不同粒径TiO<sub>2</sub>颗粒对海洋微藻的毒性效应

马菲菲; 孙雪梅; 韩倩; 陈碧鹃; 夏斌; 曲克明

doi:10.3969/j.issn.0253-4193.2015.10.010

不同粒径TiO₂颗粒对海洋微藻的毒性效应

doi: 10.3969/j.issn.0253-4193.2015.10.010

中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室山东省渔业资源与生态环境重点实验室, 山东青岛 266071

基金项目: 中国水产科学研究院基本科研业务费(2013A02YQ02);国家自然科学青年基金(41206100);中国水产科学研究院黄海水产研究所基本科研业务费专项基金(20603022012020)。

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- 收稿日期: 2015-01-05

Toxic effects of TiO₂ particles with different size on the marine microalga

Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Ecological Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

摘要

摘要: 本实验以新月菱形藻为受试生物,研究了低浓度不同粒径TiO₂颗粒(21 nm、60 nm和400 nm)对海洋微藻生长、抗氧化酶活性(超氧化物歧化酶SOD、过氧化氢酶CAT和过氧化物酶POD)、脂质过氧化产物(MDA)含量的影响,并测定了相应的活性氧自由基(ROS)的含量,初步探讨了TiO₂颗粒对海洋微藻的作用机制。结果表明,1 mg/L TiO₂颗粒对新月菱形藻生长的抑制作用随着粒径的减小而逐渐增强,第48 h、72 h、96 h呈现出显著的纳米效应。TiO₂颗粒可以诱导藻细胞内ROS的含量增加,对藻细胞产生氧化胁迫,新月菱形藻的抗氧化酶活性发生应激响应,以清除过量的ROS,但剩余的ROS对藻细胞产生氧化损伤,导致MDA含量升高,并且纳米级TiO₂颗粒对新月菱形藻的氧化损伤大于微米级颗粒。在不同粒径TiO₂颗粒的胁迫下,藻细胞SOD和CAT活性的响应也存在差异。本研究将为开展人工纳米材料对海洋生态系统影响的潜在风险评估提供科学依据。
- TiO₂ /
- 新月菱形藻 /
- 纳米颗粒 /
- 海洋微藻 /
- 抗氧化酶 /
- 毒性效应
Abstract: With the increasing application of nanotechnologies,it is no doubt that more and more engineered nanoparticles end up in marine environment. The toxicity of TiO₂ particles with different sizes (21 nm,60 nm and 400 nm) to the marine microalga Nitzschia closterium was investigated in this study,including the growth inhibition and oxidative stress. The results showed that the toxicity of 1 mg/L TiO₂ particles to N. closterium cells increased with the decrease of particle size,indicating significant nano-effects at 48 h,72 h and 96h exposure,respectively. TiO₂ particles induced excessive reactive oxygen species (ROS) which led to oxidative stress on the algal cells. The antioxidant enzyme activities of N. closterium cells could eliminate some ROS in order to protect algae cells. However,the rest could cause oxidative damage and increase malondialdehyde (MDA) levels concomitantly. In addition,the oxidative damage caused by nano-sized TiO₂ particles (21nm and 60nm) was greater than micro-sized particles. Superoxide dismutase (SOD) and catalase (CAT) activities of the algal cells had different response under the stress of TiO₂ particle with different size. This study shed new light on the assessment of ecological toxicity of engineered nanomaterials.
- TiO₂ /
- Nitzschia closterium /
- nanoparticles /
- marine microalga /
- antioxidant enzyme /
- toxic effect

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