海水酸化对中华哲水蚤全蛋白表达的影响

张达娟; 郭东晖; 王桂忠; 李少菁

doi:10.3969/j.issn.0253-4193.2015.06.010

海水酸化对中华哲水蚤全蛋白表达的影响

doi: 10.3969/j.issn.0253-4193.2015.06.010

1.
天津农学院水产学院天津市水产生态及养殖重点实验室, 天津 300384
2.
厦门大学海洋与地球学院, 福建厦门 361005

基金项目: 国家自然科学基金"不同生态类型的海洋桡足类对硅藻的利用与适应的生态遗传学研究"面上项目(41276132)。

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出版历程
- 收稿日期: 2014-09-23

Proteomic study of the effects of acidified seawater on Calanus sinicus

1.
College of Fisheries, Tianjin Agricultural College, Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Tianjin 300384, China
2.
College of Ocean & Earth Science, Xiamen University, Xiamen 361005, China

摘要

摘要: 运用蛋白质组学相关技术对暴露于酸化海水中的中华哲水蚤全蛋白进行分析,结果表明,对照组、C_CO₂ 0.08%和C_CO₂ 0.20%处理组中华哲水蚤的双向电泳图谱上可以分别分辨出1 191、1 117和946个蛋白点,选取其中43个差异蛋白进行MALDI-TOF/TOF质谱鉴定,成功鉴定出23个差异蛋白,这些蛋白主要与蛋白合成和分解、能量代谢、DNA分子修复以及解毒过程有关。
- 二氧化碳 /
- 酸化 /
- 中华哲水蚤 /
- 蛋白质组学
Abstract: This study compared proteim profiles of Calanus sinicus cultured under C_CO₂ 0.08% and C_CO₂ 0.20% CO₂-acidified seawater for 4 days using a proteomic approach, and identified the differentially expressed proteins. The results are shown that, 1 191, 1 117 and 946 protein spots of C.sinicus in control, C_CO₂ 0.08% and C_CO₂ 0.20% groups were detected in the two-dimensional electrophoresis gels, respectively. The 43 protein spots were selected based on their differential expression between control and C_CO₂ 0.08% group, C_CO₂ 0.20% group, and 23 proteins of which were identified by MALDI-TOF/TOF mass spectrometry. The data demonstrated that these differentially expressed proteins were associated with protein synthesize and proteolysis, energy metabolism, DNA repaired and detoxified of organisms.
- carbon dioxide /
- ocean acidification /
- Calanus sinicus /
- proteome

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