids, arachidonic acid metabolism, etc. Forty metabolites in the high flow velocity group have significant difference with which in the static water and are enriched in 22 pathways, mainly including biosynthesis of unsaturated fatty acids, pantothenate and CoA biosynthesis, lysine degradation, arachidonic acid metabolism, linoleic acid metabolism, etc. Compared with the medium flow velocity group, 31 metabolites in the high flow velocity group have significant difference and are enriched in 12 pathways, mainly including arachidonic acid metabolism, adipocytokine signaling pathway, cysteine and methionine metabolism, etc. Pathway analysis shows that both medium and high flow velocity stress promoted unsaturated fatty acid metabolism, arachidonic acid metabolism and pantothenic acid synthesis. The ability of swimming is improved to against the stress of flow velocity by strengthening metabolism. The medium flow velocity group mainly significantly affected the metabolism of galactose, cysteine and methionine. At this time, more and more energy produced by fish is used for exercise. The high flow velocity group mainly significantly affect the metabolism of L-lysine and retinoic acid, which reveals that the swimming ability of fish may be decreased and some immune functions may be triggered under the high flow velocity stress.
- Trachinotus ovatus,
- velocity stress,
- metabolome analysis

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References(35)

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