Effects of Cu2+ on structure and peroxidase activity of Tegillarca granosa hemoglobin (Tg-HbⅡ)
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摘要: 本文利用Cu2+处理泥蚶血红蛋白(Tg-HbⅡ),分别检测Cu2+对Tg-HbⅡ的过氧化物酶活性及其结构的影响,同时检测Pro对Cu2+作用下Tg-HbⅡ的保护作用。结果表明:随着Cu2+浓度的增加,Tg-HbⅡ的过氧化物酶活性逐渐丧失,Cu2+抑制Tg-HbⅡ的过氧化物酶活性类型属于非竞争-竞争混合型抑制,其失活速率具有浓度依赖性,失活过程呈单相变化,表现为一级反应动力学,并且不同浓度Cu2+能影响Tg-HbⅡ的三级结构变化;另外,Pro对Tg-HbⅡ的空间结构及其过氧化物酶活性具有保护作用,能阻止Tg-HbⅡ的聚沉、恢复Tg-HbⅡ的结构与过氧化物酶活性。Abstract: We studied the effects of Cu2+ on the peroxidase activity and structure ofthe Tegillarca granosa hemoglobin (Tg-HbⅡ),and the protective action of Pro to the Tg-HbⅡthat has been treated by Cu2+. The results showed that the peroxidase activity of Tg-HbⅡ was gradually decreased with the increase of Cu2+ concentration, and the inhibition of Cu2+ to Tg-HbⅡperoxidase activity belonged to noncompetitive-competitive mixed inhibition type.The inactivation rate was in a concentration-dependent manner, and the inactivation process showed a single-phase change, which showed a first-order reaction kinetics. The results also indicated that different concentrations of Cu2+ could affect the tertiary structure of Tg-HbⅡ. In addition, Pro could protect the spatial structure of Tg-HbⅡ and restore its peroxidase activity destroyed by Cu2+, which successfully blocked Tg-HbⅡaggregation, recoverying the conformation and activity of Tg-HbⅡ.
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Key words:
- Cu2+ /
- Tegillarca granosa /
- hemoglobin /
- peroxidase /
- structure
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