Acute hypoxia stress on blood biochemical indexes in selective group of Trachinotus ovatus
-
摘要: 本文研究了水环境急性低氧胁迫对卵形鲳鲹Trachinotus ovatus选育群体血液生化指标的影响。实验设计了急性低氧试验组和对照组,每组3个平行。结果显示,急性低氧胁迫后选育群体的血清离子含量与对照组相比都有不同程度的升高,其中钠、氯、钙浓度与对照组相比差异显著(p<0.05),铁浓度极显著高于对照组(p<0.01);血清蛋白、尿酸、肌酐、血脂、血糖等指标的差异较大,其中尿酸、肌酐极显著高于对照组(p<0.01),总蛋白、总胆固醇显著低于对照组(p<0.05);血清中肌酸激酶含量极显著高于对照组(p<0.01),肌酸激酶同工酶显著高于对照组(p<0.05),γ-谷氨酰转肽酶含量显著低于对照组(p<0.05);其他指标均有不同程度的升高或降低,但差异不显著。研究表明,急性低氧胁迫造成卵形鲳鲹鳃、肾、心脏和肝脏损伤,免疫力下降。Abstract: This study dealt with the influence of acute hypoxia stress on blood biochemical indices in selective group of Trachinotus ovatus. Two experiment groups were established in terms of acute hypoxia stress group and control in triplicate for each. The results showed that after acute hypoxia stress ionic components in serum in selective group increased to varying degrees comparing with the control group. Sodium, chloride and calcium concentrations were significantly different (p<0.05) from the control group, and level of iron was greatly higher than that in control group(p<0.01). There were significant differences between two groups in terms of serum protein, uric acid, creatinine, blood fat and blood glucose, with extreme significant higher in creatinine and uric acid contents(p<0.01), and significant lower in total protein and total cholesterol than that in control(p<0.05). Extreme significant higher in serum creatine kinase(p<0.01), significant higher in creatine kinase isoenzyme(p<0.05), and significant lower in γ-glutamyltranspeptidase were found than that in control (p<0.05). Other indexes increased or decreased to varying degrees with no significantly difference. Above research indicated that acute hypoxia stress resulted in damage in gill, kidney, heart and liver, as well as weak immunity in fish.
-
Key words:
- Trachinotus ovatus /
- selective group /
- acute hypoxia stress /
- blood /
- biochemical index
-
区又君,李加儿.卵形鲳鲹的早期胚胎发育[J]. 中国水产科学,2005,12(6):786-789. 区又君,何永亮,李加儿.卵形鲳鲹胚后发育阶段鳃的分化和发育[J]. 中国水产科学,2012,19(1):13-21. 蔡文超,区又君,李加儿,等.卵形鲳鲹免疫器官的早期发育[J]. 南方水产科学,2012,8(5):39-45. 吉磊,区又君,李加儿.卵形鲳鲹3个养殖群体的微卫星多态性分析[J]. 热带海洋学报,2011,30(3):62-68. 杜强,林黑着,牛津,等.卵形鲳鲹幼鱼的赖氨酸需求量[J]. 动物营养学报,2011,23 (10):1725-1732. 黄忠,林黑着,牛津,等.肌醇对卵形鲳鲹生长、饲料利用和血液指标的影响[J]. 南方水产科学,2011,7(3):39-44. Xu Haidong,Feng Juan,Guo Zhixun,et al. Detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification[J]. J of Virological Methods,2010,163(1):123-128. 许海东,区又君,郭志勋,等.神经坏死病毒对卵形鲳鲹的致病性及外壳蛋白基因序列分析[J]. 上海海洋大学学报,2010,19(4):482-488. 苏慧,区又君,李加儿,等.饥饿对卵形鲳鲹幼鱼不同组织抗氧化能力、Na+/K+-ATP酶活力和鱼体生化组成的影响[J]. 南方水产科学,2012,8(6):28-36. 区又君,罗奇,李加儿,等.卵形鲳鲹消化酶活性的研究:Ⅰ.成鱼和幼鱼消化酶活性在不同消化器官中的分布及其比较[J]. 南方水产科学,2011,7(1):50-55. 区又君,罗奇,李加儿. 卵形鲳鲹碱性磷酸酶和酸性磷酸酶的分布及其低温保存[J].南方水产科学,2011,7(2):49-54. Pero T,Nika G',Valter K,et al. Preliminary information on feeding and growth of pompano,Trachinotus ovatus(Linnaeus 1758) (Pisces; Carangidae) in captivity[J]. Aquaculture International,2004,12(4/5): 387-393. Sparks J S,Dunlap P V,Smith W L. Evolution and diversification of a sexually dimorphic luminescent system in ponyfishes (Teleostei: Leiognathidae),including diagnoses for two new genera[J]. Cladistics,2005,21(4):305-327. Chen W L,Bonillo C,Lecointre G. Repeatability of clades as a criterion of reliability: a case study for molecular phylogeny of Acanthomorpha (Teleostei) with larger number of taxa[J]. Molecular Phylogenetics and Evolution,2003,26:262-288. 王刚,李加儿,区又君,等.卵形鲳鲹胚胎及早期仔鱼耗氧量的研究[J].生态科学,2010,29(6):518-523. 王刚,李加儿,区又君,等.卵形鲳鲹幼鱼耗氧率和排氨率的初步研究[J].动物学杂志,2010,45(3):116-121. 王刚,李加儿,区又君,等.环境因子对卵形鲳鲹幼鱼耗氧率和排氨率的影响[J]. 动物学杂志,2011,46(6):80-87. 王刚,李加儿,区又君,等.温度、盐度、pH对卵形鲳鲹幼鱼离体鳃组织耗氧量的影响[J]. 南方水产科学,2011,7(5):37-42. 许晓娟,李加儿,区又君,等.深水网箱养殖卵形鲳鲹血液指标[J]. 动物学杂志,2008,43(6): 109-116. 范春燕,区又君,李加儿,等.急性盐度胁迫对卵形鲳鲹幼鱼Na+-K+-ATPase活性和渗透压的影响[J].台湾海峡,2012,31(2):218-224. Bowden T J. Modulation of the immune system of fish by their environment[J]. Fish Shellfish Immunology,2008,25(4): 373-383. Wei L Z,Zhang X M,Huang G Q,et al. Effects of Limited Dissolved Oxygen Supply on the Growth and Energy Allocation of Juvenile Chinese Shrimp,Fenneropenaeus chinensis[J]. World Aquacult Soc,2009,40(4): 483-492. 赵慧慧,吴垠,王炳刚,等.循环养殖系统中溶氧水平对虹鳟消化酶活性及消化吸收率的影响[J].大连水产学院学报,2007,22(3): 198-202. 王春琳,吴丹华,董天野,等.曼氏无针乌贼耗氧率及溶氧胁迫对其体内酶活力的影响[J].应用生态学报,2008,19(11): 2420-2427. 胡国宏,孙广华,朱世成,等.低溶氧量对怀头鲇呼吸代谢耗氧率的影响[J]. 动物学杂志,2002,37(2): 46-48. 尾崎久雄.鱼类血液与循环生理[M]. 许学龙,等译.上海:上海科学技术出版社,1982:6-96. 林浩然.鱼类生理学[M]. 广州:广东高等教育出版社,1999:123. Andenen D E,Reid S D,Moon T W,et al. Metabolic effects associated with chronically elevated cortisol in rainbow trout (Oncorhynchus mykiss)[J]. Can J Fish Aquat Sci,1991,48(9): 1811-1817. Vijayan M M,Moon T W. Acute handling stress alters hepatic glycogen metabolism in food-deprived rainbow trout (Oncorhynchus mykiss)[J].Can J Fish Aquat Sci,1992,49(11): 2260-2266. Burtis C A,Ashwood E R, Tiet Z,et al. Fundamentals of Clinical Chemistry[M]. Philadelphia: W B Saunders Company,1996. 冀德伟,李明云,王天柱,等. 不同低温胁迫时间对大黄鱼血清生化指标的影响[J].水产科学,2009,28(1): 1-4. 崔杰峰,潘柏申.急性心肌梗死血清酶标志物沿革[J].上海医学检验杂志,2000,15(1): 13-14. Berent D,Schmidt H,Wahli T,et al. Effluent from a sewage treatment works causes changes in serum chemistry of brown trout (Salmo truttal.) [J]. Ecotox Environ Safe,2001,48(2):140-147.
点击查看大图
计量
- 文章访问数: 1411
- HTML全文浏览量: 8
- PDF下载量: 1138
- 被引次数: 0