光强和二氧化碳浓度变化对浒苔幼苗生长及生理的影响

高秀秀; 李亚鹤; 段维军; 徐年军

doi:10.3969/j.issn.0253-4193.2015.10.008

光强和二氧化碳浓度变化对浒苔幼苗生长及生理的影响

doi: 10.3969/j.issn.0253-4193.2015.10.008

1.
宁波大学应用海洋生物技术教育部重点实验室, 浙江宁波 315211;浙江省海洋生物工程重点实验室, 浙江宁波 315211
2.
宁波出入境检验检疫局技术中心, 浙江宁波 315012

基金项目: 国家自然科学基金项目(40876073, 41276122);国家教育部博士点基金博导基金(20123305110002); 宁波大学学科项目(XKL14D2085);宁波大学科研启动项目(F01259144702)。

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出版历程
- 收稿日期: 2015-02-11

The effects of light and increased CO₂ on the growth and physiological performances in marine green algae Ulva prolifera seedling

1.
Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China;Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China
2.
Ningbo Entry-Exit Inspection and Quarantine Bureau Technology Centre, Ningbo 315012, China

摘要

摘要: 大气CO₂浓度升高引起的海洋酸化如何在光变环境下影响大型海藻固碳量的问题,关系到未来海洋初级生产力的变化趋势。为研究大型海藻对CO₂浓度升高和光强变化的响应,本文选取浒苔(Ulva prolifera)幼苗为实验材料,探讨其在不同光强下[80、260 μmol/(m²·s)]和两种CO₂浓度(正常CO₂浓度:400 μL/L和高CO₂浓度:1 000 μL/L)下的生理变化。研究发现,在正常CO₂浓度、高光条件下,浒苔幼苗的生长最快,超氧化物特化酶(SOD)活性最高,而过氧化氢酶(CAT)活性在低光、高CO₂处理下有最大值。光合色素含量和光系统Ⅱ的光化学效率在不同处理间没有显著性差异,但叶绿素a与类胡萝卜素的比值在低光正常CO₂处理下有最大值。同时,高光高CO₂处理下,浒苔幼苗的可溶性蛋白含量最低。
- CO₂ /
- 光强 /
- 浒苔幼苗 /
- 叶绿素荧光效率 /
- 抗氧化活性
Abstract: Ocean acidification due to increasing anthropogenic CO₂ is expected to influence marine primary productivity. In order to investigate the interactive effects of increased light and CO₂ changes on macroalgae,we grew Ulva prolifera seedling under the normal CO₂ level (400 μL/L) and elevated CO₂ level (1 000 μL/L) conditions,the light levels set as 80 and 260 μmol/(m²·s),and measured its physiological performance. Research finding that the specific growth rate and activity of SOD was highest under high light and normal CO₂ level . However,the activity of CAT showed the highest value under the high CO₂ and low light conditions. Under the different treatments,there was no significant effect on the chlorophyll a and maximal photochemical yield of PSⅡ (F_v/F_m),while the largest ratios of chlorophylla to carotenoid occurred at nomal CO₂ and low light conditions. Meanwhile,the content of soluble protein was decreased by high light and high CO₂.
- CO₂ /
- light /
- Ulva prolifera seedling /
- chlorophyll fluorescence efficiency /
- Antioxidant activity

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