长心卡帕藻愈伤组织的诱导与其形态建成的初步研究

李虎; 刘建国; 庞通

doi:10.3969/j.issn.0253-4193.2015.04.005

长心卡帕藻愈伤组织的诱导与其形态建成的初步研究

doi: 10.3969/j.issn.0253-4193.2015.04.005

1.
中国科学院海洋研究所, 山东青岛 266071;中国科学院大学, 北京 100049
2.
中国科学院海洋研究所, 山东青岛 266071

基金项目: 国家自然科学基金青年科学基金项目(41306154);青岛市海洋经济创新发展区域示范成果转化及产业化项目.

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出版历程
- 收稿日期: 2014-05-21
- 修回日期: 2014-12-18

Callus induction and morphogenesis of callus in Kappaphycus alvarezii (Rhodophyta,Solieriaceae)

1.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

摘要

摘要: 以PES液体培养基、固体培养基,采用正交设计,开展了长心卡帕藻(Kappaphycus alvarezii)棕色藻株组织培养和愈伤组织诱导实验,探索了蔗糖、光强、植物生长调节剂对新芽形成和愈伤组织形成的影响,结果表明,固体培养基、IBA、6-BA处理可诱导该藻产生愈伤组织,同时固体培养基更利于诱导形成愈伤组织;显微跟踪观察显示,该藻愈伤组织与高等植物的疏松愈伤组织不同,由细丝状细胞组成,系藻枝段中心髓部细胞脱分化形成的致密型愈伤组织;另外,愈伤枝段比新芽枝段有更高的光合和呼吸速率.
- 长心卡帕藻 /
- 组织培养 /
- 愈伤组织 /
- 氧电极 /
- 光合与呼吸
Abstract: The brown morphotype of red seaweed Kappaphycus alvarezii collected from Li'an Bay in Lingshui County,Hainan Province of China,was used as explants in order to generate new buds and induce callus. Effect of the PES (Provasoli enriched seawater) media (with or without gelling agent),light intensity,and phytoregulators (Indole-3-acetic acid (IAA),Indole-3-butyric acid (IBA),6-benzylaminopurine (6-BA)) were tested on new bud generation and callus formation by orthogonality experiment. The callus forming was observed in microscope,and the changes of the respiration rate and photosynthesis rate in explants among callus forming were detected. The callus of Kappaphycus alvarezii was achieved successfully by the combination of PES solid medium (0.8% ager),IBA,6-BA,while callus was not observed in the combination of PES liqiud medium,IBA and 6-BA. That indicates the solid medium plays an important role in callus induction. Kappaphycus alvarezii callus which consists of compact filamentous cells was different from the loose callus of superior plants. And callus derived from the medullary cells. It was found that both photosynthetic rate and respiratory rate in the branch inducing callus were higher than ones of the branch germinating new bud by means of oxygen electrode.
- Kappaphycus alvarezii /
- tissue culture /
- callus /
- oxygen electrode /
- photosynthesis and respiration

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参考文献(36)

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