海水酸化对中华哲水蚤全蛋白表达的影响

张达娟; 郭东晖; 王桂忠; 李少菁

doi:10.3969/j.issn.0253-4193.2015.06.010

海水酸化对中华哲水蚤全蛋白表达的影响

doi: 10.3969/j.issn.0253-4193.2015.06.010

1.
天津农学院水产学院天津市水产生态及养殖重点实验室, 天津 300384
2.
厦门大学海洋与地球学院, 福建厦门 361005

基金项目: 国家自然科学基金"不同生态类型的海洋桡足类对硅藻的利用与适应的生态遗传学研究"面上项目(41276132)。

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出版历程
- 收稿日期: 2014-09-23

Proteomic study of the effects of acidified seawater on Calanus sinicus

1.
College of Fisheries, Tianjin Agricultural College, Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Tianjin 300384, China
2.
College of Ocean & Earth Science, Xiamen University, Xiamen 361005, China

摘要

摘要: 运用蛋白质组学相关技术对暴露于酸化海水中的中华哲水蚤全蛋白进行分析,结果表明,对照组、C_CO₂ 0.08%和C_CO₂ 0.20%处理组中华哲水蚤的双向电泳图谱上可以分别分辨出1 191、1 117和946个蛋白点,选取其中43个差异蛋白进行MALDI-TOF/TOF质谱鉴定,成功鉴定出23个差异蛋白,这些蛋白主要与蛋白合成和分解、能量代谢、DNA分子修复以及解毒过程有关。
- 二氧化碳 /
- 酸化 /
- 中华哲水蚤 /
- 蛋白质组学
Abstract: This study compared proteim profiles of Calanus sinicus cultured under C_CO₂ 0.08% and C_CO₂ 0.20% CO₂-acidified seawater for 4 days using a proteomic approach, and identified the differentially expressed proteins. The results are shown that, 1 191, 1 117 and 946 protein spots of C.sinicus in control, C_CO₂ 0.08% and C_CO₂ 0.20% groups were detected in the two-dimensional electrophoresis gels, respectively. The 43 protein spots were selected based on their differential expression between control and C_CO₂ 0.08% group, C_CO₂ 0.20% group, and 23 proteins of which were identified by MALDI-TOF/TOF mass spectrometry. The data demonstrated that these differentially expressed proteins were associated with protein synthesize and proteolysis, energy metabolism, DNA repaired and detoxified of organisms.
- carbon dioxide /
- ocean acidification /
- Calanus sinicus /
- proteome

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Scheffer M, Carpenter S, Foley J A, et al. Catastrophic shifts in ecosystems[J]. Nature, 2001, 413(6856): 591-596.

IPCC. A contribution of Working Groups Ⅰ, Ⅱ and Ⅲ to the third assessment report of the intergovernmental panel on climate change[M]. Cambridge: Cambridge University Press, 2001.

Royal Society. Ocean acidification due to increasing atmospheric carbon dioxide[M]. London: The Royal Society, 2005: 60.

Clark D, Lamare M, Barker M. Response of sea urchin pluteus larvae (Echinodermata: Echinoidea) to reduced seawater pH: a comparison among a tropical, temperate, and a polar species[J]. Marine Biology, 2009, 156(6): 1125-1137.

Zippay M L, Hofmann G E. Effect of pH on gene expression and thermal tolerance of early life history stages of red abalone (Haliotis rufescens)[J]. Journal of Shellfish Research, 2010, 29(2): 429-439.

Bradassi F, Cumani F, Bressan G, et al. Early reproductive stages in the crustose coralline alga phymatolithon lenormandii are strongly affected by mild ocean acidification[J]. Marine Biology, 2013, 160(8): 2261-2269.

Wall C B, Fan T Y, Edmunds P J. Ocean acidification has no effect on thermal bleaching in the coral Seriatopora caliendrum[J]. Coral Reefs, 2014, 33(1): 119-130.

Kurihara H. Effects of CO₂-driven ocean acidification on the early developmental stages of invertebrates[J]. Marine Ecology Progress Series, 2008, 373: 275-284.

Kurihara H, Ishimatsu A. Effects of high CO₂ seawater on the copepod (Acartia tsuensis) through all life stages and subsequent generations[J]. Marine Pollution Bulletin, 2008, 56(6): 1086-1090.

Zhang D J, Li S J, Wang G Z, et al. Impacts of CO₂-driven seawater acidification on survival, egg production rate and hatching success of four marine copepods[J]. Acta Oceanologica Sinica, 2011, 30(6): 86-94.

Zhang D J, Li S J, Wang G Z, et al. Biochemical responses of the copepod Centropages tenuiremis to CO₂-driven acidified seawater[J]. Water Science & Technology, 2012, 65(1): 30-37.

张达娟, 李少菁, 王桂忠, 等. 二氧化碳酸化对两种桡足类肌肉和卵母细胞超微结构的影响[J]. 海洋学报, 2012, 34(3): 127-133. Zhang Dajuan, Li Shaojing, Wang Guizhong, et al. Impacts of CO₂-driven acidified seawater on the muscle and oocyte ultrastructure of two marine copepods[J]. Haiyang Xuebao, 2012, 34(3): 127-133.

Wang D Z, Lin L, Chan L L, et al. Comparative studies of four protein preparation methods for proteomic study of the dinoflagellate Alexandrium sp. using two-dimensional electrophoresis[J]. Harmful Algae, 2009, 8(5): 685-691.

Redpath N T, Foulstone E J, Proud C G. Regulation of translation elongation factor-2 by insulin via a rapamycin-sensitive signalling pathway[J]. EMBO Journal, 1996, 15(9): 2291-2297.

Seibel B A, Walsh P J. Potential impacts of CO₂ injection on deep-sea biota[J]. Science, 2001, 294(5541): 319-320.

Seibel B A, Walsh P J. Biological impacts of deep-sea carbon dioxide injection inferred from indices of physiological performance[J]. The Journal of Experimental Biology, 2003, 206(4): 641-650.

Fabry V J. Ocean science: marine calcifiers in a high-CO₂ ocean[J]. Science, 2008, 320(5879): 1020-1022.

Hurley J H, Dean A M, Koshland D E, et al. Catalytic mechanism of NADP⁺-dependent isocitrate dehydrogenase: implications from the structures of magnesium-isocitrate and NADP⁺ complexes[J]. Biochemistry, 1991, 30(35): 8671-8678.

朱国萍, 黄恩启, 赵禙军. NADP-异柠檬酸脱氢酶的结构与功能[J]. 安徽师范大学学报: 自然科学版, 2007, 30(3): 366-371. Zhu Guoping, Huang Enqi, Zhao Beijun. Structure and function of NADP-Isocitrate dehydrogenase[J]. Journal of Anhui Normal University: Natural Science, 2007, 30(3): 366-371.

Hauton C, Tyrrell T, Williams J. The subtle effects of sea water acidification on the amphipod Gammarus locusta[J]. Biogeoscience, 2009, 6: 1479-1489.

Chen Z J, Kastaniotis A J, Miinalainen I J, et al. 17β-Hydroxysteroid dehydrogenase type 8 and carbonyl reductase type 4 assemble as a ketoacyl reductase of human mitochondrial FAS[J]. The FASEB Journal, 2009, 23(11): 3682-3691.

Todgham A E, Hofmann G E. Transcriptomic response of sea urchin larvae Strongylocentrotus purpuratus to CO₂-driven seawater acidification[J]. The Journal of Experimental Biology, 2009, 212(16): 2579-2594.

Tomkinson A E, Vijayakumar S, Pascal J M, et al. DNA ligases: structure, reaction mechanism, and function[J]. Chemical Reviews, 2006, 106(2): 687-699.

Bibby R, Widdicombe S, Parry H, et al. Effects of ocean acidification on the immune response of the blue mussel Mytilus edulis[J]. Aquatic Biology, 2008, 2(1): 67-74.

Burgents J E, Burnett K G, Burnett L E. Effects of Hypoxia and hypercapnic hypoxia on the localization and the elimination of Vibrio campbellii in Litopenaeus vannamei, the pacific white shrimp[J]. Biological Bulletin, 2005, 208(3): 159-168.

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