Effect on lipid accumulation of marine oil-rich microalgae under different temperature and CO2 enrichment cultivation
-
摘要: 本实验分别针对3株低温藻株:微拟球藻Nannochloropsis sp. ZL-12、四爿藻Tetraselmis chui ZL-33和小球藻Chlorella sp. ZL-45,3株中温藻株:球等鞭金藻Isochrysis galbana CCMM5001、等鞭金藻Isochrysis sp. CCMM5002和微拟球藻Nannochloropsis sp. CCMM7001,3株高温藻株:微拟球藻Nannochloropsis sp. JN1、绿色巴夫藻Pavlova viridis JN2和海洋小球藻Chlorella sp. JN3,研究了在通入0.03%(空气)、5%、10% 3个CO2浓度梯度条件下的生长特性,同时考察了其总酯及中性脂的累积情况。结果显示,富碳培养有利于不同温度条件下9株藻株的生长,除微拟球藻Nannochloropsis sp. CCMM7001最适生长的CO2浓度为5%外,其余8株藻株最适生长的CO2浓度均为10%。在低温和高温条件下,6株海洋富油微藻在通入10% CO2时具有最大生物量产率,在中温条件下球等鞭金藻和等鞭金藻在通入10% CO2时获得最大生物量产率,而微拟球藻在通入5%时获得最大生物量产率,随着CO2浓度的增加,9株藻株的总脂含量和中性脂含量有明显提高。低温和中温藻株的总脂含量高于高温藻株的总脂含量,从中性脂的累积规律来看,9株藻株均在平台期的累积达到最大值,GC-MS分析结果表明,9株微藻适合制备生物柴油的C14~C18系脂肪酸相对含量在不同CO2条件下基本保持不变,维持在90%左右。实验结果显示,所研究的藻株作为富油高固碳优良藻株,具备用于海洋生物质能耦合CO2减排开发的潜力。Abstract: Nine marine microalgae were cultured under different CO2 concentrations of ambient air (0.03%),5% and 10%,respectively. Nine marine microalgae include 3 strains of cold resisting marine microalgae (Nannochloropsis sp. ZL-12,Tetraselmis chui ZL-33,Chlorella sp. ZL-45),3 strains of mesophilic marine microalgae (Isochrysis galbana CCMM5001,Isochrysis sp. CCMM5002 and Nannochloropsis sp. CCMM7001) and 3 strains of heat resisting marine microalgae (Nannochloropsis sp. JN1,Pavlova viridis JN2 and Chlorella sp. JN3). The growth characterization,accumulation of total and neutral lipid of these microalgae were investigated. The results showed that CO2 enrichment cultivation could increase the growth of all nine microalgae,but the optimum CO2 concentrations were different. The optimum CO2 concentration of Nannochloropsis sp. CCMM7001 was 5%,The optimum CO2 concentration of 8 strains of marine microalgae was 10%. 3 strains of cold resisting marine microalgae and 3 strains of heat resisting marine microalgae reached the maximum biomass yield when culturing with 10% CO2. 2 strains of mesophilic marine microalgae (Isochrysis galbana CCMM5001 and Isochrysis sp. CCMM5002) run up to the maximum biomass yield when culturing with 10% CO2. However,the maximum biomass yield of Nannochloropsis sp. CCMM7001 was (122.25±1.17) mg/(L·d) when culturing with 5% CO2. With the increased CO2 concentration,the total lipid and neutral lipid of three microalgae improved significantly. The total lipid content of 3 strains of cold resisting marine microalgae and 3 strains of mesophilic marine microalgae was higher than 3 heat resisting marine microalgae. The maximum neutral lipid content of 9 strains of microalgae could be accumulated in stationary phase. The fatty acid analysis of 9 strains of microalgae showed the relative content of C14-C18 fatty acid which suitable for biodiesel preparation maintained at 90% when culturing with different CO2 concentration. The results indicate our marine oleaginous microalgae with high carbon dioxide fixation ability are the potential excellent strains for marine bioenergy development coupled with CO2 emission reduction.
-
Boden T A,Marland G,Andres R J. Global,Regional,and National Fossil-Fuel CO2 Emissions[R]. Carbon Dioxide Information Analysis Center,Oak Ridge National Laboratory,U. S. Department of Energy,Oak Ridge,Tenn. 2012,U. S. A. doi 10. 3334/CDIAC/00001_V2013 杨忠华,杨改,李方芳,等. 利用微藻固定CO2实现碳减排的研究进展[J]. 生物加工过程,2011,9(1): 66-76. Salih F M. Microalgae tolerance to high concentrrations of carbon dioxide: a review[J]. Journal of Environmental Protection,2011,2(5): 648-654. Lee Y K,Tay H S. High CO2 partial pressure depresses productivity and bioenergetic growth yield of Chlorella pyrenoidosa culture[J]. Journal of Applied Phycology,1991,3(2): 95-101. 白冰,李小春,刘延锋,等. 中国CO2集中排放源调查及其分布特征[J]. 岩石力学与工程学报,2006,25(1): 2918-2924. Guillard R R L,Ryther J H. Studies of marine planktonic diatoms: I. Cyclotella nana Hustedt,and Detonula confervacea (cleve) Gran[J]. Canadian Journal of Microbiology,1962,8(2): 229-239. Huerlimann R,de Nys R,Heimann K. Growth,lipid content,productivity,and fatty acid composition of tropical microalgae for scale-up production[J]. Biotechnology and Bioengineering,2010,107(2): 245-257. Tang D H,Han W,Li P L,et al. CO2 biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO2 levels[J]. Bioresource Technology,2011,102(3): 3071-3077. Chen W,Sommerfeld M,Hu Q. Microwave-assisted nile red method for in vivo quantification of neutral lipids in microalgae[J]. Bioresource Technology,2011,102(1): 135-141. Chen W,Zhang C W,Song L R,et al. A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae[J]. Journal of Microbiological Methods,2009,77(1): 41-47. 王金娜,严小军,周成旭,等. 产油微藻的筛选及中性脂动态积累过程的检测[J]. 生物物理学报,2010,26(6): 472-480. Bligh E G,Dyer W J. A rapid method of total lipid extraction and purification[J]. Canadian Journal of Biochemistry and Physiology,1959,37(8): 911-917. Sánchez Pérez J A,Rodríguez Porcel E M,Casas López J L,et al. Shear rate in stirred tank and bubble column bioreactors[J]. Chemical Engineering Journal,2006,124(1/3): 1-5. Kirk J T O. Light and Photosynthesis in Aquatic Ecosystems[M]. Cambridge: Cambridge University Press,1983: 101-105. Hu H H,Gao K S. Optimization of growth and fatty acid composition of a unicellular marine picoplankton,Nannochloropsis sp.,with enriched carbon sources[J]. Biotechnology Letters,2003,25(5): 421-425. Chrismadha T,Borowitzka M A. Effect of cell density and irradiance on growth,proximate composition and eicosapentaenoic acid production ofphaeodactylum tricornutum grown in a tubular photobioreactor[J]. Journal of Applied Phycology,1994,6(1): 67-74. Li S Y,Shabtai Y,Arad S. Production and composition of the sulphated cell wall polysaccharide of Porphyridium(Rhodophyta) as affected by CO2 concentration[J]. Phycologia,2000,39(4): 332-336. Yue L H,Chen W G. Isolation and determination of cultural characteristics of a new highly CO2 tolerant fresh water microalgae[J]. Energy Conversion and Management,2005,46(11/12): 1868-1876. Yoon J H,Sim S J,Kim M S,et al. High cell density culture of Anabaena variabilis using repeated injections of carbon dioxide for the production of hydrogen[J]. Inter Hydrogen Energy,2002,27(11/12): 1265-1270. 欧阳峥嵘,温小斌,耿亚红,等. 光照强度、温度、pH、盐度对小球藻(Chlorella)光合作用的影响[J]. 武汉植物学研究,2010,28(1): 49-55. 徐宁,吕颂辉,陈菊芳,等. 温度和盐度对锥状斯氏藻生长的影响[J]. 海洋环境科学,2004,23(3): 36-39. 高春燕,程丽华,张林,等. 小球藻光生物反应器脱除空气中二氧化碳的研究[J]. 膜科学与技术,2005,25(4): 8-12. Kimura K,Yamaoka M,Kamisaka Y. Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence[J]. J Microbiol Meth,2004,56(3): 331-338. Elsey D,Jameson D,Raleigh B,et al. Fluorescent measurement of microalgal neutral lipids[J]. J Microbiol Meth,2007,68(3): 639-642. Ben-Amotz A,Tomdene T C,Thoms W H. Chemical profile of selected species of Microalgae with emphasis on lipids[J]. J Phycol,1985,21(1): 72-81. Liang Y,Mai K S,Sun S C. Total lipid and fatty acid composition of seven Chaetoceros strains[J]. Trans Oceanol Limnol,2000(3): 29-33. 石娟,潘克厚. 不同培养条件对微藻总脂含量和脂肪酸组成的影响[J]. 海洋水产研究,2004,25(6): 79-86. 徐进,徐旭东,方仙桃,等. 高产油小球藻的筛选及其油脂分析[J]. 水生生物学报,2012,36(3): 426-433. 朱顺妮,王忠铭,尚常花,等. 微藻脂肪合成与代谢调控[J]. 化学进展,2011,23(10): 2169-2177. 魏东,张学成,邹立红,等. 细胞生长时期对两种海洋微藻总脂含量和脂肪酸组成的影响[J]. 青岛海洋大学学报(自然科学版),2000,30(3): 503-509. Vargas M A,Rodríguez H,Moreno J,et al. Biochemical composition and fatty acid content of filamentous nitrogen-fixing cyanobacteria[J]. Journal of Phycology,1998,34(5): 812-817. Tsuzuki M,Ohnuma E,Sato N,et al. Effects of CO2 concentration during growth on fatty acid composition in microalgae[J]. Plant Physiology,1990,93(3): 851-857.
点击查看大图
计量
- 文章访问数: 1698
- HTML全文浏览量: 10
- PDF下载量: 1011
- 被引次数: 0