基于氧化逆境标志物的北部湾潮间带沉积物有机污染程度综合评价

孟范平; 程凤莲; 王建春; 杜秀萍; 李正炎; 李永富; 周游; 王志峰

doi:10.3969/j.issn.0253-4193.2013.03.018

基于氧化逆境标志物的北部湾潮间带沉积物有机污染程度综合评价

doi: 10.3969/j.issn.0253-4193.2013.03.018

中国海洋大学海洋环境与生态教育部重点实验室, 山东青岛 266100

基金项目: 国家海洋公益性行业科研专项 (201005012)。

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出版历程
- 收稿日期: 2012-03-05
- 修回日期: 2013-02-28

Organic contamination assessment of Beibu Gulf intertidal zone with IBR index based on biomarkers of oxidative stress

Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China

摘要

摘要: 利用综合生物标志物响应(IBR)指数对北部湾潮间带沉积环境中多氯联苯(PCBs)、石油烃(TPH)、壬基酚(NP) 和苯并芘(B[a]P)的综合污染水平进行评价。在9个站位采集波纹巴非蛤(Paphia undulata)和沉积物样品,测定沉积物和生物软组织中有机污染物含量,同时对鳃、内脏中与细胞防御系统有关的各7种标志物(包括还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)等)进行分析。利用相关性分析法筛选出对1~2种有机污染物响应敏感的生物标志物共8种,分别为内脏中的GPx、GSH、GSSG、CAT和鳃中的GPx、GSH、GSSG、CAT。以筛选的8种生物标志物为基础, 9个站位IBR指数范围为0.27~1.48,其中站位S2(湛江市乐民镇码头)、站位S9(湛江市江洪码头)的IBR较小,显示污染程度最轻;站位S4(防城港西湾)、站位S3(钦州湾老人沙浅滩) IBR较高,显示生物体因有机污染产生氧化逆境。不同站位的IBR值与污染源分布、化学污染物分析结果基本一致,表明该方法可用于北部湾潮间带沉积物有机污染程度的综合评价。
- 北部湾 /
- 有机污染 /
- 波纹巴非蛤(Paphiaundulata) /
- 生物标志物 /
- 综合标志物响应(IBR)指数
Abstract: Integrated Biomarker Response (IBR) index was used for accessing the integrated quality impacted by four organic contaminants, total petroleum hydrocarbon (TPH), polychlorinated biphenyls (PCBs), Nonylphenol (NP) and Benzo(a)pyrene (B[a]P) in the intertidal sediment of Beibu Bay. Bivalves Paphia undulata and sediments were collected from 9 sites. Contents of the organic contaminants were analyzed in the sediment and soft tissue samples. Seven biomarkers related to cellular defense systems including reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GPx), catalase (CAT), etc. in the hepatic tissues and gills of bivalves were also quantified respectively. According to correlation analysis, eight biomarkers were selected for calculating IBR value because of their significant response to one or two pollutants in the sediment and bivalve tissues. They were GPx, GSH, GSSG, CAT in the hepatic tissues and GPx, GSH, GSSG, CAT in the gills. The IBR index of 9 sampling sites ranged from 0.27 to 1.48. Higher IBR values at site S4 in the western Fangchenggang Bay and S9 in Laorensha tidal-flat of Qinzhou Bay indicated a situation of oxidative stress in bivalves induced by organic pollutants. on the contrary, site S2 in Lemin Port and S3 in Jianghong Port of Zhanjiang City had lower IBR values indicating little pollution situation. The IBR index data agreed with chemical determination profiles and distribution of contamination sources. Therefore, this method will offer a promising potential for the integrated assessment of organic contamination in intertidal sediment of Beibu Gulf.
- Beibu Gulf /
- organic contamination /
- clam Paphia undulata /
- biomarkers /
- Integrated Biomarker Response (IBR) Index

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参考文献(28)

周驰, 李纯厚. 生物大分子标记物检测在环境监测中的应用[J]. 中国水产科学, 2007, 14(5): 864-871.

Livingstone D R. Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment[J]. Journal of Chemical Technology and Biotechnology, 1993, 57(3): 195-211.

Binelli A, Cogni D, Parolini M, et al. Multi-biomarker approach to investigate the state of contamination of the R. Lambro/R. Po confluence (Italy) by zebra mussel (Dreissena polymorpha)[J]. Chemosphere, 2010, 79(5): 518-528.

Broeg K, Lehtonen K K. Indices for the assessment of environmental pollution of the Baltic Sea coasts: Integrated assessment of a multi-biomarker approach[J]. Marine Pollution Bulletin, 2006, 53(8/9): 508-522.

Pytharopoulou S, Sazakli E, Grintzalis K, et al. Translational responses of Mytilus galloprovincialis to environmental pollution: Integrating the responses to oxidative stress and other biomarker responses into a general stress index[J]. Aquatic Toxicology, 2008, 89(1): 18-27.

Oliveira M, Maria V L, Ahmad I, et al. Contamination assessment of a coastal lagoon (Ria de Aveiro, Portugal) using defence and damage biochemical indicators in gill of Liza aurata-An integrated biomarker approach[J]. Environmental Pollution, 2009, 157(3): 959-967.

Damiens G, Gnassia-Barelli M, Loque's F, et al. Integrated biomarker response index as a useful tool for environmental assessment evaluated using transplanted mussels[J]. Chemosphere, 2007, 66(3): 574-583.

Cheung C H Y, Chaillé P M, Randall D J, et al. The use of scale increment as a means of indicating fish growth and growth impairment[J]. Aquaculture, 2007, 266(1/4): 102-111.

中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 海洋监测规范(GB 17378-2007)[S]. 北京: 中国标准出版社, 2008.

周传光, 徐恒振, 马永安, 等. 毛细管GC-ECD测定环境样品中的PCBs[J]. 海洋环境科学, 2000, 19(4): 57-61.

汪正范. 色谱定性与定量[M]. 北京: 化学工业出版社, 2000: 170-172.

Li D H, Oh J R, Park J. Direct extraction of alkylphenols, chlorophenols and bisphenol A from acid-digested sediment suspension for simultaneous gas chromatographic-mass spectrometric analysis[J]. Journal of Chromatography A, 2003, 1012(2): 207-214.

Wang J, Dong M H, Shim W J, et al. Improved cleanup technique for gas chromatographic-mass spectrometric determination of alkylphenols from biota extract[J]. Journal of Chromatography A, 2007, 1171(1/2): 15-21.

US Environmental Protection Agency. Method 8100, Polynuclear Aromatic Hydrocarbons[S]. Washington, D C, 1986.

高鹰. 海水铜铅及环境因子对菲律宾蛤仔生物标志物的影响研究[D]. 青岛:中国海洋大学, 2011.

Habig W H, Jakoby W B. Assays for differentiation of glutathione S-transferase[J]. Methods in Enzymology, 1981, 77: 398-405.

汤建林, 周世文, 徐传福. 分光光度法测定小鼠组织中谷胱甘肽过氧化物酶活力[J]. 第三军医大学学报, 1996, 6: 551-552.

张迺哲, 宋玉忱. 组织中氧化型和还原型谷胱甘肽荧光测定法[J]. 生物化学与生物物理进展, 1993, 2: 136-138.

Beliaeff B, Burgeot T. Integrated biomarker response: a useful tool for ecological risk assessment[J]. Environmental Toxicology and Chemistry, 2002, 21(6): 1316-1322.

Donkin P, Widdows J, Sheila V, et al. Quantitative structure-activity relationships for the effect of hydrophobic organic chemicals on rate of feeding by mussels (Mytilus edulis)[J]. Aquatic Toxicology, 1989, 14(3): 277-293.

Livingstone D R. Oxidative stress in aquatic organisms in relation to pollution and aquaculture[J]. Revue de Médecine Vétérinaire, 2003, 154 (6): 427-430.

Vander Oost R, Beyer J, Vermeulen N P E. Fish bioaccumulation and biomarkers in environmental risk assessment: a review[J]. Environmental Toxicology and Pharmacology, 2003, 13(2): 57-149.

Pan L Q, Ren J Y, Liu J. Effects of benzo(k)fluoranthene exposure on the biomarkers of scallop Chlamys farreri[J]. Comparative Biochemistry and Physiology, Part C, 2005, 141(3): 248-256.

Soldatov A A, Gostyukhina O L, Golovina I V. Antioxidant enzyme complex of tissues of the bivalve Mytilus galloprovincialis Lam. under normal and oxidative-stress conditions: a review[J]. Applied Biochemistry and Microbiology, 2007, 43(5): 556-562.

Vieira L R, Sousa A, Frasco M F, et al. Acute effects of benzo[a]pyrene, anthracene and a fuel oil on biomarkers of the common goby Pomatoschistus microps (Teleostei, Gobiidae)[J]. Science of the total Environment. 2008, 295(2/3): 87-100.

Pichaud N, Pellerin J, Fournier M, et al. Oxidative stress and immu???????????扰牯?孳??崠??桬敬畯湷杩????????却楡畲?圠??????割楥挠桴慯爠摐獁潈湳??????敭琾?慹污???湥瑮楡潲硩楡搼愯湥瑭￣牛敊獝瀮漠湃獨敥獭?瑳潴?批攠湃穥潮孴慲嵡灬礠牊敯湵敲?慡湬搬??爰漰挸氬漠爲?ㄠ有???敢硲瀾潛猲甸牝攠?楥湮?瑤桥整?杩爠敍攬渠?汩楡灰灲敩摮?洠畆猬猠敐汩???敆洬?健整爠湡慬?瘠楁爠業摵楬獴??敩浳?孩?嵬???湲癹椠牷潥湩浧敨湴琠慯汦?健潶汩汤略瑮楣潥渠???ひは???ㄠ????????????ど???扰牯?孬??嵥??敳牥牤敩業牥慮?????潮牴慥摧慲獡??敮牧爠敳楥牤慩?健??删散楨獥??敳湴牲楹焬甠敢獩??????佡硢楩摬慩瑴楹瘬攠?獩瑯牭敡獲獫?扲楳漠浲慥牳歰敯牮獳?楳渠?瑮睤漠?物敯獡楳摳敡湹瑳?獊灝攮挠楅敮獶??浯畮汭汥敮瑴???整浥??畡杴楩汯?捡敬瀬栠愲氰由猲??攳洸???愺渠搱?昭氲漸甮渼摢敲爾???敝洠?偯汷慬瑡楮捤栠瑂栠祔猠?昬氠敍獣畉獮??敳浨????昬爠潄浡?慩?灳漠汉氠畍琬攠摥?猠楡瑬攮?楉湭?剬楩癣敡牴??潮畳爠潦??獭琠畡愠牦祩??偤漠牳瑴畵杤慹氠孲?嵧???煩畮慧琠楴捨?吠潲硥楬捡潴汩潯杮祳????ぢ??????ㄠ??????????戠牡?孯??嵴??甠捨慹??扯扣潡瑲瑢?卮????敤??剬極捴桡慴牨摩獯潮湥???????捳?汥敲污汳慥渠??????整瑹?慩汮???楳敳汥摬?癛慊汝椮搠慍瑡楲潩湮?漠晅?慶湩瑲楯潮硭楥摮慴湡瑬?敒湥穳祥浡敲?扨椠漲洰愰爲欬攠爵猴?椳港‵洩町猠猲攳氱猭′??攮洼?偲放牛渳愰?瘠楒物楣摨楡獲??敯浮???慊測搠?捡汫愠浅猬???散浡?剁畢摢楯瑴慴瀠敓獨?灲桯楮氠楂瀠灄楥測愠牥畴洠??攮洠???瑩牯慸湩獤灡汮慴渠瑲敥摳?楯湮??潳渠杴??潰湯杬?捣潹慣獬瑩慣氠?睲慯瑭敡牴獩季?嵨???慯牣楡湲敢?偮潳氠污畮瑤椠潯湲??畮汯汣敨瑬楯湲????ば?????????㈠????????????扰牥?嬠??嵳??慬瑳漠稨稼潥?嘾???慮污氠慶物楲湩?????慥牭椾温???????硳灳潥獬畳爠攢?潮晴?瑧桲敡?捥氢愠浢??敭浡?呫慥灲攠獲?灳桰楯汮楳灥灳椿湛慊牝甮浍??敩浮??瑐潯???湴潩湯祮氠灂桵敬湬潥汴??挬栠愲渰朰攸猬?椵渷?愶港琱椲?漺砠椵搰愳渭琵?攴渮稼祢浲放?愳挱瑝椠癒楡瑭楯敳猭?愦渣搲?爳攻?扥畺爠牊漬眠楃湯杺?捁愬瀠慖扩楧汵楲瑩礠孊?嵒???慴爠楡湬攮?偂潩汯汭畡瑲楫潥湲??略汳汰敯瑮楳湩???づび?????????????????????扳爠?学??嵡?剥楤瘠愼????偵潤物瑴敡?????楨湩敬汩汰楰????敵瑭?愯汥?￣?癡慮汤甠慩瑴楳漠湵?潥映???湨潩湮礠污灮栠敩湮潴汥?楲湡?癥楤瘠潳?敤硩灭潥獮畴爠敱?楡湬??敹洠??牳敥楳獳獭敥湮慴?灊潝氮礠浅潮牶灩桲慯??敥浮????楐潯慬捬捵畴浩畯汮愬琠椲漰渱??猠琱攵爹漨椷搩?氠攱瘹攱水猭?愹渲搲?漼硢楲搾慛琳椲癝攠?獥瑧牯敬獩猠孆?崠???潮浣灩慰牡慴瑯楢瘠敇??楇潬捵桴敡浴楨獩瑯牮祥?愠湧摬?側桡祴獨楩潯汮潥札祤??健慮牤瑥?????つㄠち??????????ㄠ????????扩牮?孭?び嵳??愬椠???堾??兴楩?卵????婬敬湯杰???奩??敩瑡?慩汳???業猾琬爠楥扸異瑯楳潥湤?潴景?灭潥汴祡捬祳挠汵楮捤?慲爠潦浩慥瑬楤挠?桮祤搠牬潡换慯牲扡潴湯獲?椠湣?瑮桤敩?捩潯慮獳琺愠汩?牰敬杩楣潡湴?潯普晳??慯捲愠潴???桵楳湥愠??愠獢獩敯獣獨浥敭湩瑣?潬映?楩湯灭畡瑲?獥潲畳牛捊敝献?慁湱摵?瑴物慣渠獔灯潸物瑣?灬慯瑧桹眬愠礱猹?电猬椠渳朱?挲漩洺瀠漱猴椳琭椱漶渴愮氼?慲渾慛氳礳獝椠獃孨?嵵???湃瘠楃爠潃測洠敚湨瑥慮汧?升挠楊攬渠捌敩?呁攠捍栠湙漬氠潥杴礠??金?げ?????????????????????tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis[J]. Aquatic Toxicology, 2001, 52(1/12): 189-203.

Niyogia S, Biswasa S, Sarkerb S, et al. Antioxidant enzymes in brackishwater oyster, Saccostrea cucullata as potential biomarkers of polyaromatic hydrocarbon pollution in Hooghly Estuary (India): seasonality and its consequences[J]. The Science of the Total Environment, 2001, 281(1/3)

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