24-表油菜素内酯对龙须菜抗高温胁迫的研究

李静; 王俏俏; 徐年军; 孙雪; 范美华

doi:10.3969/j.issn.0253-4193.2014.08.009

24-表油菜素内酯对龙须菜抗高温胁迫的研究

doi: 10.3969/j.issn.0253-4193.2014.08.009

宁波大学应用海洋生物技术教育部重点实验室, 浙江宁波 315211;宁波大学海洋学院海洋生物工程重点实验室, 浙江宁波 315211

基金项目: 国家自然科学基金项目（31072229，41376151）；浙江省钱江人才项目（2007R10038）。

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出版历程
- 收稿日期: 2013-10-18
- 修回日期: 2013-12-26

Effects of 24-epibrassinolide on the resistance of Gracilaria lemaneiformis to high temperature

Key Laboratory of Applied Marine Biotechnology of the Ministry of Education, Ningbo University, Ningbo 315211, China;Key Laboratory of Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo 315211, China

摘要

摘要: 本研究采用不同浓度24-表油菜素内酯处理海洋红藻龙须菜，研究热激胁迫后龙须菜的生长速率、叶绿素荧光参数、抗氧化酶活性、甘露醇、丙二醛、藻胆蛋白含量，以及 HSP70、PE、GR 基因的相对表达水平变化规律。结果显示，0.1 mg/L 24-表油菜素内酯的处理组生长速率最大，显著高于其他处理组及对照组；叶绿素荧光参数Fv/Fm、qP和ΦPS Ⅱ均随高温逆境时间延长呈现下降趋势，荧光参数NPQ随着高温逆境时间延长呈现先上升后下降的趋势，且处理组各项荧光参数值均高于对照组；处理组的抗氧化酶（SOD和POD）的酶活显著高于对照组；甘露醇及藻胆蛋白含量均在0.1 mg/L处理组最高，在第三天达到最大值，而丙二醛含量则在0.1 mg/L处理组最低，在第三天达到最小值；处理组 HSP70、PE、GR 基因的相对表达量均高于对照组，且0.1 mg/L处理组各基因表达量最高。以上结果表明24-表油菜素内酯能提高龙须菜的抗高温能力，且0.1 mg/L处理组效果最好。
- 龙须菜 /
- 24-表油菜素内酯 /
- 热激胁迫 /
- 抗高温
Abstract: To explore the effect of 24-epibrassinolide on the marine red algae Gracilaria lemaneiformis under high temperature stress,the growth rate,chlorophyll fluorescence parameters,antioxidant enzyme activity,as well as the contains of mannitol,malondialdehyde,phycobiliprotein,and the relative expression levels of HSP70,PE and GR were detected in G. lemaneiformis after treated with different concentration of 24-epibrassinolide and heat shock stress. Results showed that the growth rate of 0.1 mg/L treatment group was significantly higher than that of other groups. Chlorophyll fluorescence parameters of Fv/Fm,qP and ΦPS Ⅱ decreased while parameters of NPQ first increased and then decreased along with the extension of high temperature stress. Chlorophyll fluorescence parameters in all treatment groups were higher than those in the control groups. The activities of SOD and POD in treatment groups were significantly higher than those in the control groups. The detected mannitol and phycobiliprotein levels were the highest in 0.1 mg/L treatment group,and increased to the summit at 3 d post treatment. The detected malondialdehyde level was the lowest in 0.1 mg/L treatment group,and decreased to the bottom at 3 d post treatment. The relative gene expression levels of HSP70,PE and GR in treatment groups were higher than those in the control groups. They all presented the highest in 0.1 mg/L treatment group. The results indicated that 24-epibrassinolide could improve high temperature resistance of G. lemaneiformis,and the 0.1 mg/L treatment group showed the best effect.
- Gracilaria lemaneiformis /
- 24-epibrassinolide /
- high temperature stress /
- high temperature resistance

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