羟基自由基杀灭压载水中有害赤潮物种的生物有效性研究

程超; 白敏冬; 郑琦琳; 陈操; 孟祥盈; 张芝涛

doi:10.3969/j.issn.0253-4193.2016.02.013

羟基自由基杀灭压载水中有害赤潮物种的生物有效性研究

doi: 10.3969/j.issn.0253-4193.2016.02.013

1.
大连海事大学轮机工程学院, 辽宁大连 116026
2.
厦门大学环境与生态学院, 福建厦门 361005;大连海事大学环境工程研究所, 辽宁大连 116026
3.
厦门大学环境与生态学院, 福建厦门 361005
4.
大连海事大学环境工程研究所, 辽宁大连 116026

基金项目: 国家科技支撑计划(2013BAC06B01,2013BAC06B02);国家重大科研仪器研制项目(NSFC 61427804);国家杰出青年科学基金(NSFC 61025001);海洋科学研究公共利益的专项基金。

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出版历程
- 收稿日期: 2015-06-09
- 修回日期: 2015-09-23

Research on biological effectiveness of hydroxyl radicals killing marine red tide algae in ballast water

1.
College of Marine Engineering, Dalian Maritime University, Dalian 116026, China
2.
College of Environment and Ecology, Xiamen University, Xiamen 361005, China;Environmental Engineering Institute, Dalian Maritime University, Dalian 116026, China
3.
College of Environment and Ecology, Xiamen University, Xiamen 361005, China
4.
Environmental Engineering Institute, Dalian Maritime University, Dalian 116026, China

摘要

摘要: 本研究采用大气压下强电离放电协同气液混溶技术,高效制备羟基自由基(·OH)杀灭3个门的典型有害赤潮物种,使用荧光染色、测定光合作用潜能等生物学检测方法确定·OH致死阈值。结果表明,5.05×10⁴ cells/mL的赤潮异弯藻(Heterosigma akashiwo)、5.28×10⁴ cells/mL的亚历山大藻(Alexandrium tamarense)和5.02×10⁴ cells/mL的中肋骨条藻(Skeletonema costatum),其致死阈值分别为1.24 mg/L、2.01 mg/L、1.12 mg/L,此时其叶绿素a分解率分别为77%、85%和74%。利用光学显微镜观察,处理前后藻细胞结构有明显的改变。因此,·OH致死方法可有效地杀灭压载水中的有害赤潮藻。
- 羟基自由基 /
- 赤潮藻 /
- 致死阈值
Abstract: In this study, the three typical red tide species were chosen as the experiment algae and the inactivation was achieved by hydroxyl radials generated from a strong ionization discharge combined with hydrodynamic cavitations. The viability and integrity of the algae were determined by the fluorescence staining and Pulse Amplitude Modulation. The results suggest that a quick and complete loss of viability was achieved for three species after exposure to hydroxyl radical, for the Heterosigma akashiwo, Alexandrium tamarensem, Skelrtonema costatum which the density is 5.05×10⁴ cells/mL, 5.28×10⁴ cells/mL, 5.02×10⁴ cells/mL respectively, the lethal thresholds are 1.24 mg/L, 2.01mg/L, and 1.12 mg/L separately. Meanwhile Algal cells were deformed and shrunk after ·OH attack and chlorophyll content was degraded at the same time. The chlorophyll content decomposition rate reaches to 77%, 85% and 74% at the lethal thresholds. Above all, the use of hydroxyl radicals is an efficient method to kill red tide species in ballast water.
- hydroxyl radials /
- red tide algae /
- lethal thresholds

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