The association of single nucleotide polymorphisms of the glycogen phosphorylase gene with glycogen content in the razor clam <i>Sinonovacula constricta</i>

Chen Yanyuan; Lin Zhihua; Liu Sheng; Yao Hanhan; Dong Yinghui

doi:10.12284/hyxb2022068

Volume 44 Issue 2

Feb. 2022

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Chen Yanyuan，Lin Zhihua，Liu Sheng, et al. The association of single nucleotide polymorphisms of the glycogen phosphorylase gene with glycogen content in the razor clam Sinonovacula constricta[J]. Haiyang Xuebao，2022, 44(2)：77–83 doi: 10.12284/hyxb2022068

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Chen Yanyuan，Lin Zhihua，Liu Sheng, et al. The association of single nucleotide polymorphisms of the glycogen phosphorylase gene with glycogen content in the razor clam Sinonovacula constricta[J]. Haiyang Xuebao，2022, 44(2)：77–83 doi: 10.12284/hyxb2022068

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The association of single nucleotide polymorphisms of the glycogen phosphorylase gene with glycogen content in the razor clam Sinonovacula constricta

doi: 10.12284/hyxb2022068

Chen Yanyuan^{1, 2
,},
Lin Zhihua^{1, 3},
Liu Sheng³,
Yao Hanhan^{1
,
,},
Dong Yinghui¹

1.
Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
2.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
3.
Ninghai Marine Biological Seed Industry Research Institute, Zhejiang Wanli University, Ninghai 315604, China

Received Date: 2021-06-07
Rev Recd Date: 2021-11-21

Available Online: 2021-12-25

Publish Date: 2022-02-01

Abstract

Abstract

To clarify the relationship between the glycogen phosphorylase gene (Sc-GPH) and glycogen content in Sinonovacula constricta, the full-length cDNA sequence of Sc-GPH is obtained using RACE technology, its expression pattern in different tissues, different months are analyzed, and the SNP loci of Sc-GPH associated with glycogen content are explored. The full-length cDNA of Sc-GPH gene is 3 963 bp and the ORF is 2 541 bp, encoding 846 amino acids. The multiple alignment of amino acid sequences and phylogenetic tree show that S. constricta are closely related to shellfish such as Pecten maximus, Mizuhopecten yessoens, Crassostrea gigas, but farther to mammals, arthropodas and insects. The results of real-time quantitative PCR show that Sc-GPH gene is expressed in eight tissues, with the highest expression in mantle and foot, suggesting they are related to glycogen storage capacity. In different months, the expression levels of Sc-GPH in the mantle and foot is the highest in August, at which time the glycogen content decreased, indicating that the expression of Sc-GPH seems to be driven by reproductive cycle of S. constricta. In “YL1” population, four SNPs related to glycogen content are screened in the coding region of Sc-GPH gene, and c.930T>C is further verified in Taizhou wild population, which provides a candidate marker for molecular marker assisted breeding of high glycogen S. constricta varieties.
- Sinonovacula constricta,
- glycogen phosphorylase,
- glycogen content,
- SNP,
- correlation analysis

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References(29)

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