Effects of low temperature stress on the expression of genes related to lipid metabolism of juvenile cobia, <i>Rachycentron canadum</i>

Cai Runjia; Zhang Jing; Huang Jiansheng; Shi Gang; Pan Chuanhao; Xie Ruitao; Chen Gang; Zhang Jiandong; Wang Zhongliang; Tang Baogui

doi:10.12284/hyxb2021122

Volume 43 Issue 11

Dec. 2021

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Cai Runjia，Zhang Jing，Huang Jiansheng, et al. Effects of low temperature stress on the expression of genes related to lipid metabolism of juvenile cobia, Rachycentron canadum[J]. Haiyang Xuebao，2021, 43(11)：116–122 doi: 10.12284/hyxb2021122

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Cai Runjia，Zhang Jing，Huang Jiansheng, et al. Effects of low temperature stress on the expression of genes related to lipid metabolism of juvenile cobia, Rachycentron canadum [J]. Haiyang Xuebao，2021, 43(11)：116–122 doi: 10.12284/hyxb2021122

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Effects of low temperature stress on the expression of genes related to lipid metabolism of juvenile cobia, Rachycentron canadum

doi: 10.12284/hyxb2021122

1.
Fishery College, Guangdong Ocean University, Zhanjiang 524088, China
2.
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China
3.
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China

Received Date: 2021-01-01
Rev Recd Date: 2021-03-30

Available Online: 2021-05-08

Publish Date: 2021-12-31

Abstract

Abstract

In order to explore the effects of low temperature stress on the expression of genes related to lipid synthesis and catabolism in cobia (Rachycentron canadum), the experiment set up a normal temperature group (30.5±1.0)°C and a low temperature group (20.0±0.5)°C, and used real-time fluorescent quantitative PCR (qRT-PCR) to analyze the expression levels of 5 target genes in liver, muscle and intraperitoneal fat (IPF). The results showed that at 1 d, the expression of carnitine palmitoyl transferase-1 and hormone-sensitive lipase genes of liver, carnitine palmitoyl transferase-1, hormone-sensitive lipase and monoacylglycerol lipase genes of muscle were up-regulated (p<0.05), acetyl-CoA carboxylase and fatty acid synthase genes of liver, muscle and 5 lipid metabolism related genes of IPF were significantly down-regulated (p<0.05); at 4 d, the expression of carnitine palmitoyl transferase-1, hormone-sensitive lipase and monoacylglycerol lipase genes of liver, and hormone-sensitive lipase, monoacylglycerol lipase, acetyl-CoA carboxylase, fatty acid synthase genes of muscle and carnitine palmitoyl transferase-1, hormone-sensitive lipase, monoacylglycerol lipase, acetyl-CoA carboxylase genes of IPF were up-regulated (p<0.05), acetyl-CoA carboxylase and fatty acid synthase gene of liver were down-regulated (p<0.05); at 7 d, the expressions of carnitine palmitoyl transferase-1, hormone-sensitive lipase, monoacylglycerol lipase, acetyl-CoA carboxylase genes of liver and IPF, and hormone-sensitive lipase, monoacylglycerol lipase, acetyl-CoA carboxylase genes of muscle were up-regulated (p<0.05), carnitine palmitoyl transferase-1 gene of muscle and fatty acid synthase genes of liver were down-regulated (p<0.05). The results showed that cobia responded to low temperature stress by inhibiting lipid synthesis and metabolism, promoting lipid hydrolysis in the liver and muscle, and inhibiting the lipid hydrolysis of IPF in the early stage of low temperature stress; in the late period of low temperature stress, cobia lipid synthesis and catabolism were significantly increased, and the main tissue that used fatty acids to provide energy was transformed from the liver and muscle to liver and IPF.
- low temperature stress,
- lipid metabolism,
- gene expression,
- Rachycentron canadum,
- real-time qRT-PCR

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