基于形态和16S rRNA基因的拟相手蟹分类研究

杨明柳; 徐敬明; 吴斌; 阎冰

doi:10.3969/j.issn.0253-4193.2016.08.007

基于形态和16S rRNA基因的拟相手蟹分类研究

doi: 10.3969/j.issn.0253-4193.2016.08.007

1.
广西科学院广西红树林研究中心广西红树林保护与利用重点实验室, 广西北海 536000
2.
重庆文理学院林学与生命科学学院, 重庆永川 402168

基金项目: 广西红树林保护与利用重点实验室基金（GKLMC-201205）；“红树林和海草生态系统保育与生态监测”特聘专家岗位专项。

计量
- 文章访问数: 1018
- HTML全文浏览量: 13
- PDF下载量: 485
- 被引次数: 0
出版历程
- 收稿日期: 2015-11-12
- 修回日期: 2016-03-13

Taxonomic study on the genus Parasesarma based on the morphology characteristics and mtDNA 16S rRNA gene

1.
Guangxi Key Laboratory for Mangrove Conservation and Utilization, Guangxi Academy of Sciences, Guangxi Mangrove Research Center, Beihai 536000, China
2.
College of Forestry and Life Science, Chongqing University of Arts and Sciences, Yongchuan 402168, China

摘要

摘要: 拟相手蟹属因其形态极其相似成为相手蟹科分类中最有疑问的一个属。通过对中国沿海近亲拟相手蟹Parasesarma affine、斑点拟相手蟹P. pictum、三栉拟相手蟹P. tripectinis、P. ungulatum及褶痕拟相手蟹P. plicatum 5种拟相手蟹的形态对比发现，可从它们的螯足形状(包括螯足掌节背面的梳状栉，可动指背面突起数目)及雄性第一腹肢形状对其进行区分。对其线粒体16S rRNA基因序列进行分子系统发育分析，结果表明5种拟相手蟹之间的遗传距离为1.9%～9.3%，达到了种间差异水平；构建的系统发育树显示近亲拟相手蟹与褶痕拟相手蟹汇聚成一支，随后与P. ungulatum聚在一起，斑点拟相手蟹与三栉拟相手蟹汇聚成独立支系。形态和分子证据均支持5种拟相手蟹分别为独立有效物种。
- 拟相手蟹 /
- 形态特征 /
- 16SrRNA基因 /
- 分类研究
Abstract: The taxonomy of the genus Parasesarma is one of the most problematic in the Sesarmidae, with their very similar morphology. We compared the morphology characteristics of Parasesarma affine, P. pictum, P. tripectinis, P. ungulatum(from the coast of China) and P. plicatum(from Singapore), and found that these five species could be distinguished from the shape of chelipeds(which included pectinate crest of dorsal surface of palm, the number of dactylar tubercles of the chelipeds)and the male first gonopod. The partial sequences of mitochondrial DNA 16S rRNA gene of these five Parasesarma species were contributed to molecular phylogenetic analysis, and the result showed that the genetic distances was 1.9%~9.3% between interspecies, and had reached the level difference of interspecies; the phylogenetic tree indicated that P. affine and P. plicatum were clustered together and then clustered into a clade with P. ungulatum, P. pictum and P. tripectinis were clustered into a distinct clade. Both of evidence of morphological and molecular data supported that the five Parasesarma species were separate and valid species.
- Parasesarma /
- morphology characteristics /
- 16S rRNA gene /
- taxonomic study

HTML全文

参考文献(22)

Ng P K L, Guinot D, Davie P J F. Systema Brachyurorum:Part Ⅰ. An annotated checklist of extant brachyuran crabs of the world[J]. The Raffles Bulletin of Zoology, 2008(S17):1-286.

Davie P J F, Pabriks L. A new species of Parasesarma(Crustacea:Brachyura:Sesarmidae) from the mangroves of Western Australia[J]. Zootaxa, 2010, 2564:62-68.

Koller P, Liu H C, Schubart C D. A new semiterrestrial species of Parasesarma De Man, 1895, from Taiwan (Decapoda, Brachyura, Sesarmidae)[M]//Studies on Malacostraca:Lipke Bijdeley Holthuis Memorial Volume. Crustaceana Monographs, 2011, 14:357-368.

戴爱云, 杨思谅, 宋玉枝, 等. 中国海洋蟹类[M]. 北京:海洋出版社, 1986:482-491. Dai Aiyun, Yang Siliang, Song Yuzhi, et al. Crabs of the China Seas[M]. Beijing:China Ocean Press, 1986:482-491.

Rahayu D L, Ng P K L. Two new species of Parasesarma De Man, 1895, from Southeast Asia (Crustacea:Decapoda:Brachyura:Sesarmidae)[J]. Zootaxa, 2009, 1980:29-40.

Naderloo R, Schubart C D. Description of a new species of Parasesarma(Crustacea; Decapoda; Brachyura; Sesarmidae) from the Persian Gulf, based on morphological and genetic characteristics[J]. Zoologischer Anzeiger-A Journal of Comparative Zoology, 2010, 249(1):33-43.

刘瑞玉. 中国海洋生物名录[M]. 北京:科学出版社, 2008:772-809. Liu Ruiyu. Checklist of Marine Biota of China Seas[M]. Beijing:Science Press, 2008:772-809.

Rahayu D L, Ng P K L. Revision of the Parasesarma plicatum(Latreille, 1803) species-group (Crustacea:Decapoda:Brachyura:Sesarmidae)[J]. Zootaxa, 2010, 2327:1-22.

戴爱云, 宋玉枝. 广西北部湾潮间带蟹类的初步研究[G]//《甲壳动物学论文集》编辑委员会. 甲壳动物学论文集. 北京:科学出版社, 1986:54-62. Dai Aiyun, Song Yuzhi. Intertidal crabs from Beibu Gulf of Guangxi[C]//Editorial Committee of Symposium on Crustacea. Symposium on Crustacean Research in China. Beijing:Science Press, 1986:54-62.

Shen C J. Four new species of Brachyura from Chinese seas[J]. Journal of the Hong Kong Fisheries Research Station, 1940, 1(2):255-262.

Schubart C D, Cannicci S, Vannini M, et al. Molecular phylogeny of grapsoid crabs (Decapoda, Brachyura) and allies based on two mitochondrial genes and a proposal for refraining from current superfamily classification[J]. Journal of Zoological Systematics and Evolutionary Research, 2006, 44(3):193-199.

Shih H T, Ng P K L, Liu M Y. Systematics of the Indo-West Pacific broad-fronted fiddler crabs (Crustacea:Ocypodidae:genus Uca)[J]. The Raffles Bulletin of Zoology, 2013, 61(2):641-649.

Palumbi S R. Genetic divergence, reproductive isolation, and marine speciation[J]. Annual Review of Ecology and Systematics, 1994, 25:547-572.

Swofford D L. PAUP*:phylogenetic analysis using parsimony, version 4. 0 b10[Z]. Sunderland, MA, USA:Sinauer Associates, 2003.

Davie P J F. A new species of Sesarmine crab (Brachyura:Grapsidae) from Japan and Taiwan, previously known as Sesarma erythodactyla Hess, 1865[J]. Crust Res, 1993, 22:65-74.

Ng P K L, Wang C H, Ho P H, et al. An annotated checklist of brachyuran crabs from Taiwan (Crustacea:Decapoda)[M]//National Taiwan Museum Special Publication Series, No. 11. Taipei, Taiwan, China:National Taiwan Museum, 2001:1-86.

Milne-Edwards H. Mémoire sur la famille des Ocypodiens[G]//Annales des Sciences Naturelles (Zoologie) Series 3. 1853, 20:163-228.

Yeo D C J, Shih H T, Meier R, et al. Phylogeny and biogeography of the freshwater crab genus Johora (Crustacea:Brachyura:Potamidae) from the Malay Peninsula, and the origins of its insular fauna[J]. Zoologica Scripta, 2007, 36(3):255-269.

Shih H T, Ng P K L, Schubart C D, et al. Phylogeny and phylogeography of the genus Geothelphusa(Crustacea:Decapoda, Brachyura, Potamidae) in southwestern Taiwan based on two mitochondrial genes[J]. Zoological Science, 2007, 24(1):57-66.

Shih H T, Suzuki H. Taxonomy, phylogeny, and biogeography of the endemic mudflat crab Helice/Chasmagnathus complex (Crustacea:Brachyura:Varunidae) from East Asia[J]. Zoological Studies, 2008, 47(1):114-125.

徐敬明, 孙翰昌, 孙世春. 厚蟹线粒体16S rRNA基因序列分析及系统发育研究[J]. 海洋学报, 2011, 33(5):124-132. Xu Jingming, Sun Hanchang, Sun Shichun. Molecular phylogeny of the crab genera Helice and Helicana(Crustacea, Decapoda) based on the partial sequences of mitochondrial 16S rRNA gene[J]. Haiyang Xuebao, 2011, 33(5):124-132.

Schubart C D, Koller P. Genetic diversity of freshwater crabs (Brachyura:Sesarmidae) from central Jamaica with description of a new species[J]. Journal of Natural History, 2005, 39(6):469-481.

施引文献

资源附件(0)

访问统计