Knockout of<i> Pseudoalteromonas marina pilZ</i> gene inhibited the settlement and metamorphosis of <i>Mytilus coruscus</i>

Zhang Chi; Wang Jinsong; Yang Jinlong; Zhang Junbo; Wan Rong; Liang Xiao

doi:10.12284/hyxb2022090

Volume 44 Issue 4

Apr. 2022

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Zhang Chi，Wang Jinsong，Yang Jinlong, et al. Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus[J]. Haiyang Xuebao，2022, 44(4)：95–103 doi: 10.12284/hyxb2022090

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Zhang Chi，Wang Jinsong，Yang Jinlong, et al. Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus[J]. Haiyang Xuebao，2022, 44(4)：95–103 doi: 10.12284/hyxb2022090

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Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus

doi: 10.12284/hyxb2022090

Zhang Chi^{1, 2
,},
Wang Jinsong^{1, 2},
Yang Jinlong^{1, 2, 3},
Zhang Junbo^{4, 5},
Wan Rong^{4, 5, 6},
Liang Xiao^{1, 2
,
,}

1.
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2.
Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai 201306, China
3.
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China
4.
College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China
5.
National Engineering Research Center for Oceanic Fisheries, Shanghai 201306, China
6.
Zhoushan Branch of National Engineering Research Center for Oceanic Fisheries, Zhoushan 316014, China

Received Date: 2021-11-10
Rev Recd Date: 2021-12-27
Publish Date: 2022-04-14

Abstract

Abstract

To investigate the effect of Pseudoalteromonas marina pilZ gene knockout on the biofilm formation and its influence on the settlement and metamorphosis of Mytilus coruscus larvae, ΔpilZ was constructed by homologous recombination, and the changes in bacterial density, biofilm thickness, c-di-GMP level and extracellular polymeric substances (EPS) of ΔpilZ bacteria biofilm were analyzed, as well as the regulation of the settlement and metamorphosis of M. coruscus larvae. The results showed that compared with the wild-type strain, the biofilm formed by ΔpilZ strain significantly increased the biofilm thickness, the number of bacteria, while the contents of β-polysaccharides and proteins in EPS were decreased and the settlement and metamorphosis of M. coruscus larvae were inhibited (p<0.05). There was no significant difference in c-di-GMP level, α-polysaccharide and lipid contents (p>0.05). In conclusion, P. marina pilZ gene knockout can regulate the bacterial biofilm and the content of EPS including β-polysaccharides and proteins contents, then inhibit the settlement and metamorphosis of M. coruscus larvae.
- pilZ,
- biofilm,
- Mytilus coruscus,
- settlement and metamorphosis,
- extracellular polymeric substances

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References(43)

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