Response of catalase in Antarctic ice alga Chlamydomonas sp. ICE-L to heat stress
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摘要: 为了研究南极冰藻的热胁迫应答机制，本文根据转录组测序得到的冰藻Chlamydomonas sp. ICE-L过氧化氢酶CiCAT基因分析了其编码蛋白的特征，同时研究了CiCAT基因表达和过氧化氢酶活性在培养温度升高时的响应变化情况。结果表明：CiCAT基因序列全长为2 066 bp，编码492个氨基酸。在过氧化氢酶的系统进化树中，南极冰藻与其他绿藻聚类为一个分支。CiCAT编码的蛋白序列与盐藻和红球藻的过氧化氢酶序列相似性较高，分别为80.5%和78.9%。当南极冰藻处于热胁迫条件下，CiCAT基因的相对表达量和过氧化氢酶活均呈现出先上升后下降的趋势，但在胁迫24 h时CiCAT基因的表达量变化不明显，而实验组的酶活显著高于对照组。在胁迫72 h时，基因表达量和酶活均达到最高值。研究初步表明，与在常温藻类和高等植物中的功能相似，在经受热胁迫的情况下，南极冰藻中的抗氧化酶系统也发挥着重要作用。Abstract: To investigate the heat stress responding strategies of Antarctic ice algae, the characteristics of a catalase gene CiCAT from the transcriptome of Antarctic ice alga Chlamydomonas sp. ICE-L were analyzed. The length of CiCAT is 2 066 bp encoding a catalase of 492 amino acids. In the phylogenetic tree of catalase amino acid sequences, Antarctic ice alga is clustered with green algae. The amino acid sequence identities of CiCAT are about 80.5% and 78.9% to the catalase from Dunaliella salina and Haematococcus lacustris, respectively. The changes of CiCAT gene expression and catalase activity in Antarctic ice alga were also investigated. Under heat stress, both the relative expression state of CiCAT gene and catalase activity changed from up-regulation to down-regulation over time. After heat stress treatment for 24 h, the expression of CiCAT gene was almost unchanged, while the enzyme activity in the heat treatment group was significantly higher than that in the control group. After 72 h's heat stress treatment, both gene expression and enzyme activity reached the highest level. Our preliminary results show that antioxidase system plays an important role in Antarctic ice algae responding to heat stress, which is similar to that in temperate algae and higher plants.
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Key words:
- Antarctic ice alga /
- heat stress /
- catalase
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图 1 CiCAT基因编码蛋白保守结构域分析（https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?RID=NTDBEUUU014&mode=all）
Fig. 1 Conserved domain of encoded protein by ice algae CiCAT gene (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?RID=NTDBEUUU014&mode=all)
图 2 冰藻与其他物种过氧化氢酶氨基酸序列的系统进化树分析
Fig. 2 Phylogenetic tree of catalase amino acid sequences between ice algae and other species
图 3 热胁迫下南极冰藻的生长曲线
OD750表示波长750 nm处的吸光度
Fig. 3 Growth curves of Antarctic ice alga under heat stress
OD750 is optical density at wavelength 750 nm
图 4 热胁迫下南极冰藻CiCAT基因随时间的相对表达变化
Fig. 4 The expression of CiCAT gene of Antarctic ice alga under heat stress
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