New record and toxic species of the genus Pseudo-nitzschia from southeastern China waters
-
摘要: 拟菱形藻属(Pseudo-nitzschia)物种是一类备受关注的海洋有毒有害硅藻,我国相关研究仍然不足,已报道的物种多样性偏低,产毒物种也较少。为了解我国海域拟菱形藻属的物种多样性,阐述产毒物种信息,在我国东南海域建立了8个拟菱形藻单克隆培养株系,结合形态学和分子系统学数据,鉴定到2个物种:环孔拟菱形藻(Ps.circumpora C.H.Lim,C.P.Leaw&P.T.Lim)和多列拟菱形藻[Ps.multiseries(Hasle) Hasle],其中环孔拟菱形藻是我国的新记录种。利用高效液相色谱-质谱联用法检测了藻株的多莫酸(domoic acid,DA)特征,在5个多列拟菱形藻株系中检测到DA,单细胞产毒水平为1.01~96.11 fg。此外,利用卤虫(Artemia salina L.)和强额拟哲水蚤(Paracalanus crassirostris Dahl)对多列拟菱形藻的产毒株系(MC4177)进行诱导,发现能够提高其单细胞产毒水平,提高幅度在26~55倍。Abstract: The diatom genus Pseudo-nitzschia Peragallo is a kind of harmful bloom microalgae. But the relative studies are still insufficient in China, and low species diversity and few toxic species have been recorded. To explore the diversity and verify the toxicity, 8 monoclonal strains were established from southeastern China waters. Morphology was observed under light and transmission electron microscopy, and the sequences of ribosomal transcribed spacer region were analyzed to build phylogenetic relationships. Combined morphology and molecular data, two Pseudo-nitzschia taxa were identified, i.e., Ps. circumpora C. H. Lim, C. P. Leaw & P. T. Lim and Ps. multiseries (Hasle) Hasle, with the former as a new record for China. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), domoic acid (DA) was detected from 5 Ps. multiseries strains, with DA concentrations around 1.01-96.11 fg/cell. Meanwhile, a toxic Ps. multiseries strain (MC4177) was selected and induced by mix-culture with Artemia salina L. and Paracalanus crassirostris Dahl, respectively. The result showed that DA concentration increased greatly, about 26-55 times after induction.
-
Lelong A, Hégaret H, Soudant P, et al. Pseudo-nitzschia (Bacillariophyceae) species, domoic acid and amnesic shellfish poisoning:revisiting previous paradigms[J]. Phycologia, 2012, 51(2):168-216. Trainer V L, Bates S S, Lundholm N, et al. Pseudo-nitzschia physiological ecology, phylogeny, toxicity, monitoring and impacts on ecosystem health[J]. Harmful Algae, 2012, 14:271-300. Bates S S, Bird C J, de Freitas A S W, et al. Pennate diatom Nitzschia pungens as the primary source of domoic acid, a toxin in shellfish from eastern Prince Edward Island, Canada[J]. Canadian Journal of Fisheries and Aquatic Sciences, 1989, 46(7):1203-1215. Bates S S, Garrison D L, Horner R A. Bloom dynamics and physiology of domoic-acid-producing Pseudo-nitzschia species[M]//Anderson D M, Cembella A D, Hallegraeff G M. Physiological Ecology of Harmful Algal Blooms. Heidelberg:Springer, 1998:267-292. Lundholm N, Moestrup Ø, Akselmann R, et al. IOC-UNESCO taxonomic reference list of harmful micro algae[EB/OL]. http://www.marinespecies.org/hab. Li Yang, Huang Chunxiu, Xu Guoshuang, et al. Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters[J]. Harmful Algae, 2017, 67:119-130. 黄春秀, 董焕嫦, 吴海燕, 等. 福氏拟菱形藻(Pseudo-nitzschia fukuyoi)——中国产毒拟菱形藻的新记录[J]. 海洋与湖沼, 2017, 48(5):1014-1021. Huang Chunxiu, Dong Huanchang, Wu Haiyan, et al. Pseudo-nitzschia fukuyoi-a new record of toxic Pseudo-nitzschia taxa from China[J]. Oceanologia et Limnologia Sinica, 2017, 48(5):1014-1021. 陈西平, 王成斌, 胡俊明, 等. HPLC方法检测水及水生动物中软骨藻酸[J]. 卫生研究, 2001, 30(4):247-248. Chen Xiping, Wang Chengbin, Hu Junming, et al. Determination of domoic acid in water and aquatic animals by high performance liquid chromatography[J]. Journal of Hygiene Research, 2001, 30(4):247-248. 李大志, 祝文君, 宋文斌, 等. 记忆缺失性贝类毒素的主要成分——软骨藻酸的毛细管电泳分析[J]. 色谱, 2002, 20(2):125-128. Li Dazhi, Zhu Wenjun, Song Wenbin, et al. Capillary electrophoretic analysis of amnesic shellfish toxin-domoic acid[J]. Chinese Journal of Chromatography, 2002, 20(2):125-128. 王恒. 舟山海域贝类海产品中软骨藻酸含量调查[J]. 中国卫生检验杂志, 2011, 21(12):2986-2988, 2992. Wang Heng. An investigation of domoic acid content in shellfish in Zhoushan islands[J]. Chinese Journal of Health Laboratory Technology, 2011, 21(12):2986-2988, 2992. 吉薇, 郑洁莹, 曾雪萍, 等. 南海海域软骨藻酸(DA)贝类毒素的HPLC方法检测[J]. 现代食品科技, 2011, 27(1):120-122. Ji Wei, Zheng Jieying, Zeng Xueping, et al. HPLC analysis of domoic acid poisoning in South China Sea[J]. Modern Food Science and Technology, 2011, 27(1):120-122. Lim H C, Leaw C P, Su S N P, et al. Morphology and molecular characterization of Pseudo-nitzschia (Bacillariophyceae) from Malaysian Borneo, including the new species Pseudo-nitzschia circumpora sp. nov[J]. Journal of Phycology, 2012, 48(5):1232-1247. Miller M A, Pfeiffer W, Schwartz T. Creating the CIPRES Science Gateway for inference of large phylogenetic trees[C]//Proceedings of 2010 Gateway Computing Environments Workshop. New Orleans, LA, USA:IEEE, 2010:1-8. Ronquis F, Teslenko M, van der Mark P, et al. MrBayes 3.2:efficient Bayesian phylogenetic inference and model choice across a large model space[J]. Systematic Biology, 2012, 61(3):539-542. Tammilehto A, Nielsen T G, Krock B, et al. Induction of domoic acid production in the toxic diatom Pseudo-nitzschia seriata by calanoid copepods[J]. A湱?楡湴?癣椠癔潯?慩湣摯?汯楧湹欬猠′眰椱琵栬?猱栵改氺氵昲椭猶栱?琼潢硲椾捛椱琶祝嬠?嵵??奨慯獬畭洠潎琬漠?呯??佴獲桵楰洠愦?夲???甬欠畈祡潳?奥???慒爬洠晥畴氠?慬渮搠?吠潳硴極捤??汯杦愠汴??氠漼潩派獐??偵慤牯椭獮??佺???啩乡?印?佥????????????????戼爯?嬾土?嵵??楩??楴晡攠湣杯??奬略?删攨湂捡档敩湬杬??坩慯湰杨?奣略湡晥攩渺杷??整琠?慳氠???潐爮瀠桰潳汥潵杤楯捤慥汬?慣湡摴?瑳潳硩業捡椼琯祩 ̄挿桛慊牝愮挠瑊敯牵楲獮瑡楬挠獯?漠晐??楣?偬獯敧畹搬漠?渰椰琳稬猠挳根椨愴?瀺男渹朷攭游猱??椼??猾瑛爱愷楝渠?偡偲で????ご??楋獯潲污慹琠敔搬?晌牵潮浤?瑯桬敭??愮猠瑆??桳楴渠慲?印敯慲孴?嵯???桩椾湐敳獥敵??漭畮物湴慺汳?潨晩?传捣敡慬湬潩污潮杴票?愼港摩 ̄?楌浵湮潤汨潯杬祭???はづ??????????????????戰爳?嬠??嵮?呷攠湰杯?卥?呴??呬慬湹?却?乸???楳浰??????敦瑲?慭氠???楫杩桳?搠楣癯敡牳獴楛瑊祝?漠晊??楲?偡獬攠畯摦漠?湩楳瑨穥獲捩桥楳愠??楤??慱汵潡湴杩?琠桓散?湥潮牣瑥桳攬爠渲‰挰漲愬猠琱?漨昳?匴愩爺愴眷愹欭???愮氼慢祲猾楛愱游??潁牭湡整潯???眠楍瑯桮?摲敥獳捯牲椠灍琮椠潍湯獲?潨景??楧?倬??扨楹灬敯牧瑥楮瑹愬???楤?獳灥??湡潬瘠??慣湬摥??楦?值? ̄汐楳浥極楤??楮??獺灳??湩潡瘠????慩捩椼氯汩愾爠楳潰瀮栠祮捯敶愮攠?孂?嵣???潡畲物湯慰汨?潣晥?灥栩示捡漠汰潳来祵??木っ??????????????????hin the P. pseudodelicatissima complex[J]. Phycologia, 2008, 47(5):487-497. Hasle G R. Pseudo-nitzschia pungens and P. multiseries (Bacillariophyceae):nomenclatural history, morphology, and distribution[J]. Journal of Phycology, 1995, 31(3):428-435. 董焕嫦, 黄春秀, 徐国双, 等. 中国沿海尖刺拟菱形藻的种下分类学研究[J]. 热带海洋学报, 2018, 37(1):12-19. Dong Huanchang, Huang Chunxiu, Xu Guoshuang, et al. Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters[J]. Journal of Tropical Oceanography, 2018, 37(1):12-19. Lundholm N, Moestrup Ø. The marine diatom Pseudo-nitzschia galaxiae sp. nov. (Bacillariophyceae):morphology and phylogenetic relationships[J]. Phycologia, 2002, 41(6):594-605. 徐国双, 李扬. 我国沿海拟菱形藻属的2新记录种及其产毒特征分析[J]. 热带亚热带植物学报, 2015, 23(6):614-624. Xu Guoshuang, Li Yang. Two new records of diatom genus Pseudo-nitzschia from Chinese waters and analysis of their domoic acid production[J]. Journal of Tropical and Subtropical Botany, 2015, 23(6):614-624. Cerino F, Orsini L, Sarno D, et al. The alternation of different morphotypes in the seasonal cycle of the toxic diatom Pseudo-nitzschia galaxiae[J]. Harmful Algae, 2005, 4(1):33-48. Rhodes L, White D, Syhre M. Pseudo-nitzschia species isolated from New Zealand coastal waters:domoic acid productio
点击查看大图
计量
- 文章访问数: 471
- HTML全文浏览量: 18
- PDF下载量: 162
- 被引次数: 0