北极海洋沉积物中可培养细菌及其多样性分析

王桢; 李阳; 车帅; 林学政

doi:10.3969/j.issn.0253-4193.2014.10.012

北极海洋沉积物中可培养细菌及其多样性分析

doi: 10.3969/j.issn.0253-4193.2014.10.012

国家海洋局第一海洋研究所国家海洋局海洋生物活性物质重点实验室, 山东青岛 266061

基金项目: 南北极环境综合考察与评估专项（CHINARE2012-03-05，CHINARE2013-03-05）。

计量
- 文章访问数: 1716
- HTML全文浏览量: 9
- PDF下载量: 1474
- 被引次数: 0
出版历程
- 收稿日期: 2014-03-16
- 修回日期: 2014-04-08

Diversity analysis of culturable bacteria isolated from marine sediments of Arctic

Key Lab of Marine Bioactive Substances State Oceanic Administration, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

摘要

摘要: 利用Zobell 2216E培养基和涂布平板法对北极海洋沉积物中可培养细菌进行分离纯化，并利用16S rRNA基因进行分子鉴定与系统发育分析。根据菌落形态学特征，从59个站点的沉积物样品中共分离纯化获得570株细菌；基于16S rRNA基因的分子鉴定与系统发育分析表明，分离到的可培养细菌分别属于细菌域的4个门，5个纲，12个目，23个科，47个属，102个种，其中γ-Proteobactria占绝大多数；有14株菌株与模式菌株的16S rRNA基因序列相似性小于97%，为6个潜在的新种。北极海域的海洋沉积物中存在着丰富的微生物种质资源，为开发新型生物活性物质和特殊功能基因打下了基础。
- 北极 /
- 海洋沉积物 /
- 细菌多样性 /
- 系统发育分析
Abstract: Culturable bacteria were isolated by use of marine Zobell 2216E medium and spread plate method. Molecular identification and phylogenetic analysis were carried out on the basis of 16S rRNA gene. According to the distinct morphological character of bacterial colony on the marine Zobell 2216E medium plate,a total of 570 isolates were obtained from Arctic sediments. Molecular identification and phylogenetic analysis showed that these isolates belonged to four phylum of the bacteria domain,5 classes,47 genera,and 102 species in total. 14 isolates had 16S rRNA gene similarity less than 97% with the closest type strain were grouped into 6 clusters. It indicated that these isolates might be potential novel species. A high diversity of culturable bacteria were isolated from marine sediment of Arctic in term of both species number and phylogenetic composition,which deserves further study and exploitation,such as novel bioactive substances and functional genes.
- Arctic /
- marine sediment /
- bacterial diversity /
- phylogenetic analysis

HTML全文

参考文献(20)

林学政, 边际, 何培青. 极地微生物低温适应性的分子机制[J]. 极地研究, 2003, 15(1): 75-82.

Zeng Y X, Zou Y, Chen B, et al. Phylogenetic diversity of sediment bacteria in the northern Bering Sea[J]. Polar Biology, 2011, 34(6): 907-919.

Bano N, Hollibaugh J T. Phylogenetic composition of bacterioplankton assemblages from the Arctic Ocean[J]. Applied and Environmental Microbiology, 2002, 68(2): 505-518.

Skidmore M L, Fought J M, Sharp M J. Microbial life beneath a high Arctic glacier[J]. Applied and Environmental Microbiology, 2000, 66(8): 3214-3220.

Sahm K, Knoblauch C, Amann R. Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments[J]. Applied and Environmental Microbiology, 1999, 65(9): 3976-3981.

Knoblauch C, Jorgensen B B, Harder J. Community size and metabolic rates of psychrophilic sulfate-reducing bacteria in Arctic marine sediments[J]. Applied and Environmental Microbiology, 1999, 65(9): 4230-4233.

Junge K, Imhoff F, Staley T, et al. Phylogenetic diversity of numerically important Arctic sea-ice bacteria cultured at subzero temperature[J]. Microbial Ecology, 2002, 43(3): 315-328.

林学政, 陈靠山, 何培青, 等.种植盐地碱蓬改良滨海盐渍土对土壤微生物区系的影响[J].生态学报, 2006, 26(3):801-807.

Kim O S, Cho Y J, Lee K, et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species[J]. International Journal of Systematic and Evolutionary Microbiology, 2012, 62(Pt 3): 716-721.

李贺, 林学政, 何培青, 等. 南极抗细菌活性菌株的筛选及系统发育分析[J]. 微生物学通报, 2011, 38(2): 264-269.

林曦, 杨祥胜, 曾润颖. 北极深海沉积物中嗜冷细菌的系统发育分析[J]. 厦门大学学报(自然科学版), 2007, 46(3): 411-417.

Srinivas T N R, Nageswara Rao S S S, Vishnu V R P, et al. Bacterial diversity and Bioprospecting for cold-active lipases, amylases and proteases, from Culturable bacteria of Kongsfjorden and Ny-Ålesund, Svalbard, Arctic[J]. Current Microbiology, 2009, 59(5): 537-547.

Groudieva T, Kambourova M, Yusef H, et al. Diversity and cold-active hydrolytic enzymes of culturable bacteria associated with Arctic sea ice, Spitzbergen[J]. Extremophiles, 2004, 8(6): 475-488.

Holt J G, Krieg N R, Sneath P H A, et al. Bergey's Manual of Determinative Bacteriology (9th Edition)[M]. USA: The Williams & Wilkins Company, 1994: 335-346.

Ma YF, Wang L, Shao Z Z. Pseudomonas, the dominant poly-cyclic aromatic hydrocarbon-degrading bacteria isolated from Antarctic soils and the role of large plasmids in horizontal gene transfer[J]. Environmental Microbiology, 2006, 8(3): 455-465.

Cui Z S, Lai Q L, Dong C M, et al. Biodiversity of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria from Deep Sea Sediments of the Middle Atlantic Ridge[J]. Environmental Microbiology, 2008, 10(8): 2138-2149.

Kato C, Nogi Y. Correlation between phylogenetic structure and function: examples from deep-sea Shewanella[J]. FEMS Microbiology Ecology, 2001, 35(3): 223-230.

孙风芹, 汪保江, 李光玉, 等. 南海南沙海域沉积物中可培养微生物及其多样性分析[J]. 微生物学报, 2008, 48(12): 1578-1587.

Sait M, Hugenholtz P, Janssen P H. Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation independent studies[J]. Environmental Microbiology, 2002, 4(11): 654-666.

王鹏, 王风平. 南极阿德雷岛淡水湖沉积物细菌群落研究[J]. 极地研究, 2009, 21(2): 100-107.

施引文献

资源附件(0)

访问统计