深海嗜热异化铁还原菌<i>Caloranaerobacter ferrireducens</i> DY22619<sup>T</sup>对不同铁氧化物的铁还原特性

李曦; 曾湘; 张昭; 邵宗泽

doi:10.3969/j.issn.0253-4193.2016.08.009

深海嗜热异化铁还原菌Caloranaerobacter ferrireducens DY22619^T对不同铁氧化物的铁还原特性

doi: 10.3969/j.issn.0253-4193.2016.08.009

国家海洋局第三海洋研究所国家海洋局海洋生物遗传资源重点实验室, 福建厦门 361005;厦门市海洋生物遗传资源重点实验室(省部共建重点实培育基地), 福建厦门 361005;福建省海洋生物遗传资源重点实验室, 福建厦门 361005;福建省海洋生物资源开发利用协同创新中心, 福建厦门 361005

基金项目: 重点基础研究发展项目（973项目）（2012CB417304）；国际海域资源调查与开发“十二五”前沿性课题（DY125-22-QY-32）。

计量
- 文章访问数: 1259
- HTML全文浏览量: 22
- PDF下载量: 501
- 被引次数: 0
出版历程
- 收稿日期: 2015-11-10
- 修回日期: 2016-02-29

Characteristics of different iron oxides reduction by a thermophilic dissimilatory iron reducing bacterium Caloranaerobacter ferrireducens DY22619^T from deep sea

Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, China;Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, China

摘要

摘要: 异化铁还原微生物在铁元素的地球化学循环中具有重要意义。深海热液活动是大洋铁元素的重要来源，目前深海热液环境中铁代谢相关微生物研究很少。本文对一株分离自深海热液区的嗜热异化铁还原菌新种Caloranaerobacter ferrireducens DY22619^T的铁还原特性进行分析，比较了该菌对无定形羟基氧化铁、无定形铁氧化物和针铁矿3种不同铁氧化物的铁还原速率；并利用透射电镜对矿化产物进行矿物形貌、组成元素和晶型的分析。研究发现该菌生长在指数期至稳定期时，铁还原速率最快，其中对无定形羟基氧化铁和无定形铁氧化物的还原速率较高，达2.82 μmol/h和2.15 μmol/h；透射电镜结果表明，该菌可将3种不同胞外铁氧化物均还原矿化形成颗粒状磁铁矿，由针铁矿矿化形成的磁铁矿少但粒径最大，而由无定形铁氧化物形成的磁铁矿晶面不同于另外两种铁氧化物。结果表明，该菌有很强的铁还原和矿化能力，能厌氧呼吸还原三价铁氧化物，但是铁氧化物的性质对该菌胞外铁还原能力和矿化形成的磁铁矿的性质有重要影响。本研究为认识深海热液环境中异化铁还原菌在铁元素的地球化学循环和生物成矿过程提供了参考。
- Caloranaerobacterferrireducens /
- Fe(Ⅲ)还原 /
- 磁铁矿 /
- 生物成矿
Abstract: Dissimilatory iron reduction microbes play an important role in iron geochemical cycle. Deep-sea hydrothermal activity is an important source of the oceanic iron. At present there are little research about iron metabolism microbes in deep-sea hydrothermal areas. In this report, the iron reduction capability of Caloranaerobacter ferrireducens DY22619^T from deep sea was characterized. This article compared the iron reduction rate with three different iron oxides as electron acceptor, and analyzed the mineral morphology, element component and crystal face using Transmission Electron Microscope(TEM). The iron reduction rate was the fastest during from exponential to stationary phase and the rate with Amorphous FeOOH and Amorphous Fe(Ⅲ) Oxide was much higher reaching about 2.82 μmol/h and 2.15 μmol/h. TEM results showed the bacterium reduced all the three kinds of iron oxides and formed magnetite particles. The granule size of the magnetite formed with Goethite was the largest, but less in granule number. In addition, the crystal face derived from reducing Amorphous Fe(Ⅲ) Oxide was different from other two oxides. The results indicated the strain has a high potential in iron reducing Fe(Ⅲ) oxides and mineralization in anaerobic respiration. However, the properties of the magnetite and iron reduction rate is influenced by the character of iron oxides. These research indicated the role of dissimilatory iron reduction bacteria of this genus in iron geochemical cycle and biomineralization in deep sea hydrothermal areas.
- Caloranaerobacter ferrireducens /
- iron reduction /
- magnetite /
- biomineralization

HTML全文

参考文献(41)

Myers C R, Nealson K H. Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor[J]. Science, 1988, 240(4857):1319-1321.

Lovley D R. Dissimilatory Fe(Ⅲ) and Mn(Ⅳ) reduction[J]. Microbiology and Molecular Biology Reviews, 1991, 55(2):259-287.

李陛, 吴文芳, 李金华, 等. 温度和电子传递体AQDS对铁还原细菌Shewanella putrefaciens CN32矿化产物的影响[J]. 地球物理学报, 2011, 54(10):2631-2638. Li Bi, Wu Wenfang, Li Jinhua, et al. Effects of temperature on biomineralization of iron reducing bacteria Shewanella putrefaciens CN32[J]. Chinese Journal of Geophysics, 2011, 54(10):2631-2638.

欧阳冰洁, 陆现彩, 刘欢, 等. 希瓦氏奥奈达菌MR-1还原针铁矿的实验研究及地球化学意义[J]. 矿物学报, 2013, 33(3):389-396. Ouyang Bingjie, Lu Xiancai, Liu Huan, et al. Reduction of goethite by Shewanella oneidensis MR-1 and its geochemical implication[J]. Acta Mineralogica Sinica, 2013, 33(3):389-396.

Hekinian R, Hoffert M, Larque P, et al. Hydrothermal Fe and Si oxyhydroxide deposits from south Pacific intraplate volcanoes and East Pacific rise axial and off-axial regions[J]. Economic Geology, 1993, 88(8):2099-2121.

Slobodkina G B, Kolganova T V, Chernyh N A, et al. Deferribacter autotrophicus sp. nov., an iron(Ⅲ)-reducing bacterium from a deep-sea hydrothermal vent[J]. International Journal of Systematic and Evolutionary Microbiology, 2009, 59(6):1508-1512.

Miroshnichenko M L, Slobodkin A I, Kostrikina N A, et al. Deferribacter abyssi sp. nov., an anaerobic thermophile from deep-sea hydrothermal vents of the Mid-Atlantic Ridge[J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53(5):1637-1641.

Wery N, Lesongeur F, Pignet P, et al. Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent[J]. International Journal of Systematic and Evolutionary Microbiology, 2001, 51(2):495-504.

Lin T J, Breves E A, Dyar M D, et al. Magnetite formation from ferrihydrite by hyperthermophilic archaea from Endeavour Segment, Juan de Fuca Ridge hydrothermal vent chimneys[J]. Geobiology, 2014, 12(3):200-211.

Zeng Xiang, Zhang Zhao, Li Xi, et al. Caloranaerobacter ferrireducens sp. nov., an anaerobic, thermophilic, iron (Ⅲ)-reducing bacterium isolated from deep-sea hydrothermal sulfide deposits[J]. International Journal of Systematic and Evolutionary Microbiology, 2015, 65(6):1714-1718.

Lovley D R, Philips E J P. Novel mode of microbial energy metabolism:organic carbon oxidation coupled to dissimilatory reduction of iron or manganese[J]. Applied and Environmental Microbiology, 1988, 54(6):1472-1480.

Stookey L L. Ferrozine-a new spectrophotometric reagent for iron[J]. Analytical Chemistry, 1970, 42(7):779-781.

Lovley D R, Holmes D E, Nevin K P. Dissimilatory Fe(Ⅲ) and Mn(Ⅳ) reduction[J]. Advances in Microbial Physiology, 2004, 49:219-286.

Lehours A C, Rabiet M, Morel-Desrosiers N, et al. Ferric iron reduction by fermentative strain BS2 isolated from an iron-rich anoxic environment (Lake Pavin, France)[J]. Geomicrobiology Journal, 2010, 27(8):714-722.

Vecchia E D, Suvorova E I, Maillard J, et al. Fe(Ⅲ) reduction during pyruvate fermentation by Desulfotomaculum reducens strain MI-1[J]. Geobiology, 2014, 12(1):48-61.

Childers S E, Ciufo S, Lovley D R. Geobacter metallireducens accesses insoluble Fe(Ⅲ) oxide by chemotaxis[J]. Nature, 2002, 416(6882):767-769.

Gorby Y A, Yanina S, McLean J S, et al. Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(30):11358-11363.

Reguera G, McCarthy K D, Mehta T, et al. Extracellular electron transfer via microbial nanowires[J]. Nature, 2005, 435(7045):1098-1101.

Lovley D R, Coates J D, Blunt-Harris E L, et al. Humic substances as electron acceptors for microbial respiration[J]. Nature, 1996, 382(6590):445-448.

Nevin K P, Lovley D R. Mechanisms for accessing insoluble Fe(Ⅲ) oxide during dissimilatory Fe(Ⅲ) reduction by Geothrix fermentans[J]. Applied and Environmental Microbiology, 2002, 68(5):2294-2299.

Nevin K P, Lovley D R. Mechanisms for Fe(Ⅲ) oxide reduction in sedimentary environments[J]. Geomicrobiology Journal, 2002, 19(2):141-159.

Newman D K, Kolter R. A role for excreted quinones in extracellular electron transfer[J]. Nature, 2000, 405(6782):94-97.

Turick C E, Tisa L S, Caccavo Jr F. Melanin production and use as a soluble electron shuttle for Fe(Ⅲ) oxide reduction and as a terminal electron acceptor by Shewanella algae BrY[J]. Applied and Environmental Microbiology, 2002, 68(5):2436-2444.

Weber K A, Achenbach L A, Coates J D. Microorganisms pumping iron:anaerobic microbial iron oxidation and reduction[J]. Nature Reviews Microbiology, 2006, 4(10):752-764.

Hernandez M E, Kappler A, Newman D K. Phenazines and other redox-active antibiotics promote microbial mineral reduction[J]. Applied and Environmental Microbiology, 2004, 70(2):921-928.

Nevin K P, Lovley D R. Potential for nonenzymatic reduction of Fe(Ⅱ) via electron shuttling in subsurface sediments[J]. Environmental Science & Technology, 2000, 34(12):2472-2478.

张昭. 两株来自深海热液区的嗜热铁还原细菌的分离鉴定及其铁还原机制的研究[D]. 厦门:厦门大学, 2013. Zhang Zhao. Isolation and characterization of two thermophilic iron-reducing bacteria from deep sea hydrothermal vents and their iron-reducing mechanisms[D]. Xiamen:Xiamen University, 2013.

Salas E C, Berelson W M, Hammond D E, et al. The impact of bacterial strain on the products of dissimilatory iron reduction[J]. Geochimica et Cosmochimica Acta, 2010, 74(2):574-583.

Roh Y, Moon H S. Iron reduction by a psychrotolerant Fe(Ⅲ)-reducing bacterium isolated from ocean sediment[J]. Geosciences Journal, 2001, 5(3):183-190.

Roh Y H, Gao Haichun, Vali D, et al. Metal reduction and iron biomineralization by a psychrotolerant Fe(Ⅲ)-reducing bacterium, Shewanella sp. strain PV-4[J]. Applied and Environmental Microbiology, 2006, 72(5):3236-3244.

Lin T J. Microbe-Mineral relationships and biogenic mineral transformations in actively venting deep-sea hydrothermal sulfide chimneys[D]. Massachusetts:University of Massachusetts-Amherst, 2014.

Kashefi K, Tor J M, Holmes D E, et al. Geoglobus ahangari gen. nov., sp. nov., a novel hyperthermophilic archaeon capable of oxidizing organic acids and growing autotrophically on hydrogen with Fe(Ⅲ) serving as the sole electron acceptor[J]. International Journal of Systematic and Evolutionary Microbiology, 2002, 52(3):719-728.

Brügger K, Chen Lanming, Stark M, et al. The genome of Hyperthermus butylicus:a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108℃[J]. Archaea, 2007, 2(2):127-135.

Zillig W, Holz I, Janekovic D, et al. Hyperthermus butylicus, a hyperthermophilic sulfur-reducing archaebacterium that ferments peptides[J]. Journal of Bacteriology, 1990, 172(7):3959-3965.

Slobodkin A, Campbell B, Cary S C, et al. Evidence for the presence of thermophilic Fe(Ⅲ)-reducing microorganisms in deep-sea hydrothermal vents at 13 degrees N (East Pacific Rise)[J]. FEMS Microbiology Ecology, 2001, 36(2/3):235-243.

Huber H, Thomm M, König H, et al. Methanococcus thermolithotrophicus, a novel thermophilic lithotrophic methanogen[J]. Archives of Microbiology, 1982, 132(1):47-50.

Xiao Xiang, Wang Peng, Zeng Xiang, et al. Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment[J]. International Journal of Systematic and Evolutionary Microbiology, 2007, 57(1):60-65.

Bale S J, Goodman K, Rochelle P A, et al. Desulfovibrio profundus sp. nov., a novel barophilic sulfate-reducing bacterium from deep sediment layers in the Japan Sea[J]. International Journal of Systematic Bacteriology, 1997, 47(2):515-521.

Holmes D E, Bond D R, Lovley D R. Electron transfer by Desulfobulbus propionicus to Fe(Ⅲ) and graphite electrodes[J]. Applied and Environmental Microbiology, 2004, 70(2):1234-1237.

Pfennig N, Biebl H. Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium[J]. Archives of Microbiology, 1976, 110(1):3-12.

Roden E E, Lovley D R. Dissimilatory Fe(Ⅲ) reduction by the marine microorganism Desulfuromonas acetoxidans[J]. Applied and Environmental Microbiology, 1993, 59(3):734-742.

施引文献

资源附件(0)

访问统计