Histological observation on gonadal differentiation and first annual gonadal development of cobia (<i>Rachycentron canadum</i>)

Kuang Jiehua; Chen Gang; Ma Qian; Mao Feifan; Zhou Qiling; Huang Jiansheng; Shi Gang; Zhang Jiandong

doi:10.12284/hyxb2021126

Volume 43 Issue 8

Aug. 2021

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Kuang Jiehua，Chen Gang，Ma Qian, et al. Histological observation on gonadal differentiation and first annual gonadal development of cobia (Rachycentron canadum)[J]. Haiyang Xuebao，2021, 43(8)：128–138 doi: 10.12284/hyxb2021126

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Kuang Jiehua，Chen Gang，Ma Qian, et al. Histological observation on gonadal differentiation and first annual gonadal development of cobia (Rachycentron canadum)[J]. Haiyang Xuebao，2021, 43(8)：128–138 doi: 10.12284/hyxb2021126

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Histological observation on gonadal differentiation and first annual gonadal development of cobia (Rachycentron canadum)

doi: 10.12284/hyxb2021126

Kuang Jiehua^1
,,
Chen Gang^{1, 2
,
,},
Ma Qian^{1, 2
,
,},
Mao Feifan¹,
Zhou Qiling¹,
Huang Jiansheng^{1, 2},
Shi Gang¹,
Zhang Jiandong^{1, 2}

1.
College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China
2.
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China

Received Date: 2021-03-13
Rev Recd Date: 2021-04-16

Available Online: 2021-05-28

Publish Date: 2021-08-25

Abstract

Abstract

In this study, histological observation of gonadogenesis, gonadal differentiation and first annual gonadal development in cobia (Rachycentron canadum) was performed based on paraffin section and H.E. staining method. The results of histological observations showed that the primordial germ cells (PGCs) arrived at the genital ridge at 7 dph (days post hatching), then the somatic cells began to aggregate around the PGCs. At 15 dph, the PGCs were enclosed entirely by somatic cells and formed the primary gonad. Two types of primary gonads with noticeable histological differences could be observed at 34 dph. The presence of clusters of oogonia characterized a part of primary gonads. The other primary gonads showed a narrower cross-section with fewer germ cells, which were presumed to be undifferentiated testis. Similar to other fishes, ovarian differentiation of cobia was anterior to testicular differentiation. The anatomical indication of ovarian differentiation was the ovarian cavity formation, which occurred at 44 dph. The cytological differentiation of testis began at about 50 dph and was characterized by the presence of spermatogonial acinar-clusters. The first annual development of testes could be divided into five stages (I, II, III, IV, V), while the development of the ovaries was divided into three stages (I, II, III). Testes and ovaries were both developed to early Stage I at 60 dph. All of the testes developed to Stage II at 90 dph; the ovaries were still at Stage I. More than half of the testes developed to Stage III and part of the ovaries developed to Stage II at 120 dph. The testes were mainly developed to Stage III at 150 dph; most of the ovaries were at Stage II. The testes were still at stage III, and the ovaries were developed to Stage II at 185 dph. Most of the testes developed to Stage IV at 210 dph, but the ovaries were still at Stage II. At 360 dph, the testes entered Stage V, and most of the ovaries developed to Stage III. These findings could clarify gonadal development characteristics and provide a theoretical basis for the artificial breeding of cobia.
- Rachycentron canadum,
- primordial germ cells,
- gonadal differentiation,
- gonadal development,
- histological observation

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References(38)

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