饲料中添加壳寡糖对花鲈(<i>Lateolabrax japonicus</i>)幼鱼的生长、消化和血清生化指标的影响

胡晓伟; 上官静波; 黎中宝; 杨敏; 徐安乐

doi:10.3969/j.issn.0253-4193.2018.02.007

饲料中添加壳寡糖对花鲈(Lateolabrax japonicus)幼鱼的生长、消化和血清生化指标的影响

doi: 10.3969/j.issn.0253-4193.2018.02.007

集美大学水产学院, 福建厦门 361021;福建省海洋渔业资源与生态环境重点实验室, 福建厦门 361021

基金项目: 福建省科技重点项目（2015N0010）；厦门市科技项目（3502Z20143017）。

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- 收稿日期: 2017-07-15

Effects of dietary Chitosan oligosaccharide on the performance, digestion and serum biochemical indexes of the juvenile Japanese seabass (Lateolabrax japonicus)

Fisheries College, Jimei University, Xiamen 361021, China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen 361021, China

摘要

摘要: 本试验旨在研究壳寡糖对花鲈（Lateolabrax japonicus）幼鱼生长，肠道消化，血清生化指标及肠道菌群的影响。本试验选用360尾规格一致的花鲈幼鱼（19.37 g左右）为研究对象。壳寡糖饲料添加浓度设为6组：0%、0.2%、0.4%、0.6%、0.8%、1.0%。设3个平行组，养殖周期45 d。结果显示：（1）生长性能：添加0.6%和0.8%两组的增重率、特定生长率与对照组相比有提高（P>0.05）；（2）肠道消化酶：添加0.6%~1.0%的壳寡糖显著（P<0.05）提高胰蛋白酶活性，0.4%~0.8%的壳寡糖显著（P<0.05）提高了脂肪酶活性；（3）肠道菌群：0.6%~1.0%的壳寡糖显著降低了沙门氏菌数量（P<0.05），大肠杆菌的数量在0.8%时显著降低（P<0.05），双歧杆菌的数量在0.2%~0.6%时显著增加（P<0.05）；（4）血清生化指标：壳寡糖显著提高血清总蛋白和高密度脂蛋白胆固醇含量（P<0.05）；（5）血清非特异性免疫：添加0.6%的壳寡糖显著降低丙二醛（P>0.05），0.2%的壳寡糖增强超氧化物歧化酶、碱性磷酸酶和过氧化物酶活性，添加壳寡糖使溶菌酶的活性显著增强（P<0.05）。研究结果表明：饲料中添加0.6%~0.8%的壳寡糖对花鲈幼鱼生长性能、血脂及肠道健康影响效果最佳。
- 花鲈 /
- 壳寡糖 /
- 生长性能 /
- 非特异性免疫 /
- 肠道菌群
Abstract: This research was conducted to evaluate the effects of dietary supplementation with Chitosan-oligosaccharide (COS) on the growth performance, digestion, serum biochemical indexes and intestinal flora. A total of 360 juvenile Japanese seabass were randomly assigned to six treatment diets that included COS at concentrations of 0%,0.2%,0.4%,0.6%,0.8%,1.0% for 45 d. Each treatment had 3 replicates with 20. The results showed:(1) The growth performance:The weight gain and specific growth rate of Diet3(0.6%) and Diet4(0.8%) were increased compared with the control group (P>0.05); (2) The digestive ferment:The intestinal trypsin activity were significantly increased (P<0.05) in the Diet3(0.6%), Diet4(0.8%) and Diet5(1.0%). Intestinal lipase activity was remarkably increased at Diet2(0.4%),Diet3(0.6%) and Diet4(0.8%) (P<0.05). There was no significant difference in intestinal amylase activity (P>0.05). (3) The serum biochemical indexes:Compared with the control group, the contents of the serum total protein and high-density lipoprotein cholesterol (HDL-C) was obviously increased (P<0.05); (4) The nonspecific immunity:The contents of MDA was decreased significantly (P>0.05) on the Diet3(0.6%). The activities of superoxide dismutase, alkaline phosphatase and peroxidase were all increased (P>0.05) on the Diet1 (0.2%), and the serum lysozyme was significantly increased (P<0.05). (5) Intestinal flora:The number of Salmonella in Diet3 (0.6%), Diet 4(0.8%) and Diet5(1%), and E. coli was significantly decreased (P<0.05) in Diet(0.8%). The quantity of bifidobacteria increased significantly (P<0.05) in Diet1(0.2%), Diet2(0.4%) and Diet3(0.6%). In conclusion, these results indicate that COS can enhance growth and immunity of juvenile Japanese seabass,and the optimum addition level was 0.6%~0.8% in diet.
- Lateolabrax japonicas /
- chitosan-oligosaccharide /
- growth performance /
- non-specific immune /
- intestinal flora

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