Effects of sulfide stress on blood <inline-formula><tex-math id="Z-20115510">\begin{document}${{\bf {SO}} _4^{2-}} $\end{document}</tex-math><alternatives><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="1-1sunhuimiao_Z-20115510.jpg"/><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="1-1sunhuimiao_Z-20115510.png"/></alternatives></inline-formula> concentration and <i>SULT1B1-12 </i>gene expression in <i>Sinonovacula constricta</i>

Sun Huimiao; Shen Weiliang; Chen Caifang; Lin Zhihua; Han Qingxi

doi:10.12284/hyxb2023004

Volume 45 Issue 1

Jan. 2023

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Sun Huimiao，Shen Weiliang，Chen Caifang, et al. Effects of sulfide stress on blood \scriptsize${{\rm {SO}} _4^{2-}} $ concentration and SULT1B1-12 gene expression in Sinonovacula constricta[J]. Haiyang Xuebao，2023, 45(1)：62–70 doi: 10.12284/hyxb2023004

Citation:

Sun Huimiao，Shen Weiliang，Chen Caifang, et al. Effects of sulfide stress on blood

\scriptsize${{\rm {SO}} _4^{2-}} $

concentration and SULT1B1-12 gene expression in Sinonovacula constricta[J]. Haiyang Xuebao，2023, 45(1)：62–70 doi: 10.12284/hyxb2023004

Citation:

Sun Huimiao，Shen Weiliang，Chen Caifang, et al. Effects of sulfide stress on blood

\scriptsize${{\rm {SO}} _4^{2-}} $

concentration and SULT1B1-12 gene expression in Sinonovacula constricta[J]. Haiyang Xuebao，2023, 45(1)：62–70 doi: 10.12284/hyxb2023004

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Effects of sulfide stress on blood ${{\bf {SO}} _4^{2-}} $ concentration and SULT1B1-12 gene expression in Sinonovacula constricta

doi: 10.12284/hyxb2023004

Sun Huimiao^{1, 2
,},
Shen Weiliang³,
Chen Caifang^{1
,
,},
Lin Zhihua^{1, 4},
Han Qingxi²

1.
Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
2.
School of Marine Sciences, Ningbo University, Ningbo 315823, China
3.
Ningbo Academy of Oceanology and Fishery, Ningbo 315012, China
4.
Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ninghai 315604, China

Received Date: 2022-03-25
Rev Recd Date: 2022-06-24

Available Online: 2022-11-04

Publish Date: 2023-01-09

Abstract

Abstract

As a typical dwelled tidal shellfish, Sinonovacula constricta is often exposed to sulfide-rich environment and shows strong sulfide tolerance. The cytosolic sulfotransferase 1B1 (SULT1B1) is located at downstream of the sulfur metabolism pathway, while it is a key enzyme catalyzing the sulfation reaction and plays an important role in the biotransformation of endogenous substances such as thyroid hormones (THs). In order to study the role of ScSULT1B1-12 in sulfur resistance, the sequence characteristics were analyzed by bioinformatics method. Combined with the changes of blood ${\rm {SO}} _4^{2-} $ concentration, the spatial expression and temporal expression profiles during 72 h sulfide stress (50 μmol/L, 150 μmol/L, 300 μmol/L) were studied. The full-length cDNA of ScSULT1B1-12 gene was 1 100 bp, containing an open reading frame of 897 bp, and encoding 298 amino acids. Sequence analysis showed that ScSULT1B1-12 contains four catalytic active sites (₅₆K, ₁₀₄N, ₁₀₆H, and ₁₃₄A), one PAPS binding domain (YPKSGTXW) at N terminal, and one PAPS binding and dimerization domain (RKGXXGDWKNXFTVXXE) at C terminal, indicating that it was structurally able to catalyze the sulfation reaction. Spatial expression showed that ScSULT1B1-12 was highly expressed in gills, followed by the adductor muscle and hepatopancreas. Blood ${\rm {SO}} _4^{2-} $ concentration decreased, and the expression patterns of ScSULT1B1-12 also declined with fluctuation after sulfide stress, indicating that sulfate can be further transformed to sulfated donors, and ScSULT1B1-12-mediated sulfation may be inhibited to keep THs at a certain level in S. constricta, in order to strengthen the metabolic and immune functions, and make the organism adapt the adverse environment of high sulfide.
- Sinonovacula constricta,
- ScSULT1B1-12,
- sulfide stress,
- temporal expression,
- sulfate ion

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References(37)

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