Physiological responses of juvenile <i>Apostichopus japonicus</i> (Selenka) to collaborative stress of high temperature and low salinity: Growth and induced heat shock protein gene expression

Yu Shanshan; Li Xueyu; He Zhenping; Wang Qinglin; Sun Guiqing; Fu Zhong

doi:10.12284/hyxb2022058

Volume 44 Issue 2

Feb. 2022

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Yu Shanshan，Li Xueyu，He Zhenping, et al. Physiological responses of juvenile Apostichopus japonicus (Selenka) to collaborative stress of high temperature and low salinity: Growth and induced heat shock protein gene expression[J]. Haiyang Xuebao，2022, 44(2)：94–101 doi: 10.12284/hyxb2022058

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Yu Shanshan，Li Xueyu，He Zhenping, et al. Physiological responses of juvenile Apostichopus japonicus (Selenka) to collaborative stress of high temperature and low salinity: Growth and induced heat shock protein gene expression[J]. Haiyang Xuebao，2022, 44(2)：94–101 doi: 10.12284/hyxb2022058

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Physiological responses of juvenile Apostichopus japonicus (Selenka) to collaborative stress of high temperature and low salinity: Growth and induced heat shock protein gene expression

doi: 10.12284/hyxb2022058

Yu Shanshan^{1, 2, 3
,},
Li Xueyu^{1, 3},
He Zhenping¹,
Wang Qinglin^{1, 2, 3, 4
,
,},
Sun Guiqing^{4
,
,},
Fu Zhong⁵

1.
College of Marine Resources & Environment, Hebei Normal University of Science & Technology, Qinhuangdao 066004, China
2.
Hebei Key Laboratory for Ocean Dynamics, Resources and Environments, Qinhuangdao 066004, China
3.
Qinhuangdao Key Laboratory of Marine Habitat and Resources, Qinhuangdao 066004, China
4.
Marine Living Resources and Environment Key Laboratory of Hebei Province, Qinhuangdao 066004, China
5.
Ocean and Fisheries Science Research Institute of Hebei Province (Marine Fishery Ecological Environment Monitoring Station of Hebei Province), Qinghuangdao 066004, China)

Received Date: 2021-08-31
Rev Recd Date: 2021-10-15

Available Online: 2021-12-21

Publish Date: 2022-02-01

Abstract

Abstract

Under the background of global climate change, the continuous high temperature and low salinity caused by extreme short-time heavy rainfall in summer brings severe challenges to pond culture of Apostichopus japonicus. In order to study the physiological responses of A. japonicus to high temperature and low salinity, the effects of collaborative stress of high temperature and low salt on growth and induced hsp70 and hsp90 expression of juvenile A. japonicus were analyzed in this paper. Four temperature gradients (16℃, 20℃, 24℃, and 28℃) and three salinity gradients (22, 27, and 32) were set, and there were a total of 12 different stress groups. After a period of 40 days stress, the weight gain of juvenile A. japonicus decreased with the increase of temperature and the decrease of salinity, there was negative growth in the high temperature and low salinity group. The expression of induced hsp70 and hsp90 was increased after a long period of stress, thus enhancing the resistance of juveniles to extreme weather. Meanwhile, the expression of induced hsps of juveniles treated with salinity 22 was lower than that in the condition of salinity 27. The results of two-factor ANOVA showed that there was no significant interaction between high temperature and low salinity on specific growth rate and induced hsp70 and hsp90 expression, and salinity had no significant effect on induced hsp70 and hsp90 expression. Therefore, compared with low salinity, high temperature had a greater impact on juvenile A. japonicus, which could be used as the selection pressure in A. japonicus breeding. During the high temperature period, effective measures should be taken to reduce the sea temperature and prevent the stratification of aquaculture water caused by extreme weather. This study enriched the physiological and ecological theories of A. japonicus, provided ideas for the breeding of A. japonicus, and provided certain theoretical basis for guiding the production and practice of A. japonicus culture under extreme weather.
- high temperature,
- low salinity,
- collaborative stress,
- Apostichopus japonicus,
- growth,
- heat shock protein

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References(46)

References

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