海洋酸化与磷限制对颗石藻（<i>Emiliania huxleyi</i>）生理特征的耦合效应

马帅; 张泳; 杨祥; 王茵蕊; 谢蓉蓉; 李家兵; 张虹; 韩永和; 张勇

doi:10.12284/hyxb2023108

海洋酸化与磷限制对颗石藻（Emiliania huxleyi）生理特征的耦合效应

doi: 10.12284/hyxb2023108

马帅^1,,
张泳¹,
杨祥¹,
王茵蕊¹,
谢蓉蓉^{1, 2},
李家兵^{1, 2},
张虹^{1, 2},
韩永和^{1, 2},
张勇^{1, 2, ,}

1.
福建师范大学环境与资源学院、碳中和现代产业学院，福建福州 350117
2.
福建师范大学福建省污染控制与资源循环利用重点实验室，福建福州 350117

基金项目: 福建省自然科学基金面上项目（2023J01290）；国家自然科学基金青年项目（41806129）。

详细信息

作者简介:
马帅（1997-），男，河南省长葛市人，主要从事海洋藻类生理学研究。E-mail: ms1471692021@163.com

通讯作者:
张勇，男，副研究员，主要研究海洋微藻响应气候变化的生理生态特征及其机制。E-mail: yongzhang@fjnu.edu.cn

中图分类号: P714⁺.5；Q949.2
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- 被引次数: 0
出版历程
- 收稿日期: 2023-01-10
- 修回日期: 2023-04-25
- 网络出版日期: 2023-08-31
- 刊出日期: 2023-08-31

Combined effects of ocean acidification and phosphorus limitation on physiological characteristics of the coccolithophores Emiliania huxleyi

Ma Shuai^1
,,
Zhang Yong¹,
Yang Xiang¹,
Wang Yinrui¹,
Xie Rongrong^{1, 2},
Li Jiabing^{1, 2},
Zhang Hong^{1, 2},
Han Yonghe^{1, 2},
Zhang Yong^{1, 2
, ,}

1.
College of Environmental and Resource Sciences · College of Carbon Neutral Modern Industry, Fujian Normal University, Fuzhou 350117, China
2.
Fujian Key Laboratory of Pollution Control and Resource Recycling, Fujian Normal University, Fuzhou 350117, China

摘要

摘要: 颗石藻既通过光合作用合成颗粒有机碳（POC），又通过钙化作用产生颗粒无机碳（PIC），对海洋碳循环有重要贡献。虽然已有一些研究报道海洋酸化通常可增加颗石藻细胞POC含量，减少细胞PIC含量，但是这些实验结果大部分是在营养盐浓度充足条件下获得，且较少研究关注营养盐浓度限制和海洋酸化对颗石藻的耦合作用。本文研究了在无机磷浓度限制条件下，赫氏颗石藻（Emiliania huxleyi）的主要生理指标对海洋酸化的响应特征。结果显示，海洋酸化和无机磷浓度限制协同降低了颗石藻的生长速率、相对电子传递速率和光能利用效率。无机磷浓度限制主导增加颗石藻的光合固碳量，并抵消了海洋酸化对细胞PIC含量及PIC/POC比率的负面效应。研究结果表明，无机磷浓度限制改变了颗石藻的两种固碳作用对海洋酸化的响应趋势，暗示在无机磷浓度不同的海域，颗石藻对海洋碳循环的贡献不同。
- 颗石藻 /
- 赫氏颗石藻 /
- 海洋酸化 /
- 磷限制 /
- 生长速率 /
- 钙化作用 /
- 光合作用
Abstract: Coccolithophores can produce particulate organic carbon (POC) via photosynthesis and synthesize particulate inorganic carbon (PIC) via calcification; they also make an important contribution to marine carbon cycle. Some studies have reported that ocean acidification generally increases POC content and decreases PIC content of coccolithophores, however, most of the studies show these results under nutrient replete conditions, and a few studies focus on the combined effects of nutrient limitation and ocean acidification on coccolithophores. In this study, we investigated the physiological responses of the coccolithophore Emiliania huxleyi to ocean acidification under dissolved inorganic phosphorus (DIP) limitation. Our data show that ocean acidification and DIP limitation act synergistically to reduce growth rate, relative photosynthetic electron transport rate and light use efficiency of photosynthetic system II of E. huxleyi. Dissolved inorganic phosphorus limitation dominantly increases cellular POC content and offsets the negative effect of ocean acidification on cellular PIC content and the ratio of PIC : POC. Our results suggest that DIP limitation changed the response trends of POC and PIC contents to ocean acidification, which indicates that the contributions of coccolithophores to marine carbon cycle are different in these ocean areas with different DIP concentrations.
- coccolithophore /
- Emiliania huxleyi /
- ocean acidification /
- phosphorus limitation /
- growth rate /
- calcification /
- photosynthesis

HTML全文

图 1 不同处理下，在培养开始和结束时，培养基中溶解无机磷（DIP）浓度（A−D）、CO₂分压（E−H）、pH（I−L）和细胞浓度（单位：cell/mL）的自然对数（M−P）

Fig. 1 Dissolved inorganic phosphorus (DIP) concentration (A−D), pCO₂ level (E−H), pH (I−L) and logarithm of cell concentration (unit: cell/mL) (M−P) at the beginning and end of the cultures under different treatments

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图 2 不同处理下赫氏颗石藻（Emiliania huxleyi）株系RCC1266的生长速率（A）、叶绿素a含量（B）、类胡萝卜素含量（C）及类胡萝卜素与叶绿素a含量的比率（D）

不同字母代表显著性差异

Fig. 2 Growth rate (A), chlorophyll a content (B), carotenoid content (C) and the ratio of carotenoid content to chlorophyll a content (D) of the coccolithophore Emiliania huxleyi RCC1266 under different treatments

Different letters represent significant difference

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图 3 不同处理下赫氏颗石藻（Emiliania huxleyi）株系RCC1266相对电子传递速率（rETR）对检测光强的响应曲线（A）、最大相对电子传递速率（rETR_max）（B）、光能利用效率（α）（C）和半饱和光强（I_k）（D）

不同字母表示显著性差异

Fig. 3 Relative electron transport rate (rETR) as a function of the assay light intensity (A), maximal relative electron transport rate (rETR_max) (B), light use efficiency (α) (C) and half-saturating light intensity (I_k) (D) of the coccolithophore Emiliania huxleyi RCC1266 under different treatments

Different letters represent significant difference

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图 4 不同处理下赫氏颗石藻（Emiliania huxleyi）株系RCC1266细胞颗粒无机碳（PIC）含量（A）、颗粒有机碳（POC）含量（B）和颗粒无机碳与颗粒有机碳（PIC/POC）的比率（C）

不同字母代表显著性差异

Fig. 4 The cellular particulate inorganic carbon (PIC) content (A), particulate organic carbon (POC) content (B) and the ratio of PIC to POC (PIC/POC) (C) of the coccolithophore Emiliania huxleyi RCC1266 under different treatments

Different letters represent significant difference

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表 1 二元方差分析获得无机磷浓度和CO₂浓度及其相互作用对赫氏颗石藻（Emiliania huxleyi）株系RCC1266的生长速率、色素含量、荧光参数、细胞元素含量及其比率影响的统计结果

Tab. 1 Results of two-way ANOVA of the effects of inorganic phosphorus concentration and CO₂ concentration and their interactions on growth rate, pigment content, fluorescence parameters, cellular elements content and their ratios of the coccolithophore Emiliania huxleyi RCC1266

	无机磷浓度		CO₂浓度		无机磷浓度和CO₂浓度
	F	p	F	p	F	p
生长速率/d⁻¹	281.76	< 0.001	218.75	< 0.001	196.08	< 0.001
叶绿素a含量/( pg·cell⁻¹)	0.14	0.720	9.60	0.015	12.51	0.008
类胡萝卜素含量/( pg·cell⁻¹)	12.70	0.007	6.48	0.034	17.94	0.003
类胡萝卜素与叶绿素a含量的比率	8.44	0.020	2.70	0.139	1.04	0.338
最大相对电子传递速率	29.99	< 0.001	239.26	< 0.001	157.10	< 0.001
光能利用效率	136.70	< 0.001	229.46	< 0.001	343.01	< 0.001
半饱和光强/(μmol ·m⁻² ·s⁻¹)	8.53	0.019	0.01	0.949	7.61	0.025
颗粒无机碳含量/( pg·cell⁻¹)	42.52	< 0.001	18.26	0.003	1.53	0.251
颗粒有机碳含量/( pg·cell⁻¹)	68.46	< 0.001	2.75	0.136	0.10	0.759
颗粒无机碳与颗粒有机碳含量的比率	12.11	0.008	16.94	0.003	1.68	0.231
注：统计学的显著性差异通过p < 0.05来表达，此处的p值是数据整体分析的结果；颗粒无机碳含量和颗粒有机碳含量以碳计。

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