热液和冷泉10种无脊椎动物基因组大小测定及比较

郑平; 王敏晓; 李超伦; 孙松

doi:10.3969/j.issn.0253-4193.2016.06.005

热液和冷泉10种无脊椎动物基因组大小测定及比较

doi: 10.3969/j.issn.0253-4193.2016.06.005

1.
中国科学院海洋研究所海洋生态与环境科学重点实验室, 山东青岛 266071;中国科学院大学, 北京 100049
2.
中国科学院海洋研究所海洋生态与环境科学重点实验室, 山东青岛 266071

基金项目: 中国科学院创新国际团队项目(20140491526);中国科学院战略性先导科技专项(A类)资助(XDA11020305);国家基金委-山东省联合基金项目"海洋生态与环境科学"(U1406403);自然科学基金(41106133,41230963)。

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出版历程
- 收稿日期: 2015-12-24

Survey of genome size in 10 invertebrates from hydrothermal vent and cold seep

1.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China
2.
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: 基因组大小(或称C值)作为生物单倍体细胞中全套染色体的DNA总量,在一定程度上是恒定的,因而C值可以作为生物物种的一个特定参数。深海热液和冷泉为更好地理解C值与不同环境之间的关系提供了一个特征性的模型。本文采用流式细胞术,测定了来自热液和冷泉环境中的10种深海无脊椎动物的C值,其分布范围从0.87 pg到12.28 pg,其中,相比于软体动物和多毛类,甲壳生物基因组大小及变异均较大。对比热液和冷泉两个群落中共有种(深海偏顶蛤Bathymodiolus platifrons、柯氏潜铠虾Shinkaia crosnieri以及长角阿尔文虾Alvinocaris longirostris)的基因组大小,发现C值差异并不显著。同时,综合已有的数据,对深海化能极端环境与其他环境条件下的物种C值进行对比分析,结果显示深海化能极端环境下生物的基因组大小并没有发现明确的变化趋势。
- 热液 /
- 冷泉 /
- 化能合成生态系统 /
- 基因组大小 /
- C值 /
- 流式细胞术
Abstract: Knowledge of genome size is an important prerequisite for the development of many genomic resources. To better understand the evolution of genome size and their roles in environmental adaption, it is necessary to collect data covering a broad taxonomic base, especially from particular ecosystems. With high disturbance and unique environmental features, hydrothermal vent and cold seep offer a typical model to investigate the variation of genome size under extreme environments. Here we measured 10 deep-sea invertebrates from hydrothermal vent and cold seep by flow cytometry. The C-value ranged from 0.87 pg to 12.28 pg, and the crustaceans have larger genomes. Then we compared the three common species (Bathymodiolus platifrons, Shinkaia crosnieri and Alvinocaris longirostris) in both environments. Though with high level of intraspecific variations, no significant differences were revealed between two populations. We also compared the obtained genome sizes to their corresponding taxonomic relatives (at family or order level). No evidence of genome size expansion or reduction were found in invertebrates from chemosynthetic environments. In our study, there are no clear trends of the genome size variations under the extreme deep-sea ecological conditions.
- hydrothermal vent /
- cold seep /
- chemosynthetic system /
- genome size /
- C-value /
- flow cytometry

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