Isolation, identification and polyethylene-degrading characteristics of <i>Bacillus</i> LC-2

Jiang Tingting; Ding Huiping; Feng Lijuan; Zhang Dahai; Li Ling; Liu Yandong; Li Xianguo

doi:10.12284/hyxb2021036

Volume 43 Issue 2

Mar. 2021

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Jiang Tingting，Ding Huiping，Feng Lijuan, et al. Isolation, identification and polyethylene-degrading characteristics of Bacillus LC-2[J]. Haiyang Xuebao，2021, 43(2)：9–15 doi: 10.12284/hyxb2021036

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Jiang Tingting，Ding Huiping，Feng Lijuan, et al. Isolation, identification and polyethylene-degrading characteristics of Bacillus LC-2[J]. Haiyang Xuebao，2021, 43(2)：9–15 doi: 10.12284/hyxb2021036

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Isolation, identification and polyethylene-degrading characteristics of Bacillus LC-2

doi: 10.12284/hyxb2021036

Jiang Tingting^{1, 2
,},
Ding Huiping^{1, 2},
Feng Lijuan^{1, 2},
Zhang Dahai^{1, 2},
Li Ling^{1, 2},
Liu Yandong^{1, 2},
Li Xianguo^{1, 2
,
,}

1.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
2.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao 266100, China

Received Date: 2020-08-30
Rev Recd Date: 2020-10-26

Available Online: 2021-01-21

Publish Date: 2021-03-02

Abstract

Abstract

Plastics accumulate in the environment and gradually break into microplastics (MPs, size below 5 mm). It poses a serious threat to the environment and human health. In this study, a strain LC-2 capable of degrading polyethylene (PE) was isolated from plastic films collected from the Licun Estuary of Qingdao. It was identified as Bacillus aquimaris by using a combination of molecular biological technique with morphological, physiological and biochemical characteristics. After the strain was isolated, it was cultured in a liquid medium for 28 days with PE as the sole carbon source. The polyethylene was subsequently separated and examined with a scanning electron microscopy, a contact angle test, a thermogravimetric analysis and a Fourier transform infrared spectrum analysis. The results showed that the weight loss of PE was about 9% after the degradation, with apparent morphologic changes on the surface of PE. It was also showed that the hydrophobicity of PE became smaller, with surface oxidation to produce −C=O functional groups. These evidences are sufficient to prove that the strain LC-2 can degrade PE.
- polyethylene,
- plastic,
- degradation,
- Bacillus aquimaris

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References(28)

References

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