不同状态浒苔对东海原甲藻生长的影响

林锟; 杨雪; 谭丽菊; 王江涛

doi:10.3969/j.issn.0253-4193.2020.08.008

不同状态浒苔对东海原甲藻生长的影响

doi: 10.3969/j.issn.0253-4193.2020.08.008

林锟^1,,
杨雪¹,
谭丽菊¹,
王江涛^1, ,

中国海洋大学海洋化学理论与工程技术教育部重点实验室，山东青岛 266100

基金项目: 国家自然科学基金（41876078）；国家重点研发计划（2016YFC1402101）；中央高校基本科研业务费资助（201713059）。

详细信息

作者简介:
林锟（1996-），男，山东省威海市人，主要从事海洋污染生态化学研究。E-mail：Linkouc@163.com

通讯作者:
王江涛（1967-），男，河北省承德市人，教授，主要从事海洋化学和生态学研究。E-mail：jtwang@ouc.edu.cn

中图分类号: P714⁺.5；Q949.2
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- 被引次数: 0
出版历程
- 收稿日期: 2019-09-30
- 修回日期: 2020-05-13
- 网络出版日期: 2020-11-18
- 刊出日期: 2020-08-25

Effects of Ulva prolifera in different states on the growth of Prorocentrum donghaiense

Lin Kun^1
,,
Yang Xue¹,
Tan Liju¹,
Wang Jiangtao^{1
, ,}

Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education, Ocean University of China, Qingdao 266100, China

摘要

摘要: 近年来富营养化等海洋污染现象日益加重，我国近海每年都会暴发浒苔绿潮和微藻赤潮现象。本文在实验室模拟条件下，研究了不同状态不同含量的浒苔（Ulva prolifera），即浒苔新鲜藻体、浒苔培养液和浒苔干粉末与东海原甲藻（Prorocentrum donghaiense）共培养时产生的竞争抑制作用。结果表明，浒苔新鲜藻体、浒苔培养液和浒苔干粉末在初始质量含量大于1 g/L的条件下都会对东海原甲藻产生抑制作用，浒苔干粉末的抑制强度远远超过浒苔培养滤液和浒苔新鲜藻体。在二者共培养时，出现了“低促高抑”的现象，即浒苔含量较低时会促进东海原甲藻的生长，而高含量的浒苔则抑制东海原甲藻的生长，且抑制作用随浒苔含量的升高而增大。浒苔对东海原甲藻的这种抑制作用，一方面可能来自于营养物质的竞争和消耗，另一方面可能来自于浒苔释放的化感物质对东海原甲藻产生的影响。利用浒苔对东海原甲藻的抑制作用，可为东海原甲藻赤潮的生物防治提供一种思路与方法。
- 浒苔 /
- 东海原甲藻 /
- 竞争作用 /
- 抑制率
Abstract: In recent years, green tide of Ulva prolifera and red tide of microalgae occurred frequently with increasing marine pollution such as eutrophication in Chinese coastal waters. In this paper, the competitive inhibitory effects of U. prolifera fresh algae, U. prolifera culture filtrate and U. prolifera dry powder in different contents on Prorocentrum donghaiense were studied under laboratory simulation conditions. U. prolifera fresh algae, U. prolifera culture filtrate and U. prolifera dry powder all had inhibitory effects on P. donghaiense under the condition that the initial mass content was greater than 1 g/L, and the inhibitory intensity of U. prolifera dry powder was much higher than U. prolifera culture filtrate and U. prolifera fresh algae. In the co-culture groups, the phenomenon of “low promotion and high inhibition” appeared. Low content of U. prolifera promoted the growth of P. donghaiense, and high content of U. prolifera inhibited the growth of P. donghaiense. In addition, the inhibitory effects increased with the increasing content of U. prolifera. This inhibitory effects of U. prolifera on P. donghaiense may come from the competition and consumption of nutrients. In addition, the effects may also come from the influence of allelopathic substances released by U. prolifera on P. donghaiense. The inhibitory effects of U. prolifera on P. donghaiense can provide ideas and methods for the biological control of red tide.
- Ulva prolifera /
- Prorocentrum donghaiense /
- competition /
- inhibition rate

HTML全文

图 1 东海原甲藻与不同含量新鲜浒苔共培养的生长曲线

**表示与对照组的显著性差异(p<0.01)

Fig. 1 Growth curves of P. donghaiense in co-cultures with different contents of fresh U. prolifera

**indicates significant differences relative to the control (p<0.01)

下载: 全尺寸图片幻灯片

图 2 不同含量新鲜浒苔共培养对东海原甲藻生长抑制率（第8天）

Fig. 2 The growth inhibition rate of P. donghaiense in co-cultures with different contents of fresh U. prolifera (the 8th day)

下载: 全尺寸图片幻灯片

图 3 东海原甲藻在不同含量浒苔滤液培养中的生长曲线

*（p<0.05）和**（p<0.01）表示与对照组的显著性差异

Fig. 3 Growth curves of P. donghaiense in different contents of U. prolifera filtrate culture

*(p<0.05) and **(p<0.01) indicate significant differences relative to the control

下载: 全尺寸图片幻灯片

图 4 实验末期15 g/L浒苔滤液培养（5−1，5−2）和对照组（0−1，0−2）中的东海原甲藻的生长状况

Fig. 4 Growth of P. donghaiense in 15 g/L U. prolifera filtrate culture (5−1，5−2) and control group (0−1，0−2) at the end of the experiment

下载: 全尺寸图片幻灯片

图 5 不同含量浒苔滤液培养对东海原甲藻生长抑制率（第8天）

Fig. 5 The growth inhibition rate in different contents of U. prolifera filtrate culture on P. donghaiense (the 8th day)

下载: 全尺寸图片幻灯片

图 6 东海原甲藻与不同含量浒苔干粉末共培养的生长曲线

*（p<0.05）和**（p<0.01）表示与对照组的显著性差异

Fig. 6 Growth curves of P. donghaiense in co-cultures with different content of U. prolifera dry powder

*(p<0.05) and **(p<0.01) indicate significant differences relative to the control

下载: 全尺寸图片幻灯片

图 7 不同含量的浒苔干粉末共培养对东海原甲藻生长抑制率（第8天）

Fig. 7 The growth inhibition rate of P. donghaiense in co-cultures with different contents of U. prolifera dry powder (the 8th day)

下载: 全尺寸图片幻灯片

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