黄、东海春季海水胞外酶活性水平分布特征研究

张正雨; 田继远; 于娟; 杨桂朋; 崔煜; 陈得萍; 高萍; 许瑞; 陈容

doi:10.3969/j.issn.0253-4193.2020.04.001

黄、东海春季海水胞外酶活性水平分布特征研究

doi: 10.3969/j.issn.0253-4193.2020.04.001

张正雨^1,,
田继远³,
于娟^{1, 2, ,},
杨桂朋^{1, 2},
崔煜¹,
陈得萍¹,
高萍⁴,
许瑞¹,
陈容¹

1.
中国海洋大学海洋化学理论与工程技术教育部重点实验室，山东青岛 266100
2.
青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室，山东青岛 266071
3.
青岛农业大学食品科学与工程学院，山东青岛 266109
4.
自然资源部第一海洋研究所，山东青岛 266061

基金项目: 国家重点研发计划(2016YFA0601302)；青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室渔业科技青年人才计划(2018-MFS-T16)；国家自然科学基金(41876122，41776085)；青岛海洋科学与技术试点国家实验室“鳌山人才”计划(2015ASTP)；国家自然科学基金创新研究群体项目(41521064)。

详细信息

作者简介:
张正雨(1995-)，女，山东省莱西市人，主要研究方向为海洋有机碳的降解。E-mail：1366738067@qq.com

通讯作者:
于娟，女，副教授，主要从事DMS的生物地球化学循环、海洋生态学研究。E-mail：yuetian@ouc.edu.cn

中图分类号: P714⁺.4
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- 被引次数: 0
出版历程
- 收稿日期: 2019-05-27
- 修回日期: 2019-09-16
- 网络出版日期: 2020-11-18
- 刊出日期: 2020-04-25

Horizontal distribution of extracellular enzyme activities in the Yellow Sea and the East China Sea in spring

Zhang Zhengyu^1
,,
Tian Jiyuan³,
Yu Juan^{1, 2
, ,},
Yang Guipeng^{1, 2},
Cui Yu¹,
Chen Deping¹,
Gao Ping⁴,
Xu Rui¹,
Chen Rong¹

1.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China
2.
Marine Ecology and Environmental Science Laboratory, Pilot National Laboratory for Marine science and Technology (Qingdao), Qingdao 266071, China
3.
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
4.
First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China

摘要

摘要: 海水胞外酶活性可以指示有机物的分布特征以及微生物的营养状况。我们测定了2017年3月25日至4月15日黄海和东海44个大面站以及2018年4月28日至29日胶州湾湾口附近海域10个站位表层海水中的8种胞外酶活性并研究了其分布特征。2017年春季黄、东海表层海水中碱性磷酸酶和脂肪酶活性较高，高值区出现在苏北沿岸和南黄海中部，碱性磷酸酶与磷酸盐浓度之间呈正相关。其余6种酶(肽酶、几丁质酶、纤维素酶、α-D-葡萄糖苷酶、β-D-半乳糖苷酶、木糖苷酶)活性高值区出现在长江口以东的外海，东海的β-D-半乳糖苷酶、木糖苷酶平均酶活性显著高于黄海。8种酶活性平均值排列顺序由大到小为：碱性磷酸酶、脂肪酶、肽酶、几丁质酶、α-D-葡萄糖苷酶、β-D-半乳糖苷酶、纤维素酶、木糖苷酶，其中α-D-葡萄糖苷酶和β-D-半乳糖苷酶的活性基本一致。2018年春季胶州湾附近海域海水中碱性磷酸酶、脂肪酶、木糖苷酶活性分布为近岸高于远岸，几丁质酶活性为近岸低于远岸。8种酶活性平均值排列顺序由大到小为：碱性磷酸酶、脂肪酶、肽酶、木糖苷酶、α-D-葡萄糖苷酶、β-D-半乳糖苷酶、几丁质酶、纤维素酶，其中几丁质酶和纤维素酶的活性基本一致。黄海的碱性磷酸酶和脂肪酶平均酶活性均显著高于东海和胶州湾附近海域。糖类水解酶(几丁质酶、纤维素酶、α-D-葡萄糖苷酶、β-D-半乳糖苷酶、木糖苷酶)平均酶活性在黄海最低。本文的结果对于理解中国近海海水有机碳的分布、浮游植物及异养细菌对有机碳的降解具有重要意义。
- 胞外酶活性 /
- 碱性磷酸酶 /
- 肽酶 /
- 脂肪酶
Abstract: The extracellular enzyme activity of seawater can indicate the distribution characteristics of organic and the nutritional status of microorganisms. Activities of eight extracellular enzymes, including alkaline phosphatase, lipase, leucine aminopeptidase, chitinase, cellulose, xylosidase, α-D-glucosidase and β-D-galactosidase, were measured in the surface seawaters collected from 44 stations of the Yellow Sea and the East China Sea from March 25 to April 15, 2017 and from 10 stations of the Jiaozhou Bay nearby waters from April 28 to 29, 2018. In the spring of 2017, the high-value areas of alkaline phosphatase and lipase appeared in the northern coast of Jiangsu and the middle of the Yellow Sea, alkaline phosphatase activity and phosphate concentration were positively correlated. High activity areas of the other six enzymes (leucine aminopeptidase, chitinase, cellulose, xylosidase, α-D-glucosidase and β-D-galactosidase) appeared in the open sea outside the east of the Changjiang River Estuary. The average activities of β-D-galactosidase and xylosidase were significantly higher in the East China Sea than in the Yellow Sea. The decreasing order of average enzyme activities of the eight enzymes was as follows: alkaline phosphatase, lipase, leucine aminopeptidase, chitinase, α-D-glucosidase, β-D-galactosidase, cellulose, xylosidase, the values of α-D-glucosidase and β-D-galactosidase are proximate. The activities of alkaline phosphates, lipase and xylosidase in the Jiaozhou Bay nearby waters in spring 2018 decreased from nearshore to far shore. The decreasing order of the average enzyme activity of the eight enzymes was as follows: alkaline phosphatase, lipase, leucine aminopeptidase, xylosidase, α-D-glucosidase, β-D-galactosidase, cellulose, chitinase, the values of cellulose and chitinase are proximate. The alkaline phosphatase and lipase activities in the Yellow Sea were significantly higher than those in the East China Sea and Jiaozhou Bay. The average enzymatic activities of polysaccharide hydrolases (chitinase, cellulose, α-D-glucosidase, β-D-galactosidase and xylosidase) were the lowest in the Yellow Sea compared with those in the East China Sea and Jiaozhou Bay nearby waters. Our results are beneficial to understand the degradation of organic carbon by plankton and heterotrophic bacteria and the distribution of marine organic carbon in the coastal water of China.
- extracellular enzyme activity /
- alkaline phosphatase /
- leucine aminopeptidase /
- lipase

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图 1 2017年春季黄、东海（a）和2018年春季胶州湾附近海域（b）采样站位

Fig. 1 Sampling stations in the Yellow Sea and the East China Sea in spring 2017 (a) and in the Jiaozhou Bay nearby waters in spring 2018 (b)

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图 2 2017年春季黄、东海表层海水温度、盐度及8种胞外酶活性水平分布

Fig. 2 The distributions of temperature, salinity and eight extracellular enzyme activities in the surface waters of the Yellow Sea and the East China Sea during spring 2017

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图 3 2018年春季胶州湾附近海域表层海水温度、盐度及8种胞外酶活性水平分布

Fig. 3 The distributions of temperature, salinity and eight extracellular enzyme activities in the surface waters of the Jiaozhou Bay nearby waters during spring 2018

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图 4 2017年春季黄、东海和2018年春季胶州湾附近海域8种胞外酶的平均酶活性

Fig. 4 Average activities of eight extracellular enzymes in the Yellow Sea and the East China Sea in spring 2017 and in Jiaozhou Bay nearby waters in spring 2018

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表 1 2017年春季黄、东海表层海水温度、盐度和胞外酶活性的变化范围及平均值

Tab. 1 Variation and average of temperatures, salinities and eight extracellular enzyme activities in the Yellow Sea and the East China Sea during spring 2017

	温度/℃	盐度	酶活性/pmol·L⁻¹·h⁻¹
	温度/℃	盐度	AP	LIP	AMP	CHI	AGLU	BGAL	CEL	XYL
变化范围	6.91～24.47	25.81～34.94	1 313～38 530	424～23 473	850～3 355	29～547	9～558	2～290	10～456	14～185
平均值	14.00	32.59	5 891±8 186	4 760±5 268	2 241±729	235±131	155±184	155±97	136±113	71±50
注：±表示标准偏差。

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表 2 2018年春季胶州湾附近海域表层海水温度、盐度和胞外酶活性的变化范围及平均值

Tab. 2 Variation and average of temperatures, salinities and eight extracellular enzyme activities in the Jiaozhou Bay nearby waters during spring 2018

	温度/℃	盐度	酶活性/pmol·L⁻¹·h⁻¹
	温度/℃	盐度	AP	LIP	AMP	XYL	AGLU	BGAL	CHI	CEL
变化范围	9.41～11.29	31.92～32.33	0～4 621	473～4 442	792～2 050	14～1 142	18～346	12～423	0～366	0～386
平均值	10.55	32.15	1 865±1 332	1 821±1 124	1 499±417	397±304	186±112	133±116	109±126	106±156
注：±表示标准偏差。

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表 3 2017年春季黄、东海和2018年春季胶州湾附近海域8种胞外酶活性之间的相关性分析

Tab. 3 Correlation analysis between eight extracellular enzyme activities of the Yellow Sea and the East China Sea during spring 2017 and the Jiaozhou Bay nearby waters during spring 2018

		AP	LIP	AMP	CHI	AGLU	BGAL	CEL	XYL
黄、东海	AP	1
	LIP	0.883**	1
	AMP	−0.110	−0.115	1
	CHI	−0.191	−0.045	0.001	1
	AGLU	0.292	0.199	0.165	0.165	1
	BGAL	−0.280	−0.250	0.112	0.606**	0.195	1
	CEL	0.314*	0.190	0.152	0.157	0.862**	0.180	1
	XYL	−0.161	−0.018	0.236	0.289	0.024	0.270	−0.033	1
胶州湾附近海域	AP	1
	LIP	0.369	1
	AMP	−0.441	0.313	1
	CHI	−0.306	−0.418	−0.207	1
	AGLU	−0.606	0.093	0.546	0.322	1
	BGAL	−0.122	−0.396	0.043	0.461	0.271	1
	CEL	0.116	0.611	0.394	−0.528	0.049	−0.344	1
	XYL	0.817**	0.314	−0.208	−0.429	−0.524	0.028	0.241	1
注：*表示p<0.01；表示p<0.05。

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表 4 温度、盐度及Chl a与8种胞外酶活性的相关性分析

Tab. 4 Relationship between temperature, salinity, Chl a and eight extracellular enzyme activities

胞外酶	黄、东海			胶州湾附近海域
胞外酶	温度	盐度	Chl a	温度	盐度
AP	−0.283	−0.069	−0.165	0.511	−0.488
LIP	−0.360*	−0.214	−0.135	0.146	−0.851**
AMP	−0.204	0.103	0.032	−0.561	−0.042
CHI	0.326*	−0.193	−0.018	0.063	0.500
AGLU	0.136	0.070	−0.038	−0.203	−0.014
BGAL	0.412**	0.011	−0.033	−0.315	0.376
CEL	0.159	0.072	−0.012	−0.476	−0.598
XYL	−0.291	−0.106	0.082	0.180	−0.477
注: *表示p<0.01；表示p<0.05。

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