白缘(鱼央)5S rDNA的扩增分析及其在染色体上的FISH定位

张伟伟; 付元帅; 邵燕; 王宝琴; 石东里; 张兰

doi:10.3969/j.issn.0253-4193.2016.06.009

白缘(鱼央)5S rDNA的扩增分析及其在染色体上的FISH定位

doi: 10.3969/j.issn.0253-4193.2016.06.009

1.
滨州学院山东省黄河三角洲野生植物资源开发利用工程技术研究中心, 山东滨州 256603
2.
上海海洋大学水产与生命学院 , 上海 201306

基金项目: 国家自然科学基金项目(31400401);山东省自然科学基金项目(ZR2013EML002);山东省科技发展计划项目(2011YD21020)。

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出版历程
- 收稿日期: 2015-06-03

The amplification and FISH analysis of 5S rDNA in Liobagrus marginatus

1.
Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou University, Binzhou 256603, China
2.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

摘要

摘要: 本文分别对雌雄白缘(鱼央)的5S rDNA进行了PCR扩增和序列分析,并采用双色荧光原位杂交(FISH)技术,以白缘(鱼央)的5S rDNA序列和小麦的45S rDNA为探针,对其在白缘(鱼央)雌雄中期染色体上进行了FISH定位研究。结果表明,白缘(鱼央)5S rDNA序列无雌雄差异;5S rDNA的保守区序列为117 bp;5S rDNA和45S rDNA分别被定位于白缘(鱼央)的性染色体和第5号染色体上。同时从GenBank中获得了22种鱼的5S rDNA,运用DNAman软件构建了23种鱼的系统发育树,对白缘(鱼央)的进化地位进行了初步研究。本研究为阐明白缘(鱼央)在鱼类系统进化中的位置、重复序列在脊椎动物性染色体上的分布状况以及与性别决定与分化的关系,提供了资料积累和分析依据。
- Liobagrusmarginatus /
- PCR /
- 5SrDNA /
- 系统进化树 /
- 染色体 /
- FISH
Abstract: In present research,5S rDNA was amplified by the usual 5S rDNA primers in Genomic DNA of female and male Liobagrus marginatus,and sequenced. The results of amplification showed that there were not different in 5S rDNA between female and male of L.marginatus,and length of 5S rDNA was 117 bp. Using the method of double color Fluorescence In situ Hybridization (FISH),5S rDNA and 45S rDNA on metaphase chromosomes were located in L.marginatus. The results showed that 5S rDNA and 45S rDNA were located on sex chromosome and the 5th chromosome,respectively. Phylogenetic tree of 5S rDNA in L.marginatus and 22 fishes else was constructed by DNAman,so evolution position of L.marginatus in fishes was studied preliminarily. This study offer data accumulating and basis of analysis,to illustrate phyletic evolution of fishes and repeat sequences on sex chromosome,and relation of heterochromatin or repeat sequences and sex determination & differentiation in vertebrates.
- Liobagrus marginatus /
- PCR /
- 5S rDNA /
- phylogenetic tree /
- chromosome /
- FISH

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参考文献(20)

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