Transcriptome analysis provides insights into the response of coelomocytes in polian vesicle and coelomic cavity of sea cucumber <i>Apostichopus japonicus</i> to evisceration

Shi Weibo; Wang Yi’nan; Wang Haowen; Ren Yuan; Wang Qingkui; Li Qiang

doi:10.12284/hyxb2021016

Volume 43 Issue 2

Mar. 2021

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Shi Weibo，Wang Yi’nan，Wang Haowen, et al. Transcriptome analysis provides insights into the response of coelomocytes in polian vesicle and coelomic cavity of sea cucumber Apostichopus japonicus to evisceration[J]. Haiyang Xuebao，2021, 43(2)：116–125 doi: 10.12284/hyxb2021016

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Shi Weibo，Wang Yi’nan，Wang Haowen, et al. Transcriptome analysis provides insights into the response of coelomocytes in polian vesicle and coelomic cavity of sea cucumber Apostichopus japonicus to evisceration[J]. Haiyang Xuebao，2021, 43(2)：116–125 doi: 10.12284/hyxb2021016

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Transcriptome analysis provides insights into the response of coelomocytes in polian vesicle and coelomic cavity of sea cucumber Apostichopus japonicus to evisceration

doi: 10.12284/hyxb2021016

1.
College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China
2.
College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China
3.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China

Received Date: 2020-05-23
Rev Recd Date: 2020-08-30

Available Online: 2021-01-21

Publish Date: 2021-03-02

Abstract

Abstract

Coelomocytes in Apostichopus japonicus, present in coelomic fluid and water-vascular system, are considered to participate in a variety of biological functions including nutrition transport, metabolism and immunity. In the process of evisceration, coelomocytes in coelom are nearly exhausted and then recovered in a short period. The polian vesicle, as the only remained internal organ after evisceration, shows positive response that coelomocytes within it increased rapidly. Coelomocytes in coelom are nearly exhausted after evisceration, and then recovered quickly. To further investigate the function and significance of the rapid increase of coelomocytes in the early stage of regeneration after evisceration, transcriptome sequencing was performed for coelomocytes in polian vesicle and coelom of A. japonicus at 6 h after evisceration and pre-evisceration, respectively. The gene expression differences of coelomocytes in polian vesicle and coelom after evisceration were analyzed compared to those of healthy A. japonicus. These results showed that 267 genes were differentially expressed in coelomocytes of polian vesicle at 6 h after evisceration, and most of these genes were enriched into the enzyme catalytic activity subclasses according to GO functional annotation and glycine, serine and threonine metabolism pathway according to KEGG pathways annotation, respectively. Additionally, 922 differential genes were significantly expressed in coelomocytes of coelom at 6 h after evisceration, and these genes were enriched into cell adhesion and biological adhesion subclasses according to GO functional annotation and ECM-receptor interaction, TGF-β signaling pathway, FoxO signaling pathway according to KEGG pathways annotation, respectively. The results provide an important basis for further functional research and the regeneration mechanism of coelomocytes after evisceration in A. japonicus.
- Apostichopus japonicus,
- coelomocytes,
- polian vesicle,
- evisceration,
- transcriptome

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References(28)

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