3种海洋硅藻不同培养阶段挥发性成分的比较分析

陈姣; 徐继林; 李艳; 周成旭; 严小军

doi:10.3969/j.issn.0253-4193.2014.08.006

3种海洋硅藻不同培养阶段挥发性成分的比较分析

doi: 10.3969/j.issn.0253-4193.2014.08.006

宁波大学应用海洋生物技术教育部重点实验室, 浙江宁波 315211

基金项目: 国家自然科学基金（31172448）；浙江省公益性项目（2014C32081）；宁波市科技攻关项目（2013C10014）；宁波市海洋藻类生物技术创新团队项目（2011B81007）。

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出版历程
- 收稿日期: 2013-10-21
- 修回日期: 2014-04-15

Analysis of volatile components from three marine diatoms at different growth stages

Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China

摘要

摘要: 本文应用顶空固相微萃取气相色谱-质谱联用技术，对威氏海链藻Thalassiosira weissflogii、小新月菱形藻Nitzschia closterium、角毛藻Chaetoceros calcitrons 3种硅藻的挥发性成分进行分析。结果表明，在3种硅藻的培养过程中，其挥发性成分除了壬醛及醇类呈现先增后降的趋势外，大多数不饱和醛类的含量在不断增加，烷烯烃类物质在不断减少，而乙酸乙酯、2-甲基-2-丙酸甲酯这类酯类化合物在角毛藻衰败期会显著富集。通过对代表性饵料硅藻的挥发性成分进行研究，可为海水养殖过程中微藻投喂种类、投喂时机的选择提供理论依据，并为海洋硅藻的进一步开发奠定基础。
- 海洋硅藻 /
- 挥发性成分 /
- 培养阶段 /
- 固相微萃取-气相色谱-质谱联用
Abstract: The volatile components in marine diatoms Thalassiosira weissflogii,Nitzschia closterium and Chaetoceros calcitrons were analyzed by head space solid-phase micro-extraction and gas chromatography-mass spectrometry. The differences of volatile components among three diatoms at different growth stages were studied by SIMCA-P software. The results showed that the content of most unsaturated aldehydes increased through the entire culture stages of three diatoms except for a saturated aldehyde,Nonanal,whose content first increased and then decreased.The content of Alkanes continued to decline. Alcohols also increased first and then decreased. Esters including acetic acid ethyl ester and 2-methyl-2-Propenoic acid methyl ester were significantly enriched in the decline phase of C. calcitrons. According to the present study on volatile components of these diatoms,we can provide important reference not only for choosing microalgae species and feeding period in marine aquaculture,but also for studying the reason of odor formation and further development and utilization of marine diatom.
- marine diatom /
- volatile components /
- growth phase /
- head space solid-phase micro-extraction and gas chromatography-mass spectrometry

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