龙须菜配子体发育和不同品系四分孢子体四分孢子形成过程显微结构观察

米萍; 隋正红; 阙州; 周伟; 商二磊

doi:10.3969/j.issn.0253-4193.2017.08.010

龙须菜配子体发育和不同品系四分孢子体四分孢子形成过程显微结构观察

doi: 10.3969/j.issn.0253-4193.2017.08.010

中国海洋大学海洋生物遗传与育种教育部重点实验室, 山东青岛 266003

基金项目: 国家自然科学基金（31372529）；鳌山科技创新计划课题（2015ASKJ02）；中国博士后科学基金项目（2016M590662）。

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出版历程
- 收稿日期: 2016-10-28
- 修回日期: 2017-02-24

Microscopic observation of the reproductive development of gametophyte and tetrasporohyte of Gracilariopsis lemaneiformis (Rhodophyta)

The Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Ministry of Education, Qingdao 266003, China

摘要

摘要: 龙须菜配子体和四分孢子体在生殖结构的形成和发育的过程中，出现一系列的细胞形态和结构层面的变化。本实验以龙须菜雌雄配子体和不同品系龙须菜四分孢子体为实验材料，采用连续徒手切片的方法，观察龙须菜配子体发育和不同品系四分孢子体四分孢子形成过程。观察到，雌雄配子体发育经过幼苗期、毛细胞期、性成熟期，幼苗期和毛细胞期雌雄配子体间无明显差异，进入性成熟期后，产生生殖结构时才能将其区分开。四分孢子起源于四分孢子体表皮层细胞，初始为鲜红色直径约5~10 μm的细胞，逐渐生长形成成熟的直径约20~25 μm的四分孢子囊，并分裂为4个四分孢子聚集在四分孢子囊中，在藻体外，四分孢子释放变形成球形，龙须菜四分孢子的形成和发育过程伴随着髓部细胞中红藻淀粉颗粒的减少。其中"981"龙须菜形成较多的畸形四分孢子，可能是其表现低育的一个原因。龙须菜自基部到尖端的表皮层细胞层数、髓部细胞数目和体积均发生连续的变化。细胞连接广泛存在于龙须菜表皮层、皮层和髓部细胞之间，但各层细胞连接大小、数目、长度等都存在差异。释放的四分孢子可能会附着在四分孢子体上，固着器覆盖包绕四分孢子体甚至侵入到破损有伤口的四分孢子体内，形成四分孢子体和配子体在同一株上的世代混杂现象，这也可能是导致龙须菜表现遗传复杂性的原因。
- 龙须菜 /
- 配子体 /
- 四分孢子体 /
- 生殖结构 /
- 显微观察
Abstract: A series of cell morphology and structure changes were displayed during the development of reproductive structure of gametophyte and tetrasporaphyte of Gracilariopsis lemaneiformis. In this study, the gametophyte and different strains of tetrasporophyte were used to make continuous free hand section, in order to observe the development of the gametophyte and the formation of tetraspores in different strains of G. lemaneiformis. The result showed that the development of male and female gametophytes undertook seedling phage (SP), hair cell phage (HCP) and sexual mature phage (SMP). There was no significant difference between male and female gametophyte in SP and HCP. The male and female gametophyte were differentiable after entering SMP when the reproductive apparatus formed. The tetraspores were derived from the epidermal cells of tetrasprophyte, the initial tetrasporogonium was scarlet, and 5-10 μm in diameter. It later grew to 20-25 μm in diameter and became mature. The mature tetrasporogonium divided into four initial tetraspores gathered together, and then the tetraspores were released out of the matrix and became globular. During the development of tetraspores, the density of floridean starch granules decreased in medullary part. Cultivar 981 formed more deformed tetraspores, which may be one of the reasons leading to the low fertility of cultivar 981. The layers of epidermal cells, the numbers and the volume of medullary cells changed continuously from the basal to the tip part. Pit connections existed wildly in the epidermal, cortical and medullary cells, however varying by size, numbers and length between different layers. The released tetraspores were observed attaching on the tetrasporophyte, and the holdfast grew to cover the tetrasporophyte or invaded into the wound tissue of tetrasporophyte, which may cause gametophyte and tetrasporophyte generation promiscuous and lead to the genetic complexity of G. lemaneiformis.
- Gracilariopsis lemaneiformis /
- gametophyte /
- tetrasporophyte /
- reproductive apparatus /
- microscopic observation

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参考文献(40)

Lapointe B E, Ryther J H. Some aspects of the growth and yield of Gracilaria tikvahiae in culture[J]. Aquaculture, 1978, 15(3):185-193.

McHugh D J. Worldwide distribution of commercial resources of seaweeds including Gelidium[J]. Hydrobiologia, 1991, 221(1):19-29.

Chopin T, Buschmann A H, Halling C, et al. Integrating seaweeds into marine aquaculture systems:a key toward sustainability[J]. Journal of Phycology, 2001, 37(6):975-986.

Lu Huiming, Xie Haihui, Gong Yingxue, et al. Secondary metabolites from the seaweed Gracilaria lemaneiformis and their allelopathic effects on Skeletonema costatum[J]. Biochemical Systematics and Ecology, 2011, 39(4/6):397-400.

余杰, 王欣, 陈美珍, 等. 潮汕沿海龙须菜的营养成分和多糖组成分析[J]. 食品科学, 2006, 27(1):93-97. Yu Jie, Wang Xin, Chen Meizhen, et al. Analysis on nutritional components and polysaccharide composition of Gracilaria lemaneiformis from Chaoshan coast[J]. Food Science, 2006, 27(1):93-97.

Lu Huiming, Liao Xiaojian, Yang Yufeng, et al. Effects of extracts from Gracilaria lemaneiform on microalgae[J]. Ecological Science, 2008, 27(5):424-426.

徐永健, 钱鲁闽, 焦念志. 江蓠作为富营养化指示生物及修复生物的氮营养特性[J]. 中国水产科学, 2004, 11(3):276-280. Xu Yongjian, Qian Lumin, Jiao Nianzhi. Nitrogen nutritional character of Gracilaria as bioindicators and restoral plants of eutrophication[J]. Journal of Fishery Sciences of China, 2004, 11(3):276-280.

张学成, 费修绠, 王广策, 等. 江蓠属海藻龙须菜的基础研究与大规模栽培[J]. 中国海洋大学学报:自然科学版, 2009, 39(5):947-954. Zhang Xuecheng, Fei Xiugeng, Wang Guangce, et al. Genetic studies and large scale cultivation of Gracilaria lemaneiformis[J]. Periodical of Ocean University of China, 2009, 39(5):947-954.

孟琳, 徐涤, 陈伟洲, 等. 龙须菜新品系07-2的筛选及性状分析[J]. 中国海洋大学学报, 2009, 39(S1):94-98. Meng Lin, Xu Di, Chen Weizhou, et al. Selection and characterization of a new strain of Gracilaria lemaneiformis[J]. Periodical of Ocean University of China, 2009, 39(S1):94-98.

周伟, 隋正红. 龙须菜"鲁龙1号"[J]. 中国水产, 2016(3):56-57. Zhou Wei, Sui Zhenhong. The Cultivar "Lulong NO.1" of Gracilariopsis lemaneiformis (Gracilariaceae, Rhodophyta)[J]. China Fisheries, 2016(3):56-57.

张学成, 秦松, 马家海, 等. 海藻遗传学[M]. 北京:中国农业出版社, 2005. Zhang Xuecheng, Qin Song, Ma Jiahai, et al. The Genetic of Marine Algae[M]. Beijing:China Agriculture Press, 2005.

Dawson E Y. Studies of northeast pacific gracilariaceae[J]. Allan Hancock Foundation Publications, Occasional Papers, 1949, 7:1-105.

Fredericq S, Hommersand M H. Comparative morphology and taxonomic status of Gracilariopsis (Gracilariales, Rhodophyta)[J]. Journal of Phycology, 1989, 25(2):228-241.

Xu Di, Zhu Jianyi, He Yang, et al. Structure of reproductive apparatus of Gracilaria/Gracilariopsis lemaneiformis (Gracilariaceae, Rhodophyta)[J]. Science in China Series C:Life Sciences, 2008, 51(12):1137-1143.

Wang Jinguo, Sui Zhenghong, Hu Yiyi, et al. Assessment of photosynthetic performance, carboxylase activities, and ATP content during tetrasporic development in Gracilariopsis lemaneiformis (Gracilariaceae, Rhodophyta)[J]. Journal of Applied Phycology, 2016, 28(5):2939-2952.

Ye Naihai, Wang Hongxia, Wang Guangce. Formation and early development of tetraspores of Gracilaria lemaneiformis (Gracilaria, Gracilariaceae) under laboratory conditions[J]. Aquaculture, 2006, 254(1/4):219-226.

Zhou Wei, Ding Hongye, Sui Zhenghong, et al. Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique[J]. Chinese Journal of Oceanology and Limnology, 2014, 32(3):522-526.

Rover T, Simioni C, Hable W, et al. Ultrastructural and structural characterization of zygotes and embryos during development in Sargassum cymosum (Phaeophyceae, Fucales)[J]. Protoplasma, 2015, 252(2):505-518.

Dring D M. A periodic Acid-Schiff technique for staining fungi in higher plants[J]. New Phytologist, 1955, 54(2):277-279.

Huan Li, He Linwen, Zhang Baoyu, et al. AFLP and SCAR markers associated with the sex in Gracilaria lemaneiformis (Rhodophyta)[J]. Journal of Phycology, 2013, 49(4):728-732.

Kraft G T, Woelkerling W J. Rhodophyta[M]//Clayton M N, King R J. Biology of Marine Plants. Melbourne:Longman Cheshire, 1990:41-85.

Sjöstedt L G. Floridean Studies[M]. Otto Harrassowitz:CWK Gleerup, 1926.

Yamamoto H. Systematic and anatomical study of the genus Gracilaria in Japan[J]. Memoirs of the Faculty of Fisheries, Hokkaido University, 1978, 25(2):97-152.

Dixon P S. Biology of the Rhodophyta[M]. Edinburgh:Olivier and Bold, 1973:285.

Rueness J, Mathisen H A, Tananger T. Culture and field observations on Gracilaria verrucosa (Huds.) Papenf. (Rhodophyta) from Norway[J]. Botanica Marina, 1987, 30(3):267-276.

O'connor K A, West J A. The effect of light and nutrient conditions on hair formation in Spyridia filamentosa (Wulfen) Harvey (Rhodophyta)[J]. Botanica Marina, 1991, 34(4):359-364.

dos Santos R W, SchmidtÉ C, Bouzon Z L. Changes in ultrastructure and cytochemistry of the agarophyte Gracilaria domingensis (Rhodophyta, Gracilariales) treated with cadmium[J]. Protoplasma, 2013, 250(1):297-305.

Yu Jiang, Yang Yufeng. Physiological and biochemical response of seaweed Gracilaria lemaneiformis to concentration changes of N and P[J]. Journal of Experimental Marine Biology and Ecology, 2008, 367(2):142-148.

Lee R E. The pit connections of some lower red algae:ultrastructure and phylogenetic significance[J]. British Phycological Journal, 1971, 6(1):29-38.

Ramus J. Pit connection formation in the red alga Pseudogloiophloea[J]. Journal of Phycology, 1969, 5(1):57-63.

Wetherbee R, Quirk H M. The fine structure of secondary pit connection formation between the red algal alloparasite Holmsella australis and its red algal host Gracilaria furcellata[J]. Protoplasma, 1982, 110(3):166-176.

Bourne V L, Conway E, Cole K. On the ultrastructure of pit connections in the conchocelis phase of the red alga Porphyra perforata J. Ag[J]. Phycologia, 1970, 9(1):79-81.

王素娟, 裴鲁青, 段德麟. 中国常见红藻超微结构[M]. 宁波:宁波出版社, 2004:138-158. Wang Sujuan, Pei Luqing, Duan Delin. Ultrastructure of Common Red Seaweeds in China[M]. Ningbo:Ningbo Press, 2004:138-158.

许璞, 张学成, 王素娟, 等. 中国主要经济海藻的繁殖与发育[M]. 北京:中国农业出版社, 2013:85-90. Xu Pu, Zhang Xucheng, Wang Sujuan, et al. Reproduction and Development of Major Economic Marine Algae in China[M]. Beijing:China Agriculture Press, 2013:85-90.

Jones W E. Effect of spore coalescence on the early development of Gracilaria verrucosa (Hudson) papenfuss[J]. Nature, 1956, 178(4530):426-427.

Muñoz A A, Santelices B. Quantification of the effects of sporeling coalescence on the early development of Gracilaria chilensis (Rhodophyta)[J]. Journal of Phycology, 1994, 30(3):387-392.

Santelices B, Correa J A, Aedo D, et al. Convergent biological processes in coalescing Rhodophyta[J]. Journal of Phycology, 1999, 35(6):1127-1149.

Santelices B, Aedo D, Varela D. Causes and implications of intra-clonal variation in Gracilaria chilensis (Rhodophyta)[J]. Journal of Applied Phycology, 1995, 7(3):283-290.

Santelices B, Correa J A, Meneses I, et al. Sporeling coalescence and intraclonal variation in Gracilaria chilensis (Gracilariales, Rhodophyta)[J]. Journal of Phycology, 1996, 32(2):313-322.

Kain J M, Destombe C. A review of the life history, reproduction and phenology of Gracilaria[J]. Journal of Applied Phycology, 1995, 7(3):269-281.

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