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黄、东海春季海水胞外酶活性水平分布特征研究

张正雨 田继远 于娟 杨桂朋 崔煜 陈得萍 高萍 许瑞 陈容

张正雨,田继远,于娟,等. 黄、东海春季海水胞外酶活性水平分布特征研究[J]. 海洋学报,2020,42(4):1–11,doi:10.3969/j.issn.0253−4193.2020.04.001
引用本文: 张正雨,田继远,于娟,等. 黄、东海春季海水胞外酶活性水平分布特征研究[J]. 海洋学报,2020,42(4):1–11,doi:10.3969/j.issn. 0253−4193.2020.04.001
Zhang Zhengyu,Tian Jiyuan,Yu Juan, et al. Horizontal distribution of extracellular enzyme activities in the Yellow Sea and the East China Sea in spring[J]. Haiyang Xuebao,2020, 42(4):1–11,doi:10.3969/j.issn.0253−4193.2020.04.001
Citation: Zhang Zhengyu,Tian Jiyuan,Yu Juan, et al. Horizontal distribution of extracellular enzyme activities in the Yellow Sea and the East China Sea in spring[J]. Haiyang Xuebao,2020, 42(4):1–11,doi:10.3969/j.issn.0253−4193.2020.04.001

黄、东海春季海水胞外酶活性水平分布特征研究

doi: 10.3969/j.issn.0253-4193.2020.04.001
基金项目: 国家重点研发计划(2016YFA0601302);青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室渔业科技青年人才计划(2018-MFS-T16);国家自然科学基金(41876122,41776085);青岛海洋科学与技术试点国家实验室“鳌山人才”计划(2015ASTP);国家自然科学基金创新研究群体项目(41521064)。
详细信息
    作者简介:

    张正雨(1995-),女,山东省莱西市人,主要研究方向为海洋有机碳的降解。E-mail:1366738067@qq.com

    通讯作者:

    于娟,女,副教授,主要从事DMS的生物地球化学循环、海洋生态学研究。E-mail:yuetian@ouc.edu.cn

  • 中图分类号: P714+.4

Horizontal distribution of extracellular enzyme activities in the Yellow Sea and the East China Sea in spring

  • 摘要: 海水胞外酶活性可以指示有机物的分布特征以及微生物的营养状况。我们测定了2017年3月25日至4月15日黄海和东海44个大面站以及2018年4月28日至29日胶州湾湾口附近海域10个站位表层海水中的8种胞外酶活性并研究了其分布特征。2017年春季黄、东海表层海水中碱性磷酸酶和脂肪酶活性较高,高值区出现在苏北沿岸和南黄海中部,碱性磷酸酶与磷酸盐浓度之间呈正相关。其余6种酶(肽酶、几丁质酶、纤维素酶、α-D-葡萄糖苷酶、β-D-半乳糖苷酶、木糖苷酶)活性高值区出现在长江口以东的外海,东海的β-D-半乳糖苷酶、木糖苷酶平均酶活性显著高于黄海。8种酶活性平均值排列顺序由大到小为:碱性磷酸酶、脂肪酶、肽酶、几丁质酶、α-D-葡萄糖苷酶、β-D-半乳糖苷酶、纤维素酶、木糖苷酶,其中α-D-葡萄糖苷酶和β-D-半乳糖苷酶的活性基本一致。2018年春季胶州湾附近海域海水中碱性磷酸酶、脂肪酶、木糖苷酶活性分布为近岸高于远岸,几丁质酶活性为近岸低于远岸。8种酶活性平均值排列顺序由大到小为:碱性磷酸酶、脂肪酶、肽酶、木糖苷酶、α-D-葡萄糖苷酶、β-D-半乳糖苷酶、几丁质酶、纤维素酶,其中几丁质酶和纤维素酶的活性基本一致。黄海的碱性磷酸酶和脂肪酶平均酶活性均显著高于东海和胶州湾附近海域。糖类水解酶(几丁质酶、纤维素酶、α-D-葡萄糖苷酶、β-D-半乳糖苷酶、木糖苷酶)平均酶活性在黄海最低。本文的结果对于理解中国近海海水有机碳的分布、浮游植物及异养细菌对有机碳的降解具有重要意义。
  • 图  1  2017年春季黄、东海(a)和2018年春季胶州湾附近海域(b)采样站位

    Fig.  1  Sampling stations in the Yellow Sea and the East China Sea in spring 2017 (a) and in the Jiaozhou Bay nearby waters in spring 2018 (b)

    图  2  2017年春季黄、东海表层海水温度、盐度及8种胞外酶活性水平分布

    Fig.  2  The distributions of temperature, salinity and eight extracellular enzyme activities in the surface waters of the Yellow Sea and the East China Sea during spring 2017

    图  3  2018年春季胶州湾附近海域表层海水温度、盐度及8种胞外酶活性水平分布

    Fig.  3  The distributions of temperature, salinity and eight extracellular enzyme activities in the surface waters of the Jiaozhou Bay nearby waters during spring 2018

    图  4  2017年春季黄、东海和2018年春季胶州湾附近海域8种胞外酶的平均酶活性

    Fig.  4  Average activities of eight extracellular enzymes in the Yellow Sea and the East China Sea in spring 2017 and in Jiaozhou Bay nearby waters in spring 2018

    表  1  2017年春季黄、东海表层海水温度、盐度和胞外酶活性的变化范围及平均值

    Tab.  1  Variation and average of temperatures, salinities and eight extracellular enzyme activities in the Yellow Sea and the East China Sea during spring 2017

    温度/℃盐度酶活性/pmol·L−1·h−1
    APLIPAMPCHIAGLUBGALCELXYL
    变化范围6.91~24.4725.81~34.941 313~38 530424~23 473850~3 35529~5479~5582~29010~45614~185
    平均值14.0032.595 891±8 1864 760±5 2682 241±729235±131155±184155±97136±11371±50
      注:±表示标准偏差。
    下载: 导出CSV

    表  2  2018年春季胶州湾附近海域表层海水温度、盐度和胞外酶活性的变化范围及平均值

    Tab.  2  Variation and average of temperatures, salinities and eight extracellular enzyme activities in the Jiaozhou Bay nearby waters during spring 2018

    温度/℃盐度酶活性/pmol·L−1·h−1
    APLIPAMPXYLAGLUBGALCHICEL
    变化范围9.41~11.2931.92~32.330~4 621473~4 442792~2 05014~1 14218~34612~4230~3660~386
    平均值10.5532.151 865±1 3321 821±1 1241 499±417397±304186±112133±116109±126106±156
      注:±表示标准偏差。
    下载: 导出CSV

    表  3  2017年春季黄、东海和2018年春季胶州湾附近海域8种胞外酶活性之间的相关性分析

    Tab.  3  Correlation analysis between eight extracellular enzyme activities of the Yellow Sea and the East China Sea during spring 2017 and the Jiaozhou Bay nearby waters during spring 2018

    APLIPAMPCHIAGLUBGALCELXYL
    黄、东海AP1
    LIP0.883**1
    AMP−0.110−0.1151
    CHI−0.191−0.0450.0011
    AGLU0.2920.1990.1650.1651
    BGAL−0.280−0.2500.1120.606**0.1951
    CEL0.314*0.1900.1520.1570.862**0.1801
    XYL−0.161−0.0180.2360.2890.0240.270−0.0331
    胶州湾附近海域AP1
    LIP0.3691
    AMP−0.4410.3131
    CHI−0.306−0.418−0.2071
    AGLU−0.6060.0930.5460.3221
    BGAL−0.122−0.3960.0430.4610.2711
    CEL0.1160.6110.394−0.5280.049−0.3441
    XYL0.817**0.314−0.208−0.429−0.5240.0280.2411
      注:**表示p<0.01;*表示p<0.05。
    下载: 导出CSV

    表  4  温度、盐度及Chl a与8种胞外酶活性的相关性分析

    Tab.  4  Relationship between temperature, salinity, Chl a and eight extracellular enzyme activities

    胞外酶黄、东海胶州湾附近海域
    温度盐度Chl a温度盐度
    AP−0.283−0.069−0.1650.511−0.488
    LIP−0.360*−0.214−0.1350.146−0.851**
    AMP−0.2040.1030.032−0.561−0.042
    CHI0.326*−0.193−0.0180.0630.500
    AGLU0.1360.070−0.038−0.203−0.014
    BGAL0.412**0.011−0.033−0.3150.376
    CEL0.1590.072−0.012−0.476−0.598
    XYL−0.291−0.1060.0820.180−0.477
      注: **表示p<0.01;*表示p<0.05。
    下载: 导出CSV
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  • 收稿日期:  2019-05-27
  • 修回日期:  2019-09-16
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