长心卡帕藻愈伤组织的诱导与其形态建成的初步研究

李虎; 刘建国; 庞通

doi:10.3969/j.issn.0253-4193.2015.04.005

长心卡帕藻愈伤组织的诱导与其形态建成的初步研究

doi: 10.3969/j.issn.0253-4193.2015.04.005

1.
中国科学院海洋研究所, 山东青岛 266071;中国科学院大学, 北京 100049
2.
中国科学院海洋研究所, 山东青岛 266071

基金项目: 国家自然科学基金青年科学基金项目(41306154);青岛市海洋经济创新发展区域示范成果转化及产业化项目.

计量
- 文章访问数: 1693
- HTML全文浏览量: 23
- PDF下载量: 1263
- 被引次数: 0
出版历程
- 收稿日期: 2014-05-21
- 修回日期: 2014-12-18

Callus induction and morphogenesis of callus in Kappaphycus alvarezii (Rhodophyta,Solieriaceae)

1.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: 以PES液体培养基、固体培养基,采用正交设计,开展了长心卡帕藻(Kappaphycus alvarezii)棕色藻株组织培养和愈伤组织诱导实验,探索了蔗糖、光强、植物生长调节剂对新芽形成和愈伤组织形成的影响,结果表明,固体培养基、IBA、6-BA处理可诱导该藻产生愈伤组织,同时固体培养基更利于诱导形成愈伤组织;显微跟踪观察显示,该藻愈伤组织与高等植物的疏松愈伤组织不同,由细丝状细胞组成,系藻枝段中心髓部细胞脱分化形成的致密型愈伤组织;另外,愈伤枝段比新芽枝段有更高的光合和呼吸速率.
- 长心卡帕藻 /
- 组织培养 /
- 愈伤组织 /
- 氧电极 /
- 光合与呼吸
Abstract: The brown morphotype of red seaweed Kappaphycus alvarezii collected from Li'an Bay in Lingshui County,Hainan Province of China,was used as explants in order to generate new buds and induce callus. Effect of the PES (Provasoli enriched seawater) media (with or without gelling agent),light intensity,and phytoregulators (Indole-3-acetic acid (IAA),Indole-3-butyric acid (IBA),6-benzylaminopurine (6-BA)) were tested on new bud generation and callus formation by orthogonality experiment. The callus forming was observed in microscope,and the changes of the respiration rate and photosynthesis rate in explants among callus forming were detected. The callus of Kappaphycus alvarezii was achieved successfully by the combination of PES solid medium (0.8% ager),IBA,6-BA,while callus was not observed in the combination of PES liqiud medium,IBA and 6-BA. That indicates the solid medium plays an important role in callus induction. Kappaphycus alvarezii callus which consists of compact filamentous cells was different from the loose callus of superior plants. And callus derived from the medullary cells. It was found that both photosynthetic rate and respiratory rate in the branch inducing callus were higher than ones of the branch germinating new bud by means of oxygen electrode.
- Kappaphycus alvarezii /
- tissue culture /
- callus /
- oxygen electrode /
- photosynthesis and respiration

HTML全文

参考文献(36)

匡梅,曾呈奎,夏邦美.中国麒麟菜族的分类研究[J]. 海洋科学集刊,1999,41:168-236. Kuang Mei,Zeng Chengkui,Xia Bangmei. Taxonomy of Eucheumatoidae in China[J]. Studia Marina Sinica,1999,41:168-236.

Doty M S,Alvarez V B. Status,problems,advances and economics of Eucheuma farms[J]. Marine Technology Society Journal,1975,9(4): 30-36.

庞通. 卡帕藻属和麒麟菜属的种苗选育与病害防治研究. 青岛:中国科学院海洋研究所,2011. Pang Tong. Seed selection and disease control of carrageenan-producing seaweed Kappaphycus and Eucheuma. Qingdao: Institute of Oceanology,Chinese Academy of Sciences,2011.

黄园,刘建国,庞通,等. 盐度降低对长心卡帕藻原初光化学反应的影响[J]. 海洋学报,2010,32 (3): 146-152. Huang Yuan,Liu Jianguo,Pang Tong,et al. The changes of primary photochemical reactions in Kappaphycus alvarezii exposed to low salinity[J]. Haiyang Xuebao,2010,32(3): 146-152.

Liu C,Huang X,Liu J. Assessment of introduced Kappaphycus(Solieriaceae,Rhodophyta) species relationships in China with molecular markers[J]. Acta Oceanologica Sinica,2013,32(2): 59-65.

Yamada T,Ogamo A,Saito T,et al. Preparation of O-acylated low-molecular-weight carrageenans with potent anti-HIV activity and low anticoagulant effect[J]. Carbohydrate Polymers,2000,41(2): 115-120.

Yamada T,Ogamo A,Saito T,et al. Preparation and anti-HIV activity of low-molecular-weight carrageenans and their sulfated derivatives[J]. Carbohydrate Polymers,1997,32(1): 51-55.

Haijin M,Xiaolu J,Huashi G. A κ-carrageenan derived oligosaccharide prepared by enzymatic degradation containing anti-tumor activity[J]. Journal of applied phycology,2003,15(4): 297-303.

Hurtado A Q,Biter A B. Plantlet regeneration of Kappaphycus alvarezii var. adik-adik by tissue culture[J]. Journal of Applied Phycology,2007,19(6): 783-786.

Yunque D A T,Tibubos K R,Hurtado A Q,et al. Optimization of culture conditions for tissue culture production of young plantlets of carrageenophyte Kappaphycus[J]. Journal of Applied Phycology,2011,23(3): 433-438.

刘建国,庞通,王莉,等. 导致热带产卡拉胶海藻大规模死亡原因分析与藻株抗病差异性比较[J]. 海洋与湖沼,2009,40(2): 235-241. Liu Jianguo,Pang Tong,Wang Li,et al. The reasons causing catastrophic death in tropical carrageenan producing seaweeds and their difference in resistance to illness[J]. Oceanologia et Limnologia Sinica,2009,40(2): 235-241.

Aguirre-Lipperheide M,Estrada-Rodríyuez F J,Evans L V. Facts,problems,and needs in seaweed tissue culture: an appraisal[J]. Journal of Phycology,1995,31(5): 677-688.

Polne-Fuller M,Gibor A. Calluses and callus-like growth in seaweeds: induction and culture. Twelfth International Seaweed Symposium. Springer Netherlands,1987: 131-138.

Kumar G R,Reddy C R K,Jha B. Callus induction and thallus regeneration from callus of phycocolloid yielding seaweeds from the Indian coast[J]. Journal of Applied Phycology,2007,19(1): 15-25.

Kawashima Y,Tokuda H. Callus formation in Ecklonia cava Kjellman (Laminariales,Phaeophyta). Thirteenth International Seaweed Symposium. Springer Netherlands,1990: 375-380.

Kawashima Y,Tokuda H. Regeneration from callus of Undaria pinnatifida(Harvey) Suringar (Laminariales,Phaeophyta). Fourteenth International Seaweed Symposium. Springer Netherlands,1993: 385-389.

Bouzon Z L,Schmidt E C,Almeida A C,et al. Cytochemical characterization and ultrastructural organization in calluses of the agarophyte Gracilariopsis tenuifrons(Gracilariales,Rhodophyta)[J]. Micron,2011,42(1): 80-86.

Collantes G,Melo C,Candia A. Micropropagation by explants of Gracilaria chilensis Bird,McLachlan and Oliveira[J]. Journal of applied phycology,2004,16(3): 203-213.

Yokoya N S. Apical callus formation and plant regeneration controlled by plant growth regulators on axenic culture of the red alga Gracilariopsis tenuifrons(Gracilariales,Rhodophyta)[J]. Phycological Research,2000,48(3): 133-142.

Yokoya N S,West J A,Luchi A E. Effects of plant growth regulators on callus formation,growth and regeneration in axenic tissue cultures of Gracilaria tenuistipitata and Gracilaria perplexa(Gracilariales,Rhodophyta)[J]. Phycological Research,2004,52(3): 244-254.

Kaczyna F,Megnet R. The effects of glycerol and plant growth regulators on Gracilaria verrucosa (Gigartinales,Rhodophyceae)[J]. Hydrobiologia,1993,268(1): 57-64.

Dawes C J,Koch E W. Branch,micropropagule and tissue culture of the red algae Eucheuma denticulatum and Kappaphycus alvarezii farmed in the Philippines[J]. Journal of applied phycology,1991,3(3): 247-257.

Hayashi L,Yokoya N S,Kikuchi D M,et al. Callus induction and micropropagation improved by colchicine and phytoregulators in Kappaphycus alvarezii (Rhodophyta,Solieriaceae)[J]. Journal of Applied Phycology,2008,20(5): 653-659.

Provasoli L. Media and prospects for the cultivation of marine algae. Cultures and Collections of Algae. Proceedings of the US-Japan Conference,Hakone,September 1966. Japanese Society for Plant Physiology,1968: 63-75.

卓书辉,郭彦岑,尹群健,等. 长心卡帕藻外植体培养及不定芽诱导[J]. 广东农业科学,2012,39(23): 126-128. Zhuo Shuhui,Guo Yancen,Yin Qunjian,et al. Explant regeneration and shoot induction of Kappaphycus alvarezii[J].Guangdong Agricultural Sciences,2012,39(23): 126-128.

Lee T M,Chang Y C,Lin Y H. Differences in physiological responses between winter and summer Gracilaria tenuistipitata(Gigartinales,Rhodophyta) to varying temperature[J]. Botanical Bulletin of Academia Sinica,1999,40:93-100.

张栩,李大鹏,施定基,等. 裙带菜配子体和幼孢子体的光合作用特性[J]. 海洋科学,2004,28(11): 20-27. Zhang Xu,Li Dapeng,Shi Dingji,et al. Photosynthesis properties of gametophytes and juvenile sporophytes of algae,Undaria pinnatifida[J]. Marine Sciences,2004,28(11): 20-27.

Reddy C R K,Jha B,Fujita Y,et al. Seaweed micropropagation techniques and their potentials: an overview[J]. Journal of Applied Phycology,2008,20(5): 609-617.

Yokoya N S,Yoneshigue-Valentin Y. Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta[J]. Revista Brasileira de Farmacognosia,2011,21(2): 334-339.

Bindu M S,Levine I A. The commercial red seaweed Kappaphycus alvarezii——an overview on farming and environment[J]. Journal of Applied Phycology,2011,23(4): 789-796.

Gusev M V,Tambiev A H,Kirikova N N,et al. Callus formation in seven species of agarophyte marine algae[J]. Marine Biology,1987,95(4): 593-597.

Yokoya N S,vila M,Piel M I,et al. Effects of plant growth regulators on growth and morphogenesis in tissue culture of Chondracanthus chamissoi (Gigartinales,Rhodophyta)[J]. Journal of Applied Phycology,2014,26(2): 819-823.

Yokoya N S,Handro W. Effects of plant growth regulators and culture medium on morphogenesis of Solieria filiformis(Rhodophyta) cultured in vitro[J]. Journal of applied phycology,2002,14(2): 97-102.

Robledo D R,Garcia-Reina G. Apical callus formation in Solieria filiformis(Gigartinales,Rhodophyta) cultured in tanks[J]. Hydrobiologia,1993,260(1): 401-406.

Zitta C S,Rover T,Hayashi L,et al. Callus ontogeny of the Kappaphycus alvarezii (Rhodophyta,Gigartinales) brown tetrasporophyte strain[J]. Journal of Applied Phycology,2013: 1-15.

Reddy C R K,Kumar G,Siddhanta A K,et al. In vitro somatic embryogenesis and regeneration of somatic embryos from pigmented callus of kappaphycus alvarezii(doty) doty (rhodophyta,gigartinales) [J]. Journal of phycology,2003,39(3): 610-616.

施引文献

资源附件(0)

访问统计