Genetic diversity of <i>Parasesarma affine</i> from the South China Sea based on mitochondrial DNA COⅠ gene

Yang Mingliu; Xu Yan; Gao Tingwei; Pan Hongping; Wu Bin; Yan Bing

doi:10.12284/hyxb2021026

Volume 43 Issue 2

Mar. 2021

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Yang Mingliu，Xu Yan，Gao Tingwei, et al. Genetic diversity of Parasesarma affine from the South China Sea based on mitochondrial DNA COⅠ gene[J]. Haiyang Xuebao，2021, 43(2)：105–115 doi: 10.12284/hyxb2021026

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Yang Mingliu，Xu Yan，Gao Tingwei, et al. Genetic diversity of Parasesarma affine from the South China Sea based on mitochondrial DNA COⅠ gene[J]. Haiyang Xuebao，2021, 43(2)：105–115 doi: 10.12284/hyxb2021026

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Genetic diversity of Parasesarma affine from the South China Sea based on mitochondrial DNA COⅠ gene

doi: 10.12284/hyxb2021026

Yang Mingliu^1
,,
Xu Yan^{1, 2},
Gao Tingwei¹,
Pan Hongping²,
Wu Bin¹,
Yan Bing^{1
,
,}

1.
Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai 536007, China
2.
College of Animal Science and Technology, Guangxi University, Nanning 530005, China

Received Date: 2020-04-23
Rev Recd Date: 2020-09-28

Available Online: 2020-12-25

Publish Date: 2021-03-02

Abstract

Abstract

In this study, the population genetic diversity and structure of Parasesarma affine in the South China Sea were analyzed based on 222 individuals of twelve populations using Cytochrome Oxidase Ⅰ (COⅠ) sequence. A 612 bp fragment of COⅠ gene were sequenced, from which 34 polymorphic sites were tested and 40 haplotypes were defined. The most frequent haplotype was Hap2 with the highest frequency of 69.81%, which was shared in all twelve populations. Total haplotype diversity (H_d) and nucleotide diversity (P_i) were 0.508 9, 0.001 126, respectively, which represented a moderate level of haplotype diversity and a low level of nucleotide diversity. No clustering corresponding to sampling localities was found in neighbor-joining tree and haplotype network. The genetic distance ranged from 0.000 36 to 0.001 73 within populations and from 0.000 48 to 0.001 72 between populations. Genetic fixation index (F_st) and analysis of molecular variance (AMOVA) indicated that the genetic variance mainly came from individuals within populations, and a low level of genetic differentiation among twelve populations. Both neutral test and mismatch-distribution analysis implied that the populations of P. affine had a recent population expansion event that occurred in about 51 000 years ago in the late Pleistocene epoch. In summary, the longer time of the planktonic larval phase and the lack of evident geographical barriers in the marine realm might be major reasons for that P. affine could carry out extensive gene flow and possessed a low level of genetic differentiation among all twelve populations. In addition, the severe climate change of pleistocene epoch might also have important effects on the genetic structure and patterns of distribution of P. affine populations. The research results could provide a theoretical basis for the conservation and utilization of P. affine.
- Parasesarma affine,
- COⅠ gene,
- genetic diversity,
- genetic structure

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References(47)

References

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