Ultra-fast energy transfer process of recombinant allophycocyanin

Zhen Zhanghe; Zhu Ruidan; Qin Song; Chen Hailong; Pu Yang; Weng Yuxiang; Li Wenjun

doi:10.12284/hyxb2021120

Volume 43 Issue 9

Sep. 2021

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Zhen Zhanghe，Zhu Ruidan，Qin Song, et al. Ultra-fast energy transfer process of recombinant allophycocyanin[J]. Haiyang Xuebao，2021, 43(9)：115–125 doi: 10.12284/hyxb2021120

Citation:

Zhen Zhanghe，Zhu Ruidan，Qin Song, et al. Ultra-fast energy transfer process of recombinant allophycocyanin[J]. Haiyang Xuebao，2021, 43(9)：115–125 doi: 10.12284/hyxb2021120

Citation:

Zhen Zhanghe，Zhu Ruidan，Qin Song, et al. Ultra-fast energy transfer process of recombinant allophycocyanin[J]. Haiyang Xuebao，2021, 43(9)：115–125 doi: 10.12284/hyxb2021120

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Ultra-fast energy transfer process of recombinant allophycocyanin

doi: 10.12284/hyxb2021120

Zhen Zhanghe^1
,,
Zhu Ruidan^{2, 3
,},
Qin Song^{1, 4},
Chen Hailong³,
Pu Yang⁵,
Weng Yuxiang^{2, 3
,
,},
Li Wenjun^{1, 4
,
,}

1.
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China
3.
Beijing National Laboratory for Condensed Matter Physics/Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
4.
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
5.
School of Agriculture, Ludong University, Yantai 264025, China

Received Date: 2021-02-19
Rev Recd Date: 2021-04-13

Available Online: 2021-05-28

Publish Date: 2021-09-25

Abstract

Abstract

Phycobilisomes are photosynthetic light-harvesting antenna complexes unique to red algae and cyanobacteria. Allophycocyanin (APC) is the main component of the core structure of phycobilisomes. In this experiment, recombinant allophycocyanin (rAPC) was used as the material, and the structure conformation and energy transfer process of rAPC were studied through steady-state spectroscopy, circular dichroic spectroscopy, and ultrafast time-resolved spectroscopy. The results show that rAPC can maintain the same spectral characteristics and active conformation as natural APC under the test conditions; after rAPC monomers are assembled into trimer, the results confirmed that α84PCB and β84PCB can form an exciton pigment pair which can improve the energy transfer efficiency of the rAPC trimer through exciton splitting; ultrafast time-resolved spectroscopy results show that the energy transfer time from 620 nm to 650 nm is 300−600 fs, and there is also a 19 fs exciton state electronic decoherence process. These results provide a data basis for revealing the efficient energy transfer mechanism of phycobilisomes.
- allophycocyanin,
- recombination and heterologous expression,
- energy transfer,
- steady-state spectrum,
- transient spectrum

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References(49)

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