海水中丙烯酸的高效液相色谱法建立及应用

刘春颖; 王莉莉; 杨桂朋; 陈岩; 李培峰

doi:10.3969/j.issn.0253-4193.2013.01.020

海水中丙烯酸的高效液相色谱法建立及应用

doi: 10.3969/j.issn.0253-4193.2013.01.020

中国海洋大学化学化工学院海洋化学理论与工程技术教育部重点实验室, 山东青岛266100

基金项目: 国家自然科学基金(41176062;40706040;41030858);山东省自然科学基金(Y2008E04);教育部"长江学者"奖励计划和山东省"泰山学者"建设工程资助项目。

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出版历程
- 收稿日期: 2012-03-02
- 修回日期: 2012-07-23

Determination of acrylic acid in seawater by high performance liquid chromatography and its application

Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China

摘要

摘要: 建立了一种测定海水介质中丙烯酸的高效液相色谱法。采用耐纯水相和较低pH的Agilent SB-Aq-C18柱(100 mm×4.6 mm i.d.,5 μm), 0.35%磷酸溶液(pH=2)为流动相,使用紫外检测器,检测波长为210 nm,外标法定量测定。丙烯酸的保留时间在14.2~ 14.9 min,方法的检出限为4 nmol/dm³(S/N=3),在0.01~10 μmol/dm³的范围内均有良好的线性关系,相关系数可达0.999 6,加标回收率为95.4%~98.1%,相对标准偏差为1.3%~1.6%(1.04~2.32 μmol/dm³)。采用0.2 μm滤膜重力过滤冷藏的办法来保存海水丙烯酸样品。检测出2011年5月份青岛近海海水中丙烯酸的平均浓度为(0.101±0.069) μmol/dm³;海洋微藻球形棕囊藻在整个生长周期内培养体系中丙烯酸的浓度为0.339~2.219 μmol/dm³。
- 高效液相色谱法 /
- 丙烯酸 /
- 海水
Abstract: A reversed phase high performance liquid chromatographic method was developed to determine dissolved acrylic acid in seawater. Using the Agilent SB-Aq-C18 column (100 mm×4.6 mm i.d., 5 μm) and 0.35% H₃PO₄-water (pH=2) as the mobile phase, the column eluate was monitored by ultraviolet detector. The wavelength of UV detector was 210 nm, and an external standard method was adopted. The detection limit of the method was 4 nmol/dm³(S/N=3). The peak area is linear with acrylic acid concentration in the range of 0.01~10 μmol/dm³, with a correlation coefficient of 0.999 6. The average recovery was 95.4%~98.1% and RSD was 1.3%~1.6%. Samples for acrylic acid could be stored in the dark at 4℃ after gravity filter through 0.2 μmWhatman membrane. The highly sensitive and accurate analytical method has been successfully applied to the determination of acrylic acid in natural seawater and algal culture.
- high performance liquid chromatography /
- acrylic acid /
- seawater 

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