Cu<sup>2+</sup>对泥蚶血红蛋白(Tg-HbⅡ)的过氧化物酶活性与结构的影响

张顺琴; 王素芳; 陈梦玫; 林志华

doi:10.3969/j.issn.0253-4193.2018.01.012

Cu²⁺对泥蚶血红蛋白(Tg-HbⅡ)的过氧化物酶活性与结构的影响

doi: 10.3969/j.issn.0253-4193.2018.01.012

浙江万里学院生物与环境学院水产种质资源高效利用技术省重点实验室, 浙江宁波 315100

基金项目: 浙江省基金项目（LQ15C190001）；浙江省重中之重生物工程学科资助（CX2016009）。

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- 收稿日期: 2017-06-26

Effects of Cu²⁺ on structure and peroxidase activity of Tegillarca granosa hemoglobin (Tg-HbⅡ)

Biology and Environment Key Laboratory of Aquatic Germplasm Resource, Zhejiang Wanli University, Ningbo 315100, China

摘要

摘要: 本文利用Cu²⁺处理泥蚶血红蛋白（Tg-HbⅡ），分别检测Cu²⁺对Tg-HbⅡ的过氧化物酶活性及其结构的影响，同时检测Pro对Cu²⁺作用下Tg-HbⅡ的保护作用。结果表明：随着Cu²⁺浓度的增加，Tg-HbⅡ的过氧化物酶活性逐渐丧失，Cu²⁺抑制Tg-HbⅡ的过氧化物酶活性类型属于非竞争-竞争混合型抑制，其失活速率具有浓度依赖性，失活过程呈单相变化，表现为一级反应动力学，并且不同浓度Cu²⁺能影响Tg-HbⅡ的三级结构变化；另外，Pro对Tg-HbⅡ的空间结构及其过氧化物酶活性具有保护作用，能阻止Tg-HbⅡ的聚沉、恢复Tg-HbⅡ的结构与过氧化物酶活性。
- Cu²⁺ /
- 泥蚶 /
- 血红蛋白 /
- 过氧化物酶 /
- 结构
Abstract: We studied the effects of Cu²⁺ on the peroxidase activity and structure ofthe Tegillarca granosa hemoglobin (Tg-HbⅡ),and the protective action of Pro to the Tg-HbⅡthat has been treated by Cu²⁺. The results showed that the peroxidase activity of Tg-HbⅡ was gradually decreased with the increase of Cu²⁺ concentration, and the inhibition of Cu²⁺ to Tg-HbⅡperoxidase activity belonged to noncompetitive-competitive mixed inhibition type.The inactivation rate was in a concentration-dependent manner, and the inactivation process showed a single-phase change, which showed a first-order reaction kinetics. The results also indicated that different concentrations of Cu²⁺ could affect the tertiary structure of Tg-HbⅡ. In addition, Pro could protect the spatial structure of Tg-HbⅡ and restore its peroxidase activity destroyed by Cu²⁺, which successfully blocked Tg-HbⅡaggregation, recoverying the conformation and activity of Tg-HbⅡ.
- Cu²⁺ /
- Tegillarca granosa /
- hemoglobin /
- peroxidase /
- structure

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