Sequencing and analysis of the complete mitochondrial genome of green abalone (<i>Haliotis fulgens</i>)

Guo Rui; Zhang Shanpi; Cai Leiming; Yang Xiaoqiang; Wang Wei; Jiang Xiaobin; Lin Qin; Lin Feng; Lin Zhelong

doi:10.12284/hyxb2023050

Volume 45 Issue 5

May 2023

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Guo Rui，Zhang Shanpi，Cai Leiming, et al. Sequencing and analysis of the complete mitochondrial genome of green abalone (Haliotis fulgens)[J]. Haiyang Xuebao，2023, 45(5)：64–78 doi: 10.12284/hyxb2023050

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Guo Rui，Zhang Shanpi，Cai Leiming, et al. Sequencing and analysis of the complete mitochondrial genome of green abalone (Haliotis fulgens)[J]. Haiyang Xuebao，2023, 45(5)：64–78 doi: 10.12284/hyxb2023050

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Sequencing and analysis of the complete mitochondrial genome of green abalone (Haliotis fulgens)

doi: 10.12284/hyxb2023050

Guo Rui^{1, 2
,},
Zhang Shanpi^{1, 2
,
,},
Cai Leiming^{1, 2},
Yang Xiaoqiang^{1, 2},
Wang Wei^{1, 2},
Jiang Xiaobin^{1, 2},
Lin Qin³,
Lin Feng³,
Lin Zhelong³

1.
Marine and Fisheries Technology Research Center, Institute of Oceanography of Fuzhou, Fuzhou 350108, China
2.
Fuzhou Ocean and Fisheries Technology Center, Fuzhou 350007, China
3.
Fujian Lianjiang Guanwu Marine Products and Development Co., Ltd., Lianjiang 350511, China

Received Date: 2022-07-19
Rev Recd Date: 2022-10-21

Available Online: 2022-11-02

Publish Date: 2023-05-01

Abstract

Abstract

To identify abalone species effectively for better management and protection of abalone germplasm resources, we have obtained the complete mitochondrial genome of juvenile green abalone (Haliotis fulgens) through high-throughput sequencing, and its sequences and structural characteristics are analyzed accordingly. The results show that the mitogenome of H. fulgens is 17 041 bp in total length and encoded 37 genes, including 13 protein coding genes, 22 transfer RNA (tRNA) genes and 2 ribosomal RNA (rRNA) genes. The 13 protein coding genes used AUG as the starting codon and UAA or UAG as the termination codon. Twenty-one tRNA genes other than tRNA-Ser^(AGN) could be folded into a typical cloverleaf structure. There is a rich A+T non-coding region between tRNA-Glu and COX3, with two hairpin structures containing palindromes in it. A phylogenetic tree was constructed based on the reported complete mitochondrial genome sequences of Haliotis genus species, and phylogenetic analysis shows that H. fulgens is clustered with H. discus hannai, H. rufescens and H. cracherodii. Comparing the domains of 13 proteins of mitogenome of H. fulgens and H. discus hannai, we found the number of transmembrane domains of ND2 or ND4 dehydrogenase subunits were different in them. Whether this is related to the difference of high temperature tolerance between them needs further study. In brief, the first acquisition and analysis of the complete mitochondrial genome of H. fulgens has enriched the abalone cellular genetic information, and provided basic data and references for abalone species classification, germplasm identification, and protection of germplasm resources of H. fulgens as well.
- Haliotis fulgens,
- mitochondrial genome,
- genome composition,
- phylogeny,
- domain

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References(68)

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