Identification of genes related to blind side hypermelanosis of <i>Cynoglossus semilaevis </i>based on skin transcriptome sequencing

Zhao Na; He Xiaoxu; Jia Lei; Zhu Chunhua; Zhang Bo

doi:10.12284/hyxb2022044

Volume 44 Issue 2

Feb. 2022

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Zhao Na，He Xiaoxu，Jia Lei, et al. Identification of genes related to blind side hypermelanosis of Cynoglossus semilaevis based on skin transcriptome sequencing[J]. Haiyang Xuebao，2022, 44(2)：57–66 doi: 10.12284/hyxb2022044

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Zhao Na，He Xiaoxu，Jia Lei, et al. Identification of genes related to blind side hypermelanosis of Cynoglossus semilaevis based on skin transcriptome sequencing[J]. Haiyang Xuebao，2022, 44(2)：57–66 doi: 10.12284/hyxb2022044

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Identification of genes related to blind side hypermelanosis of Cynoglossus semilaevis based on skin transcriptome sequencing

doi: 10.12284/hyxb2022044

Zhao Na^{1, 3
,},
He Xiaoxu^{2, 4},
Jia Lei²,
Zhu Chunhua^{1
,
,},
Zhang Bo^{1, 2
,}

1.
College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China
2.
Tianjin Fisheries Research Institute, Tianjin 300457, China
3.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
4.
Tianjin Agricultural University, Tianjin 300384, China

Received Date: 2021-08-19
Rev Recd Date: 2021-11-02

Available Online: 2021-12-06

Publish Date: 2022-02-01

Abstract

Abstract

Cynoglossus semilaevis as a representative species of Pleuronectiformes, is an important marine economic fish in Chinese coastal areas. The abnormal body color of C. semilaevis has been perplexing the practitioners for a long time. There are many reasons leading to abnormal body color, among which the genetic factors are thought to be the most fundamental reasons. The present researches on hypermelanosis of the blind side of C. semilaevis mainly focus on environment, nutrition, physiology, cloning of known pigmental genes and so on, while the digging of new funtional genes is still lacking. In this study, skin samples with different colors of C. semilaevis are selected for transcriptome sequencing. Through GO and KEGG functional enrichment and comparative analysis of different genes, differential expressed genes are screened in six melanogenesis-related KEGG pathways and the top ten genes are verified by qPCR. In this study, we find five functional genes with significant changes in the hypermelanotic skin on the blind side of C. semilaevis, which refer to txndc, alox15b, ptgs2, ptgis, and atp1a2a (p<0.05). The expression levels of txndc, alox15b, ptgs2, and ptgis genes in the melanization group are higher than those in the control group. In terms of function, three of these five genes are related to arachidonic acid (AA) to some extent. This provides theoretical support for the hypothesis that nutritional regulation related to unsaturated fatty acids may be involved in the molecular mechanism of abnormal body color in C. semilaevis.
- skin,
- Cynoglossus semilaevis,
- hypermelanosis,
- arachidonic acid,
- transcriptome

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References(66)

References

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