鳗鲡目鱼类线粒体蛋白质编码基因易位及系统演化关系分析

申欣; 田美; 孟学平; 程汉良; 阎斌伦

doi:10.3969/j.issn.0253-4193.2014.04.009

鳗鲡目鱼类线粒体蛋白质编码基因易位及系统演化关系分析

doi: 10.3969/j.issn.0253-4193.2014.04.009

1.
Jiangsu Key Laboratory of Marine Biotechnology, College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China;Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China
2.
Jiangsu Key Laboratory of Marine Biotechnology, College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China

基金项目: 国家自然科学基金（40906067）；香江学者计划（XJ2012056）；中国博士后科学基金（2012M510054，2012T50218）；中央财政支持地方高校发展专项资金（CXTD04）；江苏省“青蓝工程”人才基金资助项目（苏教师[2010]27号）；江苏高校优势学科建设工程资助项目。

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出版历程
- 收稿日期: 2013-05-22
- 修回日期: 2013-10-15

Eels mitochondrial protein-coding genes translocation and phylogenetic relationship analyses

1.
Jiangsu Key Laboratory of Marine Biotechnology, College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China;Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China
2.
Jiangsu Key Laboratory of Marine Biotechnology, College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China

摘要

摘要: 鳗鲡目鱼类线粒体基因组的基因组成较为保守，在58个线粒体基因组中，仅有3个物种存在基因数量的差异。在19个鳗鲡目鱼类线粒体基因组中存在主编码基因的重排。主编码基因的变异位点分析结果支持 nad5、nad4和nad2基因作为cox1和lrRNA基因辅助的分子标记。鳗鲡科Anguillidae的20个种（亚种）聚在一起，强烈支持鳗鲡科为单系群（BPP=100）。鳗鲡科下属的3个类群（大洋洲类群、大西洋类群和印度洋-太平洋类群）也同时得到有力的验证（BPP均为100）。线鳗科Nemichthyidae和锯齿鳗科Serrivomeridae亲缘关系最近，二者聚类后，与鳗鲡科Anguillidae构成姊妹群（BPP=100）。在囊喉鱼亚目Saccopharyngoidei中，宽咽鱼科Eurypharyngidae与囊鳃鳗科Saccopharyngidae聚类（BPP=100），同时，单颌鳗科Monognathidae与月尾鳗科Cyematidae聚类（BPP=100），4个科聚在一支，支持囊喉鱼亚目为单系群（BPP=100）。在囊喉鱼亚目线粒体基因组中，3个物种（吞鳗Eurypharynx pelecanoides、拉文囊鳃鳗Saccopharynx lavenbergi和杰氏单颌鳗Monognathus jesperseni）存在主编码基因的重排。16种鳗鲡（细美体鳗Ariosoma shiroanago、短吻颈鳗Derichthys serpentinus、凯氏短尾康吉鳗Coloconger cadenati、鸭颈鳗Nessorhamphus ingolfianus、龟草鳗Thalassenchelys sp.、粗犁齿海鳗Cynoponticus ferox、百吉海鳗Muraenesox bagio、巨斑花蛇鳗Myrichthys maculosus、大吻沙蛇鳗Ophisurus macrorhynchos、几内亚副康吉鳗Paraconger notialis、哈氏异康吉鳗Heteroconger hassi、小头鸭嘴鳗Nettastoma parviceps、弱头鳗Leptocephalus sp.、斑点长犁齿鳗Hoplunnis punctata、尖吻小鸭嘴鳗Facciolella oxyrhyncha和星康吉鳗Conger myriaster），与鳗鲡目线粒体主编码基因的原始排列相比，共享nad 6 基因的易位。同时，基于线粒体基因组13个蛋白质编码基因构建的系统演化树，强烈支持这16个物种聚为一支（BPP=100）。然而，由此而带来的海鳗科Muraenesocidae、拟鯙科Chlopsidae和糯鳗科Congridae是否为单系群的问题，值得今后深入探究。
- 鳗鲡 /
- 蛋白质编码基因 /
- 基因易位 /
- 系统演化关系
Abstract: The gene composition of eels mitochondrial genomes is conservative. In 58 mitochondrial genomes, only three ones exist differences of gene number. There are major encoding gene rearrangements in 19 eels mitochondrial genomes. The mutation sites analyses of major encoding genes support that nad5, nad4 and nad2 genes are ideal assistant markers to cox1 and lrRNA genes. Twenty species (subspecies) from Anguillidae are clustered together, which strongly support that the family Anguillidae is a monophyletic group (BPP=100). Three groups (Oceanian group, Atlantic group and the Indo-Pacific group) from Anguillidae are also strong verified (BPP of all three clades are 100). There are closest relationship between Nemichthyidae and Serrivomeridae, and they clustering with a sister group Anguillidae (BPP=100). In the suborder Saccopharyngoidei, Eurypharyngidae is clustering with Saccopharyngidae (BPP=100). Meanwhile, Monognathidae is clustering with Cyematidae (BPP=100), four families are clustered together, which strongly support that the suborder Saccopharyngoidei is one monophyletic group (BPP=100). Within the suborder Saccopharyngoidei, there are major encoding gene rearrangements in three species (Eurypharynx pelecanoides, Saccopharynx lavenbergi and Monognathus jesperseni) mitochondrial genomes. Sixteen eels (Ariosoma shiroanago, Derichthys serpentinus, Coloconger cadenati, Nessorhamphus ingolfianus, Thalassenchelys sp., Cynoponticus ferox, Muraenesox bagio, Myrichthys maculosus, Ophisurus macrorhynchos, Paraconger notialis, Heteroconger hassi, Nettastoma parviceps, Leptocephalus sp., Hoplunnis punctata, Facciolella oxyrhyncha and Conger myriaster) share translocation of nad6 gene, which compared with the Anguilliformes mitochondrial original arrangement. Phylogenetic tree based on the 13 mitochondrial protein-coding genes also strongly support the clustering of them (BPP=100). As a result, however, whether Muraenesocidae, Chlopsidae and Congridae are monophyletic groups or not, which worthy of further exploration.
- eel /
- protein-coding gene /
- gene translocation /
- phylogenetic relationship

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参考文献(20)

Inoue J G,Miya M,Miller M J,et al. Deep-ocean origin of the freshwater eels[J]. Biology Letters,2010,6(3): 363—366.

Lee W C,Chen Y H,Lee Y C,et al. The competitiveness of the eel aquaculture in Taiwan,Japan,and China[J]. Aquaculture,2003,221(1-4): 115—124.

Boore J L,Brown W M. Big trees from little genomes: mitochondrial gene order as a phylogenetic tool[J]. Curr Opin Genet Dev,1998,8(6): 668—674.

Curole J P,Kocher T D. Mitogenomics: digging deeper with complete mitochondrial genomes[J]. Trends Ecol Evol,1999,14(10): 394—398.

Boore J L,Macey J R,Medina M. Sequencing and comparing whole mitochondrial genomes of animals[J]. Methods Enzymol,2005,395: 311—348.

Shen X,Ma X,Ren J,et al. A close phylogenetic relationship between Sipuncula and Annelida evidenced from the complete mitochondrial genome sequence of Phascolosoma esculenta[J]. BMC Genomics,2009,10: 136—146.

Minegishi Y,Aoyama J,Inoue J G,et al. Molecular phylogeny and evolution of the freshwater eels genus Anguilla based on the whole mitochondrial genome sequences[J]. Molecular Phylogenetics and Evolution,2005,34(1): 134—146.

Inoue J G,Miya M,Aoyama J,et al. Complete mitochondrial DNA sequence of the Japanese eel Anguilla japonica[J]. Fisheries Science,2001,67(1): 118—125.

Watanabe S,Aoyama J,Tsukamoto K. A new species of freshwater eel Anguilla luzonensis(Teleostei: Anguillidae) from Luzon Island of the Philippines[J]. Fisheries Science,2009,75(2): 387—392.

Teng H Y,Tzeng C S,Lin Y S. A new Anguilla species and a reanalysis of the phylogeny of freshwater eels[J]. Zoological Studies,2009,48(6): 808—822.

Inoue J G,Miya M,Tsukamoto K,et al. Complete mitochondrial DNA sequence of Conger myriaster(Teleostei: Anguilliformes): novel gene order for vertebrate mitochondrial genomes and the phylogenetic implications for anguilliform families[J]. Journal of Molecular Evolution,2001,52(4): 311—320.

Inoue J G,Miya M,Tsukamoto K,et al. Basal actinopterygian relationships: a mitogenomic perspective on the phylogeny of the "ancient fish"[J]. Molecular Phylogenetics and Evolution,2003,26(1): 110—120.

Inoue J G,Miya M,Tsukamoto K,et al. Mitogenomic evidence for the monophyly of elopomorph fishes (Teleostei) and the evolutionary origin of the leptocephalus larva[J]. Molecular Phylogenetics and Evolution,2004,32(1): 274—286.

Inoue J G,Miya M,Tsukamoto K,et al. Evolution of the deep-sea gulper eel mitochondrial genomes: large-scale gene rearrangements originated within the eels[J]. Molecular Biology and Evolution,2003,20(11): 1917—1924.

Larkin M A,Blackshields G,Brown N P,et al. Clustal W and Clustal X version 2.0[J]. Bioinformatics,2007,23 (21): 2947—2948.

Librado P,Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics,2009,25(11): 1451—1452.

Darriba D,Taboada G L,Doallo R,et al. jModelTest 2: more models,new heuristics and parallel computing[J]. Nature Methods,2012,9(8): 772.

Ronquist F,Teslenko M,van der Mark P,et al. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space[J]. Systematic Biology,2012,61(3): 539—542.

San Mauro D,Gower D J,Zardoya R,et al. A hotspot of gene order rearrangement by tandem duplication and random loss in the vertebrate mitochondrial genome[J]. Molecular Biology and Evolution,2006,23(1): 227—234.

Karmovskaya E S,Merrett N R. Taxonomy of the deep-sea eel genus,Histiobranchus(Synaphobranchidae,Anguilliformes),with notes on the ecology of H. bathybius in the eastern North Atlantic[J]. Journal of Fish Biology,1998,53(5): 1015—1037.

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