Effect of salinity stress on the antioxidant enzymes, non-specific immune enzymes, and Na+/K+-ATPase activities in Larimichthys polyactis

Wang Mengjie; Chu Tianqi; Liu Feng; Zhan Wei; Lou Bao; Xu Wantu

doi:10.12284/hyxb2021038

Volume 43 Issue 2

Mar. 2021

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Wang Mengjie，Chu Tianqi，Liu Feng, et al. Effect of salinity stress on the antioxidant enzymes, non-specific immune enzymes, and Na+/K+-ATPase activities in Larimichthys polyactis[J]. Haiyang Xuebao，2021, 43(2)：59–66 doi: 10.12284/hyxb2021038

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Wang Mengjie，Chu Tianqi，Liu Feng, et al. Effect of salinity stress on the antioxidant enzymes, non-specific immune enzymes, and Na⁺/K⁺-ATPase activities in Larimichthys polyactis [J]. Haiyang Xuebao，2021, 43(2)：59–66 doi: 10.12284/hyxb2021038

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Effect of salinity stress on the antioxidant enzymes, non-specific immune enzymes, and Na⁺/K⁺-ATPase activities in Larimichthys polyactis

doi: 10.12284/hyxb2021038

Wang Mengjie^1
,,
Chu Tianqi¹,
Liu Feng^{2
,
,},
Zhan Wei²,
Lou Bao^{2
,
,},
Xu Wantu³

1.
School of Fisheries, Zhejiang Ocean University, Zhoushan 316022, China
2.
Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3.
Xiangshan Gangwan Aquatic Seeds Co., Ltd., Ningbo 315700, China

Received Date: 2020-06-05
Rev Recd Date: 2020-09-21

Available Online: 2021-01-23

Publish Date: 2021-03-02

Abstract

Abstract

In order to study the physiological effects of different salinity stress on Larimichthys polyactis, the four-month-old L. polyactis (body weight (12.6±3.1) g) cultured in natural seawater with salinity of 22.1 were applied to salinity stress in seawater salinity of 5 (called as low-salinity group) and 34.5 (called as high-salinity group), and natural seawater was used as a control group. After 10 days of acute salinity stress experimental treatment, the activities of superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (AKP) and acid phosphatase (ACP) in the liver and Na⁺/K⁺-ATPase in gills and kidneys were measured and analyzed. The results showed that under acute salinity stress, the activity of antioxidant enzymes (SOD and CAT) in the liver were increased. Among them, the activities of SOD in the low-salinity group and CAT in the high-salinity group were significantly higher than those in the control group (p<0.05). Under different salinity conditions, AKP and ACP showed an opposite trend of change, i.e. AKP activity gradually increased with increasing salinity, while ACP activity gradually decreased. Additionally, Na⁺/K⁺-ATPase activity in gills was the lowest in the low salinity group, and the activity of which in the kidney in the high salinity group was significantly higher than that of the control group (p<0.05). The above research results indicate that juveniles of L. polyactis can still survive normally when the salinity drops to 5, without obvious abnormality. And different salinity stress can significantly affect the non-specific immune enzymes activities of the liver, and the Na⁺/K⁺-ATPase activity of the gills and kidneys in L. polyactis. In other word, the tissues of liver, gill and kidney play an important role during the process of L. polyactis adapting to changes in salinity. The research results also provide a reference for the cultivation of L. polyactis in high-saline or brackish water areas.
- salinity stress,
- Larimichthys polyactis,
- non-specific immune enzyme,
- Na⁺/K⁺-ATPase

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References(40)

References

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