平潭海域夜光藻对几种饵料藻的利用与生长

张海艳; 李晓东; 杨桂琴; 路玮静; 常阳; 王紫阳

doi:10.12284/hyxb2022156

平潭海域夜光藻对几种饵料藻的利用与生长

doi: 10.12284/hyxb2022156

福建农林大学海洋研究院福建省海洋生物技术重点实验室，福建福州 350002

基金项目: 国家自然科学基金（42106203）；平潭科技研究院科技创新项目（PT2021002）。

详细信息

作者简介:
张海艳（1998-），女，云南省弥勒市人，从事平潭夜光藻藻华形成机制相关的研究。E-mail：2484313403@qq.com

通讯作者:
李晓东，男，副教授，主要研究有害藻华形成机制及对海洋生态环境及水产养殖业的毒性效应与机制。E-mail：lixiaodong11@163.com

中图分类号: X55；Q949.2
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出版历程
- 收稿日期: 2021-11-19
- 修回日期: 2022-04-13
- 网络出版日期: 2022-04-28
- 刊出日期: 2022-08-15

Different growth of red Noctiluca scintillans from Pingtan coast waters feeding on several diet algae

Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China

摘要

摘要: 夜光藻（Noctiluca scintillans）藻华是我国近海常见的生态灾害，其暴发机制尚不明确。为探究营养条件在其形成机制中的作用，本文分析了几种常见饵料藻与同海域硅藻培养下夜光藻的种群增长，同时探究模拟海水混合与营养盐水平对其种群增长的影响。结果发现，不同饵料藻培养下，夜光藻种群增长有着显著差异，其中亚心形扁藻（Platymonas subcordiformis）组中夜光藻的平均增长率为（0.151±0.001）d⁻¹，远高于其他饵料藻。海水混合对夜光藻种群增长有一定的促进作用，模拟海水混合时，部分实验组中夜光藻增长率显著高于静置实验组，其中微小海链藻（Thalassiosira minima）培养下的夜光藻种群增长率为（0.136±0.001）d⁻¹，远高于除亚心形扁藻以外的其他实验组。随着营养盐水平的增加，与亚心形扁藻共培养的夜光藻种群增长出现明显的先增强后减弱的趋势，拟合结果表明，其种群增长符合Boltzmann模型，并与营养盐水平相关。结果表明，海水混合与营养盐输入引起的平潭海域微藻的快速繁殖在当地夜光藻藻华的形成机制中可能起着重要的作用。
- 夜光藻 /
- 种群增长 /
- 藻华机制 /
- 营养盐水平
Abstract: Blooms of red Noctiluca scintillans is an annual eco-disaster in coastal waters of China, but the mechanism of outbreak of N. scintillans is still unclear. To explore the role of nutrient conditions in the formation of N. scintillans blooms, the population growth of N. scintillans feeding several diet algae, including common diet algae and local diatoms, were investigated under standing water, mixing water and different inorganic nutrient levels. Results showed that there were significant differences in population growth of N. scintillans under different diets. Population growth of N. scintillans was fastest in Platymonas subcordiformis treatment, with growth rate of (0.151±0.001) d⁻¹. N. scintillans growth significantly increased in mixing water when feeding on some diet algae. The population growth rate of N. scintillans in Thalassiosira minima treatment was (0.136±0.001) d⁻¹, which was much higher than growth rate of N. scintillans in other algae treatment except in P. subcordiformis treatment. Growth rate and maximum population of N. scintillans cultured with P. subcordiformis firstly increased and then decreased with increasing inorganic nutrient levels, and results suggested that its growth fitted the Boltzmann model, with parameters were highly related to inorganic nutrients. Results indicated that proliferation of local diet algae, such as Thalassiosira species, leading by nutrients input in spring, combined with mixing of seawater, might play an important role in the formation of massive N. scintillans bloom in Pingtan coastal waters.
- Noctiluca scintillans /
- population growth /
- bloom mechanism /
- inorganic nutrient levels

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图 1 夜光藻采样站位

Fig. 1 The sampling position of Noctiluca scintillans

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图 2 静置条件下不同藻类培养的夜光藻种群增长

Fig. 2 Population growth of Noctiluca scintillans feeding by different algae in a standing situation

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图 3 海水混合条件下不同藻类培养的夜光藻种群增长

Fig. 3 Population growth of Noctiluca scintillans feeding by different algal in a seawater mixing simulated situation

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图 4 夜光藻和亚心形扁藻在不同营养条件下的种群增长曲线

Fig. 4 Population growth curves of Noctiluca scintillans and Platymonas subcordiformis under different nutritional conditions

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图 5 夜光藻种群动态拟合

Fig. 5 Fitting of population dynamics of Noctiluca scintillans

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表 1 不同培养条件下夜光藻种群平均增长率

Tab. 1 Mean growth rate of Noctiluca scintillans feeding by different algal in standing and mixing simulated situation

饵料藻类	静置条件下增长率/d⁻¹	海水混合条件下增长率/d⁻¹
亚心形扁藻	0.151±0.001^d	0. 152±0^d
冷冻亚心形扁藻	0.106±0.005^b	0.105±0.004^b
球等鞭金藻	0.003±0.003^a	0.101±0.002^b
三角褐指藻	0.013±0.006^a	0.101±0.004^b
微小海链藻	0.005±0.005^a	0.137±0.001^c
小球藻	−0.002±0.003^a	−0.002±0.003^a
骨条藻	0.108±0.003^b	0.105±0.002^b
空白对照（灭菌海水）	0.003±0.003^a	0.000±0.005^a
注：上标字母不同表示差异显著，p<0.05为显著。

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表 2 夜光藻种群动态Boltzmann拟合参数

Tab. 2 Parameters of Boltzmann fitting on population dynamics of Noctiluca scintillans

	f/2组	f/4组	f/8组	f/16组	f/32组	对照组
K/(cells·mL⁻¹)	44.61±4.11	164.56±5.43	184.65±3.19	103.74±2.38	77.22±2.29	46.53±1.61
t₀/d	12.67±0.88	11.05±0.31	7.79±0.17	7.19±0.20	6.96±0.31	6.48±0.36
dt	3.23±0.53	2.56±0.23	1.73±0.15	1.12±0.17	1.71±0.27	1.57±0.31
R²	0.929 3	0.979 1	0.983 3	0.961 9	0.942 9	0.912 5

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表 3 夜光藻种群动态Boltzmann拟合参数与营养盐稀释倍数的拟合

Tab. 3 Fitting of dilution ratio with parameters of Boltzmann fitting on population dynamics of Noctiluca scintillans

	拟合方式	拟合参数				R²
K	高斯	B=41.00±6.69	x_c=0.36±0.01	w=0.32±0.02	A=72.76±5.76	0.994 0
t₀	线性	a=6.57±0.39	b=6.57±0.82			0.941 7
dt	线性	c=1.38±0.18	d=1.90±0.38			0.863 0

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