日本囊对虾<i>Marsupenaeus japonicus</i>)组织蛋白酶D基因克隆及表达分析

张曼; 程光平; 苏永全; 徐伊桦; 冯文荣; 王军; 宋晓红; 毛勇

doi:10.3969/j.issn.0253-4193.2016.08.006

日本囊对虾Marsupenaeus japonicus)组织蛋白酶D基因克隆及表达分析

doi: 10.3969/j.issn.0253-4193.2016.08.006

1.
广西大学动物科学技术学院广西高校水生生物健康养殖与营养调控重点实验室, 广西南宁 530005;厦门大学海洋与地球学院, 福建厦门 361005
2.
广西大学动物科学技术学院广西高校水生生物健康养殖与营养调控重点实验室, 广西南宁 530005
3.
厦门大学海洋与地球学院, 福建厦门 361005

基金项目: 国家现代农业虾产业技术体系专项（CARS-47）；广西自然科学基金项目（2015GXNSFBA139066）。

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出版历程
- 收稿日期: 2015-12-15
- 修回日期: 2016-03-31

Molecular cloning and expression analysis of cathepsin D from kuruma shrimp (Marsupenaeus japonicus)

1.
Guangxi Colleges and Universities Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation, College of Animal Science and Technology, Guangxi University, Nanning 530005, China;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
2.
Guangxi Colleges and Universities Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation, College of Animal Science and Technology, Guangxi University, Nanning 530005, China
3.
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China

摘要

摘要: 组织蛋白酶D是溶酶体天冬氨酸蛋白酶家族的主要成员，广泛参与动物机体细胞内蛋白质的降解过程，对维持细胞稳态和正常代谢具有重要的作用。为研究组织蛋白酶D在甲壳动物非特异性免疫和幼体发育过程中的作用，本研究采用RACE技术首次克隆得到日本囊对虾组织蛋白酶D基因cDNA序列，命名为MjCatD，其中开放阅读框长为1161 bp，编码386个氨基酸残基。序列分析和同源建模显示该基因编码的蛋白含有保守的N-糖基化位点、天冬氨酸蛋白酶签名序列、酶活化位点和非消化性组织蛋白酶D的特征序列，并且呈保守的双叶形结构。同源性比较和系统进化分析发现，MjCatD与斑节对虾、美洲螯龙虾和脊尾白虾相似性较高，并且与它们紧密聚为一支。实时荧光定量PCR结果显示，MjCatD基因在日本囊对虾多个组织中均有表达，其中肝胰腺中表达量最高。在白斑综合征病毒(white spot syndrome virus, WSSV)感染后3～24 h日本囊对虾肝胰腺中MjCatD的表达量逐渐下降，而在48 h急剧上调至最高表达量并且与对照组差异极显著(P<0.01)。此外，MjCatD基因在幼体发育不同阶段中也表现出明显的变化趋势。以上研究表明，MjCatD基因可能参与日本囊对虾先天免疫反应和幼体发育过程。
- 日本囊对虾 /
- 组织蛋白酶D /
- 基因克隆 /
- 基因表达
Abstract: Cathepsin D is the principal member of lysosomal aspartic proteinase family which participates in various degradations of intracellular protein and plays important roles in normal metabolism for the maintenance of cellular homeostasis. In this current study, the cDNA sequence of kuruma shrimp (Marsupenaeus japonicus) cathepsin D (MjCatD) was cloned for the first time through RACE technology, including a 1 161 bp open reading frame encoding 386 amino acids. Both sequence analysis and homology modeling revealed that the deduced protein of MjCatD contained well conserved N-glycosylation site, aspartic proteinase signature sequence, active site and the characteristic sequence of non-digestive cathepsin D, as well as presented a conserved bilobal structure. MjCatD shared high similarity with those from Penaeus monodon, Homarus americanus, Palaemon carinicauda, and clustered together with above three cathepsin Ds. Real-time quantitative PCR demonstrated that MjCatD was ubiquitously expressed in all the examined tissues, predominantly in hepatopancreas. After challenging with white spot syndrome virus (WSSV), the expression level of MjCatD in the hepatopancreas was gradually down regulated during 3 h to 24 h, then very significantly increased (P<0.01) and peaked at 48 h compared to the control. MjCatD also showed obvious changes during different larval stages. These findings indicated that MjCatD may play key roles in the innate immune response and larval development of kuruma shrimp.
- Marsupenaeus japonicus /
- cathepsin D /
- gene cloning /
- gene expression

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参考文献(28)

Liu Haipeng, Söderhäll K, Jiravanichpaisal P. Antiviral immunity in crustaceans[J]. Fish & Shellfish Immunology, 2009, 27(2):79-88.

Honey K, Rudensky A Y. Lysosomal cysteine proteases regulate antigen presentation[J]. Nature Reviews Immunology, 2003, 3(6):472-482.

Hsing L C, Rudensky A Y. The lysosomal cysteine proteases in MHC class Ⅱ antigen presentation[J]. Immunological Reviews, 2005, 207(1):229-241.

Liaudet-Coopman E, Beaujouin M, Derocq D, et al. Cathepsin D:newly discovered functions of a long-standing aspartic protease in cancer and apoptosis[J]. Cancer Letters, 2006, 237(2):167-179.

曾广智, 谭宁华, 贾锐锐, 等. 组织蛋白酶及其抑制剂研究进展[J]. 云南植物研究, 2005, 27(4):337-354. Zeng Guangzhi, Tan Ninghua, Jia Ruirui, et al. Cathepsins:structures, functions and inhibitors[J]. Acta Botanica Yunnanica, 2005, 27(4):337-354.

张志林, 肖蓉, 李庆伟. 组织蛋白酶D的功能多样性[J]. 中国生物化学与分子生物学报, 2014, 30(7):647-654. Zhang Zhilin, Xiao Rong, Li Qingwei. Diversified functions of cathepsin D[J]. Chinese Journal of Biochemistry and Molecular Biology, 2014, 30(7):647-654.

Choi K M, Shim S H, An C M, et al. Cloning, characterisation, and expression analysis of the cathepsin D gene from rock bream (Oplegnathus fasciatus)[J]. Fish & Shellfish Immunology, 2014, 40(1):253-258.

Carnevali O, Centonze F, Brooks S, et al. Molecular cloning and expression of ovarian cathepsin D in seabream, Sparus aurata[J]. Biology of Reproduction, 1999, 61(3):785-791.

Dong Zhongdian, Zhang Jiao, Ji Xiangshan, et al. Molecular cloning, characterization and expression of cathepsin D from grass carp (Ctenopharyngodon idella)[J]. Fish & Shellfish Immunology, 2012, 33(5):1207-1214.

Rojo L, Sotelo-Mundo R, García-Carreño F, et al. Isolation, biochemical characterization, and molecular modeling of American lobster digestive cathepsin D1[J]. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 2010, 157(4):394-400.

段亚飞, 刘萍, 李吉涛, 等. 脊尾白虾(Exopalaemon carinicauda)组织蛋白酶D基因的克隆及其表达分析[J]. 海洋与湖沼, 2013, 44(3):599-605. Duan Yafei, Liu Ping, Li Jitao, et al. Cloning and expression of cathepsin D gene in Exopalaemon carinicauda[J]. Oceanologia et Limnologia Sinica, 2013, 44(3):599-605.

孙田田, 苏永全, 洪婧妮, 等. 真蛸热休克蛋白90基因(HSP90)的克隆及表达[J]. 水产学报, 2012, 36(9):1367-1375. Sun Tiantian, Su Yongquan, Hong Jingni, et al. Molecular cloning and feature analysis of heat shock protein 90(HSP90) from Octopus vulgaris[J]. Journal of Fisheries of China, 2012, 36(9):1367-1375.

Schwede T, Kopp J, Guex N, et al. SWISS-MODEL:an automated protein homology-modeling server[J]. Nucleic Acids Research, 2003, 31(13):3381-3385.

Xiao Rong, Zhang Zhilin, Wang Hongyan, et al. Identification and characterization of a cathepsin D homologue from lampreys (Lampetra japonica)[J]. Developmental & Comparative Immunology, 2015, 49(1):149-156.

Fortenberry S C, Schorey J S, Chirgwin J M. Role of glycosylation in the expression of human procathepsin D[J]. Journal of Cell Science, 1995, 108(5):2001-2006.

Margaryan N V, Kirschmann D A, Lipavsky A, et al. New insights into cathepsin D in mammary tissue development and remodeling[J]. Cancer Biology & Therapy, 2010, 10(5):457-466.

Masson O, Prébois C, Derocq D, et al. Cathepsin-D, a key protease in breast cancer, is up-regulated in obese mouse and human adipose tissue, and controls adipogenesis[J]. PLoS One, 2011, 6(2):e16452.

Gross P S, Bartlett T C, Browdy C L, et al. Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific White Shrimp, Litopenaeus vannamei, and the Atlantic White Shrimp, L. setiferus[J]. Developmental & Comparative Immunology, 2001, 25(7):565-577.

朱建中, 陆承平. 对虾白斑综合征病毒射阳株的分离及其在螯虾体内的动态分布[J]. 南京农业大学学报, 2002, 25(3):75-79. Zhu Jianzhong, Lu Chengping. Isolation and dynamic distribution of white spot syndrome virus Sheyang strain in crawfishes[J]. Journal of Nanjing Agricultural University, 2002, 25(3):75-79.

Chen I T, Aoki T, Huang Y T, et al. White spot syndrome virus induces metabolic changes resembling the warburg effect in shrimp hemocytes in the early stage of infection[J]. Journal of Virology, 2011, 85(24):12919-12928.

Chang P S, Lo C F, Wang Y C, et al. Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization[J]. Diseases of Aquatic Organisms, 1996, 27(2):131-139.

Zhang Man, Mao Yong, Wang Jun, et al. Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus)[J]. Acta Oceanologica Sinica, 2015, 34(2):74-83.

Zuzarte-Luis V, Montero J A, Kawakami Y, et al. Lysosomal cathepsins in embryonic programmed cell death[J]. Developmental Biology, 2007, 301(1):205-217.

Cho J H, Park I Y, Kim H S, et al. Cathepsin D produces antimicrobial peptide parasinⅠfrom histone H₂A in the skin mucosa of fish[J]. The FASEB Journal, 2002, 16(3):429-431.

Laubier-Bonichon A, Van Wormhoudt A, Sellos D. Croissance larvaire contrôlée de Penaeus japonicus Bate. Enzymes digestives et changements de régimes alimentaires[C]//Proceedings of the 3rd Meeting of the I.C.E.S. Working Group on Mariculture. Brest, France, 1977:131-145.

Lemos D, Hernández-Cortés M P, Navarrete A, et al. Ontogenetic variation in digestive proteinase activity of larvae and postlarvae of the pink shrimp Farfantepenaeus paulensis(Crustacea:Decapoda:Penaeidae)[J]. Marine Biology, 1999, 135(4):653-662.

Ribeiro F, Jones D. Growth and ontogenetic change in activities of digestive enzymes in Fennero Penaeus indicus postlarvae[J]. Aquaculture Nutrition, 2000, 6(1):53-64.

Mourente G, Medina A, González S, et al. Variations in lipid content and nutritional status during larval development of the marine shrimp Penaeus kerathurus[J]. Aquaculture, 1995, 130(2/3):187-199.

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